Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.)
Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2023-06-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/14666 |
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| author | Valbona SOTA Donald SHUKA Shawky BEKHEET Efigjeni KONGJIKA |
| author_facet | Valbona SOTA Donald SHUKA Shawky BEKHEET Efigjeni KONGJIKA |
| author_sort | Valbona SOTA |
| collection | DOAJ |
| description | Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types of culture media, Murashige & Skoog (MS) and Woody Plant Medium (WPM), were tested, and the effects of both GA3 concentration and culture media used were evaluated. For the subculture stage, three cytokinins (6-benzylaminopurine / BAP, kinetin, zeatin) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1), were compared for the RGR index, while for the rooting stage, two different auxins (1-naphthaleneacetic acid / NAA and indole-3-butyric acid / IBA) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1) were tested. GA3 at 500 mg l-1 and MS medium resulted as more effective. The highest value of the RGR index during the subculture stage was obtained in the MS nutrient medium supplemented with BAP at 1.5 mg l-1. For rhizogenesis response, IBA was more effective for roots and length number. Based on these results, in vitro methodologies can be a promising tool for the mass production of this endangered plant species and with possible applications for enhancing the production of valuable nutraceuticals. |
| first_indexed | 2024-03-13T02:58:59Z |
| format | Article |
| id | doaj.art-d4e9de3fe5d148cdbdb1c93d4fdf71a2 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:51:24Z |
| publishDate | 2023-06-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-d4e9de3fe5d148cdbdb1c93d4fdf71a22025-03-12T12:16:59ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412023-06-01119211010.14720/aas.2023.119.2.273821058Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.)Valbona SOTA0Donald SHUKA1Shawky BEKHEET2Efigjeni KONGJIKA3Department of Biotechnology, Faculty of Natural Sciences, University of Tirana, Tirana, AlbaniaDepartment of Biology, University of Vlora “Ismail Qemali”, Vlora, AlbaniaDepartament of Plant Biotechnology, National Research Center, Cairo, EgyptAcademy of Sciences of Albania, Section of Natural and Technical Sciences, Tirana, AlbaniaIronwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types of culture media, Murashige & Skoog (MS) and Woody Plant Medium (WPM), were tested, and the effects of both GA3 concentration and culture media used were evaluated. For the subculture stage, three cytokinins (6-benzylaminopurine / BAP, kinetin, zeatin) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1), were compared for the RGR index, while for the rooting stage, two different auxins (1-naphthaleneacetic acid / NAA and indole-3-butyric acid / IBA) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1) were tested. GA3 at 500 mg l-1 and MS medium resulted as more effective. The highest value of the RGR index during the subculture stage was obtained in the MS nutrient medium supplemented with BAP at 1.5 mg l-1. For rhizogenesis response, IBA was more effective for roots and length number. Based on these results, in vitro methodologies can be a promising tool for the mass production of this endangered plant species and with possible applications for enhancing the production of valuable nutraceuticals.https://journals.uni-lj.si/aas/article/view/14666mountain teamicropropagation seed germinationnutrient mediumga3 concentration |
| spellingShingle | Valbona SOTA Donald SHUKA Shawky BEKHEET Efigjeni KONGJIKA Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) Acta Agriculturae Slovenica mountain tea micropropagation seed germination nutrient medium ga3 concentration |
| title | Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) |
| title_full | Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) |
| title_fullStr | Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) |
| title_full_unstemmed | Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) |
| title_short | Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.) |
| title_sort | establishment of an in vitro method for micropropagation of ironwort sideritis raeseri boiss heldr |
| topic | mountain tea micropropagation seed germination nutrient medium ga3 concentration |
| url | https://journals.uni-lj.si/aas/article/view/14666 |
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