A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers
Aim: Colon cancer is one of the leading causes of cancer-related mortality. However, specific biomarkers for its diagnosis or treatment are not established well. Methods: We developed a colon-cancer specific peptide probe using phage display libraries. We validated the specificity of this probe to c...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Translational Oncology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523320302515 |
| _version_ | 1818283315231195136 |
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| author | Moon Hwa Kwak Gawon Yi Seung Mok Yang Younghee Choe Sangkee Choi Hye-soo Lee Eunha Kim Yong-beom Lim Kun Na Myung-Gyu Choi Heebeom Koo Jae Myung Park |
| author_facet | Moon Hwa Kwak Gawon Yi Seung Mok Yang Younghee Choe Sangkee Choi Hye-soo Lee Eunha Kim Yong-beom Lim Kun Na Myung-Gyu Choi Heebeom Koo Jae Myung Park |
| author_sort | Moon Hwa Kwak |
| collection | DOAJ |
| description | Aim: Colon cancer is one of the leading causes of cancer-related mortality. However, specific biomarkers for its diagnosis or treatment are not established well. Methods: We developed a colon-cancer specific peptide probe using phage display libraries. We validated the specificity of this probe to colon cancer cells with immunohistochemical staining and FACS analysis using one normal cell and five colon cancer cell lines. Results: This peptide probe maintained binding affinity even after serum incubation. For therapeutic applications, this peptide probe was conjugated to hematoporphyrin, a photosensitizer, which showed a significantly enhanced cellular uptake and high photodynamic effect to kill tumor cells. As another application, we made a nanoparticle modified from the peptide probe. It efficiently delivered SN-38, an anticancer drug, into tumor cells, and its tumor-targeting ability was observed in vivo after intravenous injection to the same xenograft model. Conclusion: The noble dodecapeptide probe can be a promising candidate for both colon tumor diagnosis and targeted drug delivery. |
| first_indexed | 2024-12-13T00:34:57Z |
| format | Article |
| id | doaj.art-d4e83bba1931467bb0a4fbcade73f060 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-12-13T00:34:57Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-d4e83bba1931467bb0a4fbcade73f0602022-12-22T00:05:14ZengElsevierTranslational Oncology1936-52332020-09-01139100798A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon CancersMoon Hwa Kwak0Gawon Yi1Seung Mok Yang2Younghee Choe3Sangkee Choi4Hye-soo Lee5Eunha Kim6Yong-beom Lim7Kun Na8Myung-Gyu Choi9Heebeom Koo10Jae Myung Park11Catholic Photomedicine Research Institute, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaCatholic Photomedicine Research Institute, The Catholic University of Korea, Seoul, Republic of KoreaCatholic Photomedicine Research Institute, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Materials Science and Engineering, Yonsei University, Seoul, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Materials Science and Engineering, Yonsei University, Seoul, Republic of KoreaDepartment of Biotechnology, The Catholic University of Korea, Seoul, Republic of KoreaCatholic Photomedicine Research Institute, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Address all correspondence to: Jae Myung Park, MD, Division of Gastroenterology & Hepatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-GU, Seoul 06591, Korea. or Heebeom Koo, Ph.D., Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea.Catholic Photomedicine Research Institute, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea; Address all correspondence to: Jae Myung Park, MD, Division of Gastroenterology & Hepatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-GU, Seoul 06591, Korea. or Heebeom Koo, Ph.D., Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea.Aim: Colon cancer is one of the leading causes of cancer-related mortality. However, specific biomarkers for its diagnosis or treatment are not established well. Methods: We developed a colon-cancer specific peptide probe using phage display libraries. We validated the specificity of this probe to colon cancer cells with immunohistochemical staining and FACS analysis using one normal cell and five colon cancer cell lines. Results: This peptide probe maintained binding affinity even after serum incubation. For therapeutic applications, this peptide probe was conjugated to hematoporphyrin, a photosensitizer, which showed a significantly enhanced cellular uptake and high photodynamic effect to kill tumor cells. As another application, we made a nanoparticle modified from the peptide probe. It efficiently delivered SN-38, an anticancer drug, into tumor cells, and its tumor-targeting ability was observed in vivo after intravenous injection to the same xenograft model. Conclusion: The noble dodecapeptide probe can be a promising candidate for both colon tumor diagnosis and targeted drug delivery.http://www.sciencedirect.com/science/article/pii/S1936523320302515 |
| spellingShingle | Moon Hwa Kwak Gawon Yi Seung Mok Yang Younghee Choe Sangkee Choi Hye-soo Lee Eunha Kim Yong-beom Lim Kun Na Myung-Gyu Choi Heebeom Koo Jae Myung Park A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers Translational Oncology |
| title | A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers |
| title_full | A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers |
| title_fullStr | A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers |
| title_full_unstemmed | A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers |
| title_short | A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers |
| title_sort | dodecapeptide selected by phage display as a potential theranostic probe for colon cancers |
| url | http://www.sciencedirect.com/science/article/pii/S1936523320302515 |
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