Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality,...
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Format: | Article |
Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2014-05-01
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Series: | Turkish Journal of Agriculture: Food Science and Technology |
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Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/94 |
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author | Hatice Öğütcü Ömer Faruk Algur |
author_facet | Hatice Öğütcü Ömer Faruk Algur |
author_sort | Hatice Öğütcü |
collection | DOAJ |
description | In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp. |
first_indexed | 2024-04-10T11:27:03Z |
format | Article |
id | doaj.art-d4e6eb8d1be34bf5841b8666887da5b6 |
institution | Directory Open Access Journal |
issn | 2148-127X |
language | English |
last_indexed | 2024-04-10T11:27:03Z |
publishDate | 2014-05-01 |
publisher | Turkish Science and Technology Publishing (TURSTEP) |
record_format | Article |
series | Turkish Journal of Agriculture: Food Science and Technology |
spelling | doaj.art-d4e6eb8d1be34bf5841b8666887da5b62023-02-15T16:18:22ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2014-05-012418118410.24925/turjaf.v2i4.181-184.9462Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum PlantsHatice Öğütcü0Ömer Faruk Algur1Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 40100 KırşehirAtatürk Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, 25100 ErzurumIn our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.http://www.agrifoodscience.com/index.php/TURJAF/article/view/94Medicago spp.NodülasyonMikrobiyal gübreRhizobium spp. |
spellingShingle | Hatice Öğütcü Ömer Faruk Algur Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants Turkish Journal of Agriculture: Food Science and Technology Medicago spp. Nodülasyon Mikrobiyal gübre Rhizobium spp. |
title | Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants |
title_full | Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants |
title_fullStr | Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants |
title_full_unstemmed | Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants |
title_short | Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants |
title_sort | isolation of rhizobium spp bacteria which as used microbial fertilizer from wild leguminosarum plants |
topic | Medicago spp. Nodülasyon Mikrobiyal gübre Rhizobium spp. |
url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/94 |
work_keys_str_mv | AT haticeogutcu isolationofrhizobiumsppbacteriawhichasusedmicrobialfertilizerfromwildleguminosarumplants AT omerfarukalgur isolationofrhizobiumsppbacteriawhichasusedmicrobialfertilizerfromwildleguminosarumplants |