Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy
Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high through...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2013-03-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174782/ |
| _version_ | 1828306887079100416 |
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| author | Saetbyeol Kim Sanguk Lee Hee-Youn Chi Mi-Kyeong Kim Jeong-Soo Kim Su-Heon Lee Hoeil Chung |
| author_facet | Saetbyeol Kim Sanguk Lee Hee-Youn Chi Mi-Kyeong Kim Jeong-Soo Kim Su-Heon Lee Hoeil Chung |
| author_sort | Saetbyeol Kim |
| collection | DOAJ |
| description | Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique. |
| first_indexed | 2024-04-13T14:50:09Z |
| format | Article |
| id | doaj.art-d4e66f53585249ab89b11ee796d15127 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-04-13T14:50:09Z |
| publishDate | 2013-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-d4e66f53585249ab89b11ee796d151272022-12-22T02:42:37ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542013-03-0129110510910.5423/PPJ.NT.09.2012.0147PPJ.NT.09.2012.0147Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman SpectroscopySaetbyeol Kim0Sanguk Lee1Hee-Youn Chi2Mi-Kyeong Kim3Jeong-Soo Kim4Su-Heon Lee5Hoeil Chung6Department of Chemistry, Hanyang University, Seoul 133-791, KoreaDepartment of Chemistry, Hanyang University, Seoul 133-791, KoreaSmarteome. Co., Ltd., Suwon 441-853, KoreaAgricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, KoreaAgricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, KoreaSchool of Applied Biosciences, College of Agriculture & Life Scieneces, Kyungpook National University, Daegu 702-701, KoreaDepartment of Chemistry, Hanyang University, Seoul 133-791, KoreaRaman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174782/discriminationRaman spectroscopysurface enhanced Raman scattering (SERS)Turnip yellow mosaic virus (TYMV)virus detection |
| spellingShingle | Saetbyeol Kim Sanguk Lee Hee-Youn Chi Mi-Kyeong Kim Jeong-Soo Kim Su-Heon Lee Hoeil Chung Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy The Plant Pathology Journal discrimination Raman spectroscopy surface enhanced Raman scattering (SERS) Turnip yellow mosaic virus (TYMV) virus detection |
| title | Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy |
| title_full | Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy |
| title_fullStr | Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy |
| title_full_unstemmed | Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy |
| title_short | Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy |
| title_sort | feasibility study for detection of turnip yellow mosaic virus tymv infection of chinese cabbage plants using raman spectroscopy |
| topic | discrimination Raman spectroscopy surface enhanced Raman scattering (SERS) Turnip yellow mosaic virus (TYMV) virus detection |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174782/ |
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