The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage
In this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI4)-induced hepatic injury in the rat was investigated. A hepatotoxic rat model was developed by the injection of CCI4 dissolved in soybean oil (1 mL/kg/twice a week, intraperitoneal (i.p.) injection). Following the forma...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2018-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700055D.pdf |
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| author | Deniz Gulsah Yildiz Laloglu Esra Koc Kubra Nadaroglu Hayrunnisa Geyikoglu Fatime |
| author_facet | Deniz Gulsah Yildiz Laloglu Esra Koc Kubra Nadaroglu Hayrunnisa Geyikoglu Fatime |
| author_sort | Deniz Gulsah Yildiz |
| collection | DOAJ |
| description | In this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI4)-induced hepatic injury in the rat was investigated. A hepatotoxic rat model was developed by the injection of CCI4 dissolved in soybean oil (1 mL/kg/twice a week, intraperitoneal (i.p.) injection). Following the formation of hepatic injury, extracts of M. nigra at doses ranging from 150 to 300 mg/kg were administered to rats by i.p. injection for eight weeks. At the end of administration, rat livers were excised by dissection. The activities of liver enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) were detected in the serum, and the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were established in the liver. Histological changes and immunohistochemical localization of caspase-3 and 8-oxo-2’-deoxyguanosine (8-OhdG) were performed by hematoxylin-eosin (H&E) staining of liver sections and caspase-3 and 8-OhdG immunohistochemical staining. The results showed that the M. nigra extract prevented protein oxidation generated by CCI4. The extracts demonstrated the ability to modulate the activity of SOD and GPx, and also prevented the CCI4-induced increase in AST and GGT levels. These results indicate that M. nigra extracts provided significant protection against CCl4-induced hepatic injury and might also present a novel approach for the treatment of some liver diseases. |
| first_indexed | 2024-12-23T11:30:22Z |
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| id | doaj.art-d4e57b8c648942a0b910368b7f42b784 |
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| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-23T11:30:22Z |
| publishDate | 2018-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-d4e57b8c648942a0b910368b7f42b7842022-12-21T17:48:49ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392018-01-0170237137810.2298/ABS171009055D0354-46641700055DThe effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damageDeniz Gulsah Yildiz0Laloglu Esra1Koc Kubra2Nadaroglu Hayrunnisa3Geyikoglu Fatime4Ataturk University, Vocational High School of Health Services, Erzurum, TurkeyAtaturk University, Faculty of Medicine, Departments of Medical Biochemistry, Erzurum, TurkeyAtaturk University, Faculty of Science, Department of Biology, Erzurum, TurkeyAtaturk University, Erzurum Vocational Training School, Department of Food Technology, Erzurum, TurkeyAtaturk University, Faculty of Science, Department of Biology, Erzurum, TurkeyIn this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI4)-induced hepatic injury in the rat was investigated. A hepatotoxic rat model was developed by the injection of CCI4 dissolved in soybean oil (1 mL/kg/twice a week, intraperitoneal (i.p.) injection). Following the formation of hepatic injury, extracts of M. nigra at doses ranging from 150 to 300 mg/kg were administered to rats by i.p. injection for eight weeks. At the end of administration, rat livers were excised by dissection. The activities of liver enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) were detected in the serum, and the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were established in the liver. Histological changes and immunohistochemical localization of caspase-3 and 8-oxo-2’-deoxyguanosine (8-OhdG) were performed by hematoxylin-eosin (H&E) staining of liver sections and caspase-3 and 8-OhdG immunohistochemical staining. The results showed that the M. nigra extract prevented protein oxidation generated by CCI4. The extracts demonstrated the ability to modulate the activity of SOD and GPx, and also prevented the CCI4-induced increase in AST and GGT levels. These results indicate that M. nigra extracts provided significant protection against CCl4-induced hepatic injury and might also present a novel approach for the treatment of some liver diseases.http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700055D.pdfMorus nigrahepatic injuryantioxidant enzymescaspase-38-hydroxydeoxyguanosine |
| spellingShingle | Deniz Gulsah Yildiz Laloglu Esra Koc Kubra Nadaroglu Hayrunnisa Geyikoglu Fatime The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage Archives of Biological Sciences Morus nigra hepatic injury antioxidant enzymes caspase-3 8-hydroxydeoxyguanosine |
| title | The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage |
| title_full | The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage |
| title_fullStr | The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage |
| title_full_unstemmed | The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage |
| title_short | The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage |
| title_sort | effect of black mulberry morus nigra extract on carbon tetrachloride induced liver damage |
| topic | Morus nigra hepatic injury antioxidant enzymes caspase-3 8-hydroxydeoxyguanosine |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700055D.pdf |
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