Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae)
Pseudobagrus vachellii (Richardson, 1846) is a commercially important freshwater fish species in China. To understand the effects of artificial breeding on the genetic diversity of three consecutive P. vachellii breeding populations (F1, F2, and F3) since 2012, a genetic analysis was conducted using...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2024-01-01
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| Series: | Acta Ichthyologica et Piscatoria |
| Online Access: | https://aiep.pensoft.net/article/105627/download/pdf/ |
| _version_ | 1797337910121332736 |
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| author | Huan Wang Guoqing Duan Huaxing Zhou |
| author_facet | Huan Wang Guoqing Duan Huaxing Zhou |
| author_sort | Huan Wang |
| collection | DOAJ |
| description | Pseudobagrus vachellii (Richardson, 1846) is a commercially important freshwater fish species in China. To understand the effects of artificial breeding on the genetic diversity of three consecutive P. vachellii breeding populations (F1, F2, and F3) since 2012, a genetic analysis was conducted using polymorphic microsatellite markers. The mean allele number, expected heterozygosity, observed heterozygosity, and the polymorphic information content from generation F1 to F3 decreased from 7.75 to 5.63, from 0.77 to 0.63, from 0.83 to 0.77, and from 0.72 to 0.58, respectively. Analysis of molecular variance showed greater genetic divergence within the three generations (93.67%) than that among the generations (6.33%), and the overall differentiation level was moderate. Additionally, the lowest genetic differentiation was between F2 and F3 (Fst = 0.0484), and the highest was between F1 and F3 (Fst = 0.12864). Inbreeding occurred in each generation and was the highest in generation F3. Structural analysis showed that the three P. vachellii generations were most likely divided into two different genetic clusters. Although genetic diversity declined slightly in the mass selection lines after three breeding generations, overall genetic diversity was maintained at a relatively high level. To minimize the loss of genetic diversity and inbreeding in the subsequent breeding process, a moderate number of parents can be used for each generation. Information regarding the genetic diversity and structure of the selective P. vachellii breeding generations obtained in this study will be useful for future broodstock management and selective breeding programs. |
| first_indexed | 2024-03-08T09:23:24Z |
| format | Article |
| id | doaj.art-d4e2adf087114e918051126eb940b142 |
| institution | Directory Open Access Journal |
| issn | 1734-1515 |
| language | English |
| last_indexed | 2024-03-08T09:23:24Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Acta Ichthyologica et Piscatoria |
| spelling | doaj.art-d4e2adf087114e918051126eb940b1422024-01-31T10:47:09ZengPensoft PublishersActa Ichthyologica et Piscatoria1734-15152024-01-0154132010.3897/aiep.54.105627105627Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae)Huan Wang0Guoqing Duan1Huaxing Zhou2Anhui Province Key Laboratory of Aquaculture and Stock EnhancementAnhui Province Key Laboratory of Aquaculture and Stock EnhancementAnhui Province Key Laboratory of Aquaculture and Stock EnhancementPseudobagrus vachellii (Richardson, 1846) is a commercially important freshwater fish species in China. To understand the effects of artificial breeding on the genetic diversity of three consecutive P. vachellii breeding populations (F1, F2, and F3) since 2012, a genetic analysis was conducted using polymorphic microsatellite markers. The mean allele number, expected heterozygosity, observed heterozygosity, and the polymorphic information content from generation F1 to F3 decreased from 7.75 to 5.63, from 0.77 to 0.63, from 0.83 to 0.77, and from 0.72 to 0.58, respectively. Analysis of molecular variance showed greater genetic divergence within the three generations (93.67%) than that among the generations (6.33%), and the overall differentiation level was moderate. Additionally, the lowest genetic differentiation was between F2 and F3 (Fst = 0.0484), and the highest was between F1 and F3 (Fst = 0.12864). Inbreeding occurred in each generation and was the highest in generation F3. Structural analysis showed that the three P. vachellii generations were most likely divided into two different genetic clusters. Although genetic diversity declined slightly in the mass selection lines after three breeding generations, overall genetic diversity was maintained at a relatively high level. To minimize the loss of genetic diversity and inbreeding in the subsequent breeding process, a moderate number of parents can be used for each generation. Information regarding the genetic diversity and structure of the selective P. vachellii breeding generations obtained in this study will be useful for future broodstock management and selective breeding programs.https://aiep.pensoft.net/article/105627/download/pdf/ |
| spellingShingle | Huan Wang Guoqing Duan Huaxing Zhou Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) Acta Ichthyologica et Piscatoria |
| title | Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) |
| title_full | Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) |
| title_fullStr | Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) |
| title_full_unstemmed | Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) |
| title_short | Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae) |
| title_sort | genetic diversity of three consecutive selective breeding generations in pseudobagrus vachellii actinopterygii siluriformes bagridae |
| url | https://aiep.pensoft.net/article/105627/download/pdf/ |
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