Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens
Foodborne pathogen is an important factor affecting food safety. With the biotechnology development and the improved requirements for food safety, the detection methods of foodborne pathogens are constantly updated and improved. By microfluidic technology, the processes of sample pretreatment, separ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-09-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110323 |
| _version_ | 1797690097545510912 |
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| author | Mengyu ZHANG Jiayi PENG Jinyuan WEI Jingxian YANG Qingping ZHONG |
| author_facet | Mengyu ZHANG Jiayi PENG Jinyuan WEI Jingxian YANG Qingping ZHONG |
| author_sort | Mengyu ZHANG |
| collection | DOAJ |
| description | Foodborne pathogen is an important factor affecting food safety. With the biotechnology development and the improved requirements for food safety, the detection methods of foodborne pathogens are constantly updated and improved. By microfluidic technology, the processes of sample pretreatment, separation and detection are integrated on a tiny chip, and a variety of functions are completed. As an important branch, droplet microfluidic technology can be used for high-throughput detection with the dispersed microdroplets formed by two insoluble liquids. In this paper, according to the different ways of nucleic acid amplification, the characteristics of droplet microfluidic-digital polymerase chain reaction, droplet microfluidic-digital loop-mediated isothermal amplification, and droplet microfluidic-digital recombinase polymerase amplification are compared and summarized. The applications of these methods in the detections of foodborne pathogens are also presented. In addition, the future developments of these technologies are prospected. The intelligent, continuous, precise and miniaturized rapid detections of foodborne pathogens are realized by droplet microfluidic technology combined with digital nucleic acid amplification. This paper would provide references for the development of the technology in the field of rapid detection of foodborne pathogens. |
| first_indexed | 2024-03-12T01:54:38Z |
| format | Article |
| id | doaj.art-d4e4435e418140ac8d2778038f571634 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T01:54:38Z |
| publishDate | 2023-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-d4e4435e418140ac8d2778038f5716342023-09-08T06:41:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-09-01441848449110.13386/j.issn1002-0306.20221103232022110323-18Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne PathogensMengyu ZHANG0Jiayi PENG1Jinyuan WEI2Jingxian YANG3Qingping ZHONG4Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaFoodborne pathogen is an important factor affecting food safety. With the biotechnology development and the improved requirements for food safety, the detection methods of foodborne pathogens are constantly updated and improved. By microfluidic technology, the processes of sample pretreatment, separation and detection are integrated on a tiny chip, and a variety of functions are completed. As an important branch, droplet microfluidic technology can be used for high-throughput detection with the dispersed microdroplets formed by two insoluble liquids. In this paper, according to the different ways of nucleic acid amplification, the characteristics of droplet microfluidic-digital polymerase chain reaction, droplet microfluidic-digital loop-mediated isothermal amplification, and droplet microfluidic-digital recombinase polymerase amplification are compared and summarized. The applications of these methods in the detections of foodborne pathogens are also presented. In addition, the future developments of these technologies are prospected. The intelligent, continuous, precise and miniaturized rapid detections of foodborne pathogens are realized by droplet microfluidic technology combined with digital nucleic acid amplification. This paper would provide references for the development of the technology in the field of rapid detection of foodborne pathogens.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110323droplet microfluidic technologyfoodborne pathogennucleic acid amplificationrapid detection |
| spellingShingle | Mengyu ZHANG Jiayi PENG Jinyuan WEI Jingxian YANG Qingping ZHONG Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens Shipin gongye ke-ji droplet microfluidic technology foodborne pathogen nucleic acid amplification rapid detection |
| title | Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens |
| title_full | Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens |
| title_fullStr | Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens |
| title_full_unstemmed | Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens |
| title_short | Application of Droplet Microfluidic Technology Combined with Nucleic Acid Amplification in the Detection of Foodborne Pathogens |
| title_sort | application of droplet microfluidic technology combined with nucleic acid amplification in the detection of foodborne pathogens |
| topic | droplet microfluidic technology foodborne pathogen nucleic acid amplification rapid detection |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110323 |
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