Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity
Anise oil was prepared in its nanoemulsion form to facilitate the penetration of microbial walls, causing microbe mortality. The penetration occurred easily owing to the reduction in its size (nm). Nanoemulsions with different concentrations of anise oil were prepared using lecithin as an emulsifyin...
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2021-06-01
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| Series: | Polymers |
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| author | Ola A. Abu Ali Mehrez E. El-Naggar Mohamed S. Abdel-Aziz Dalia I. Saleh Mohamed. A. Abu-Saied Wael A. El-Sayed |
| author_facet | Ola A. Abu Ali Mehrez E. El-Naggar Mohamed S. Abdel-Aziz Dalia I. Saleh Mohamed. A. Abu-Saied Wael A. El-Sayed |
| author_sort | Ola A. Abu Ali |
| collection | DOAJ |
| description | Anise oil was prepared in its nanoemulsion form to facilitate the penetration of microbial walls, causing microbe mortality. The penetration occurred easily owing to the reduction in its size (nm). Nanoemulsions with different concentrations of anise oil were prepared using lecithin as an emulsifying agent with the aid of an ultra-sonification process. Their morphological and chemical properties were then characterized. The promising constituents were l-Menthone (11.22%), Gurjunene (6.78%), Geranyl acetate (4.03%), Elemene (3.93%), Geranyl tiglate (3.53%), geraniol (3.48%), linalool (0.17%) as well as camphene (0.12%). Different concentrations of prepared anise oil in micro and nanoemulsions were tested as antimicrobial agents against Gram-positive bacteria (<i>Staphylococcus aureus</i>), Gram-negative bacteria (<i>Escherichia coli</i>), yeast (<i>Candida albicans</i>) and fungi (<i>Asperigillus niger</i>). The findings illustrated that the anise oil-based nanoemulsion exhibited better results. Different biochemical and biological evaluations of anise oil nanoemulsions were conducted, including determining killing times, antioxidant activities (using three different methods), and total phenolics. A trial to estimate the mode of action of anise oil-based nanoemulsion as an antimicrobial agent against <i>S. aureus</i> and <i>C. albicans</i> was performed via studying the release of reducing sugars and protein and conducting scanning electron microscopy. |
| first_indexed | 2024-03-10T10:16:00Z |
| format | Article |
| id | doaj.art-d4ec91aae03345edb83aa56229530fdf |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T10:16:00Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d4ec91aae03345edb83aa56229530fdf2023-11-22T00:52:15ZengMDPI AGPolymers2073-43602021-06-011312200910.3390/polym13122009Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial ActivityOla A. Abu Ali0Mehrez E. El-Naggar1Mohamed S. Abdel-Aziz2Dalia I. Saleh3Mohamed. A. Abu-Saied4Wael A. El-Sayed5Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaTextile Research Division, National Research Center, Dokki, Giza 12622, EgyptGenetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza 12622, EgyptDepartment of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaPolymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City 21934, Alexandria, EgyptDepartment of Chemistry, College of Science, Qassim University, Buraidah 52571, Saudi ArabiaAnise oil was prepared in its nanoemulsion form to facilitate the penetration of microbial walls, causing microbe mortality. The penetration occurred easily owing to the reduction in its size (nm). Nanoemulsions with different concentrations of anise oil were prepared using lecithin as an emulsifying agent with the aid of an ultra-sonification process. Their morphological and chemical properties were then characterized. The promising constituents were l-Menthone (11.22%), Gurjunene (6.78%), Geranyl acetate (4.03%), Elemene (3.93%), Geranyl tiglate (3.53%), geraniol (3.48%), linalool (0.17%) as well as camphene (0.12%). Different concentrations of prepared anise oil in micro and nanoemulsions were tested as antimicrobial agents against Gram-positive bacteria (<i>Staphylococcus aureus</i>), Gram-negative bacteria (<i>Escherichia coli</i>), yeast (<i>Candida albicans</i>) and fungi (<i>Asperigillus niger</i>). The findings illustrated that the anise oil-based nanoemulsion exhibited better results. Different biochemical and biological evaluations of anise oil nanoemulsions were conducted, including determining killing times, antioxidant activities (using three different methods), and total phenolics. A trial to estimate the mode of action of anise oil-based nanoemulsion as an antimicrobial agent against <i>S. aureus</i> and <i>C. albicans</i> was performed via studying the release of reducing sugars and protein and conducting scanning electron microscopy.https://www.mdpi.com/2073-4360/13/12/2009anise oilnanoemulsionemulsifying agentantimicrobial properties |
| spellingShingle | Ola A. Abu Ali Mehrez E. El-Naggar Mohamed S. Abdel-Aziz Dalia I. Saleh Mohamed. A. Abu-Saied Wael A. El-Sayed Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity Polymers anise oil nanoemulsion emulsifying agent antimicrobial properties |
| title | Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity |
| title_full | Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity |
| title_fullStr | Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity |
| title_full_unstemmed | Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity |
| title_short | Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity |
| title_sort | facile synthesis of natural anise based nanoemulsions and their antimicrobial activity |
| topic | anise oil nanoemulsion emulsifying agent antimicrobial properties |
| url | https://www.mdpi.com/2073-4360/13/12/2009 |
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