Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production
Background: Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are mo...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345820300403 |
| _version_ | 1818870850176155648 |
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| author | Feng Li Yingjie Xie Xiang Gao Mingxu Shan Changchao Sun Yan Dong Niu Anshan Shan |
| author_facet | Feng Li Yingjie Xie Xiang Gao Mingxu Shan Changchao Sun Yan Dong Niu Anshan Shan |
| author_sort | Feng Li |
| collection | DOAJ |
| description | Background: Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production. Results: In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively. Conclusions: Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.How to cite: Li F, Xie Y, Gao X, et al. Screening of cellulose degradation bacteria from Min Pigs and optimization of its cellulase production. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.001 |
| first_indexed | 2024-12-19T12:13:34Z |
| format | Article |
| id | doaj.art-d4d8be6216734d73a64d98852c864444 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-19T12:13:34Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-d4d8be6216734d73a64d98852c8644442022-12-21T20:22:05ZengElsevierElectronic Journal of Biotechnology0717-34582020-11-01482935Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase productionFeng Li0Yingjie Xie1Xiang Gao2Mingxu Shan3Changchao Sun4Yan Dong Niu5Anshan Shan6Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR China; Corresponding authors.Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR ChinaInstitute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR ChinaInstitute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR ChinaInstitute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR ChinaDepartment of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 1N4, CanadaInstitute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, PR China; Corresponding authors.Background: Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production. Results: In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively. Conclusions: Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.How to cite: Li F, Xie Y, Gao X, et al. Screening of cellulose degradation bacteria from Min Pigs and optimization of its cellulase production. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.001http://www.sciencedirect.com/science/article/pii/S0717345820300403Animal productionCellulase probioticsCellulase producing bacteriaCellulolytic bacteriaCelluloseDietary fiber |
| spellingShingle | Feng Li Yingjie Xie Xiang Gao Mingxu Shan Changchao Sun Yan Dong Niu Anshan Shan Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production Electronic Journal of Biotechnology Animal production Cellulase probiotics Cellulase producing bacteria Cellulolytic bacteria Cellulose Dietary fiber |
| title | Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production |
| title_full | Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production |
| title_fullStr | Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production |
| title_full_unstemmed | Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production |
| title_short | Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production |
| title_sort | screening of cellulose degradation bacteria from min pigs and optimization of its cellulase production |
| topic | Animal production Cellulase probiotics Cellulase producing bacteria Cellulolytic bacteria Cellulose Dietary fiber |
| url | http://www.sciencedirect.com/science/article/pii/S0717345820300403 |
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