Target SNP selection in complex disease association studies
<p>Abstract</p> <p>Background</p> <p>The massive amount of SNP data stored at public internet sites provides unprecedented access to human genetic variation. Selecting target SNP for disease-gene association studies is currently done more or less randomly as decision ru...
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| Format: | Article |
| Language: | English |
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BMC
2004-07-01
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| Series: | BMC Bioinformatics |
| Online Access: | http://www.biomedcentral.com/1471-2105/5/92 |
| _version_ | 1818390298421624832 |
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| author | Wjst Matthias |
| author_facet | Wjst Matthias |
| author_sort | Wjst Matthias |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The massive amount of SNP data stored at public internet sites provides unprecedented access to human genetic variation. Selecting target SNP for disease-gene association studies is currently done more or less randomly as decision rules for the selection of functional relevant SNPs are not available.</p> <p>Results</p> <p>We implemented a computational pipeline that retrieves the genomic sequence of target genes, collects information about sequence variation and selects functional motifs containing SNPs. Motifs being considered are gene promoter, exon-intron structure, AU-rich mRNA elements, transcription factor binding motifs, cryptic and enhancer splice sites together with expression in target tissue.</p> <p>As a case study, 396 genes on chromosome 6p21 in the extended HLA region were selected that contributed nearly 20,000 SNPs. By computer annotation ~2,500 SNPs in functional motifs could be identified. Most of these SNPs are disrupting transcription factor binding sites but only those introducing new sites had a significant depressing effect on SNP allele frequency. Other decision rules concern position within motifs, the validity of SNP database entries, the unique occurrence in the genome and conserved sequence context in other mammalian genomes.</p> <p>Conclusion</p> <p>Only 10% of all gene-based SNPs have sequence-predicted functional relevance making them a primary target for genotyping in association studies.</p> |
| first_indexed | 2024-12-14T04:55:24Z |
| format | Article |
| id | doaj.art-d4e87e567e884499b3609069578a12cf |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-12-14T04:55:24Z |
| publishDate | 2004-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj.art-d4e87e567e884499b3609069578a12cf2022-12-21T23:16:23ZengBMCBMC Bioinformatics1471-21052004-07-01519210.1186/1471-2105-5-92Target SNP selection in complex disease association studiesWjst Matthias<p>Abstract</p> <p>Background</p> <p>The massive amount of SNP data stored at public internet sites provides unprecedented access to human genetic variation. Selecting target SNP for disease-gene association studies is currently done more or less randomly as decision rules for the selection of functional relevant SNPs are not available.</p> <p>Results</p> <p>We implemented a computational pipeline that retrieves the genomic sequence of target genes, collects information about sequence variation and selects functional motifs containing SNPs. Motifs being considered are gene promoter, exon-intron structure, AU-rich mRNA elements, transcription factor binding motifs, cryptic and enhancer splice sites together with expression in target tissue.</p> <p>As a case study, 396 genes on chromosome 6p21 in the extended HLA region were selected that contributed nearly 20,000 SNPs. By computer annotation ~2,500 SNPs in functional motifs could be identified. Most of these SNPs are disrupting transcription factor binding sites but only those introducing new sites had a significant depressing effect on SNP allele frequency. Other decision rules concern position within motifs, the validity of SNP database entries, the unique occurrence in the genome and conserved sequence context in other mammalian genomes.</p> <p>Conclusion</p> <p>Only 10% of all gene-based SNPs have sequence-predicted functional relevance making them a primary target for genotyping in association studies.</p>http://www.biomedcentral.com/1471-2105/5/92 |
| spellingShingle | Wjst Matthias Target SNP selection in complex disease association studies BMC Bioinformatics |
| title | Target SNP selection in complex disease association studies |
| title_full | Target SNP selection in complex disease association studies |
| title_fullStr | Target SNP selection in complex disease association studies |
| title_full_unstemmed | Target SNP selection in complex disease association studies |
| title_short | Target SNP selection in complex disease association studies |
| title_sort | target snp selection in complex disease association studies |
| url | http://www.biomedcentral.com/1471-2105/5/92 |
| work_keys_str_mv | AT wjstmatthias targetsnpselectionincomplexdiseaseassociationstudies |