Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants

In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality,...

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Main Authors: Hatice Öğütcü, Ömer Faruk Algur
Format: Article
Language:English
Published: Turkish Science and Technology Publishing (TURSTEP) 2014-05-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/94
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author Hatice Öğütcü
Ömer Faruk Algur
author_facet Hatice Öğütcü
Ömer Faruk Algur
author_sort Hatice Öğütcü
collection DOAJ
description In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.
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spelling doaj.art-d4e6eb8d1be34bf5841b8666887da5b62023-02-15T16:18:22ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2014-05-012418118410.24925/turjaf.v2i4.181-184.9462Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum PlantsHatice Öğütcü0Ömer Faruk Algur1Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 40100 KırşehirAtatürk Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, 25100 ErzurumIn our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.http://www.agrifoodscience.com/index.php/TURJAF/article/view/94Medicago spp.NodülasyonMikrobiyal gübreRhizobium spp.
spellingShingle Hatice Öğütcü
Ömer Faruk Algur
Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
Turkish Journal of Agriculture: Food Science and Technology
Medicago spp.
Nodülasyon
Mikrobiyal gübre
Rhizobium spp.
title Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
title_full Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
title_fullStr Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
title_full_unstemmed Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
title_short Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants
title_sort isolation of rhizobium spp bacteria which as used microbial fertilizer from wild leguminosarum plants
topic Medicago spp.
Nodülasyon
Mikrobiyal gübre
Rhizobium spp.
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/94
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AT omerfarukalgur isolationofrhizobiumsppbacteriawhichasusedmicrobialfertilizerfromwildleguminosarumplants