The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco
Paeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this...
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| Format: | Article |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.810990/full |
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| author | Xiaokun Liu Xiaokun Liu Xiaokun Liu Jingjing Duan Jingjing Duan Jingjing Duan Dan Huo Dan Huo Qinqin Li Qinqin Li Qiaoyun Wang Qiaoyun Wang Yanlong Zhang Yanlong Zhang Lixin Niu Lixin Niu Jianrang Luo Jianrang Luo |
| author_facet | Xiaokun Liu Xiaokun Liu Xiaokun Liu Jingjing Duan Jingjing Duan Jingjing Duan Dan Huo Dan Huo Qinqin Li Qinqin Li Qiaoyun Wang Qiaoyun Wang Yanlong Zhang Yanlong Zhang Lixin Niu Lixin Niu Jianrang Luo Jianrang Luo |
| author_sort | Xiaokun Liu |
| collection | DOAJ |
| description | Paeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this study, an R2R3-MYB, PqMYB113 was identified from the leaves of P. qiui. Phylogenetic analysis revealed that PqMYB113 clustered with Liquidambar LfMYB113 and grape VvMYBA6. Subcellular location analysis showed that PqMYB113 was located in the cell nucleus. The transient reporter assay suggested that PqMYB113 was a transcriptional activator. The overexpression of PqMYB113 in Arabidopsis thaliana and tobacco (Nicotiana tabacum) resulted in increased anthocyanin accumulation and the upregulation of CHS, F3H, F3’H, DFR, and ANS. The dual luciferase reporter assay showed that PqMYB113 could activate the promoters of PqDFR and PqANS. Bimolecular fluorescence complementation assays and yeast two-hybrid assays suggested that PqMYB113 could form a ternary MBW complex with PqbHLH1 and PqWD40 cofactors. These results provide insight into the regulation of anthocyanin biosynthesis in tree peony leaves. |
| first_indexed | 2024-04-11T17:06:17Z |
| format | Article |
| id | doaj.art-d4ef69c95613441fba14c8e1bc6c9c7d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T17:06:17Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d4ef69c95613441fba14c8e1bc6c9c7d2022-12-22T04:13:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-01-011210.3389/fpls.2021.810990810990The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and TobaccoXiaokun Liu0Xiaokun Liu1Xiaokun Liu2Jingjing Duan3Jingjing Duan4Jingjing Duan5Dan Huo6Dan Huo7Qinqin Li8Qinqin Li9Qiaoyun Wang10Qiaoyun Wang11Yanlong Zhang12Yanlong Zhang13Lixin Niu14Lixin Niu15Jianrang Luo16Jianrang Luo17College of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resource, Shanghai, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Plant Science, Tarim University, Alar, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Xianyang, ChinaNational Engineering Research Center for Oil Peony, Yangling, ChinaPaeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this study, an R2R3-MYB, PqMYB113 was identified from the leaves of P. qiui. Phylogenetic analysis revealed that PqMYB113 clustered with Liquidambar LfMYB113 and grape VvMYBA6. Subcellular location analysis showed that PqMYB113 was located in the cell nucleus. The transient reporter assay suggested that PqMYB113 was a transcriptional activator. The overexpression of PqMYB113 in Arabidopsis thaliana and tobacco (Nicotiana tabacum) resulted in increased anthocyanin accumulation and the upregulation of CHS, F3H, F3’H, DFR, and ANS. The dual luciferase reporter assay showed that PqMYB113 could activate the promoters of PqDFR and PqANS. Bimolecular fluorescence complementation assays and yeast two-hybrid assays suggested that PqMYB113 could form a ternary MBW complex with PqbHLH1 and PqWD40 cofactors. These results provide insight into the regulation of anthocyanin biosynthesis in tree peony leaves.https://www.frontiersin.org/articles/10.3389/fpls.2021.810990/fullPaeonia qiuianthocyanintranscription factorMYBpositive regulation |
| spellingShingle | Xiaokun Liu Xiaokun Liu Xiaokun Liu Jingjing Duan Jingjing Duan Jingjing Duan Dan Huo Dan Huo Qinqin Li Qinqin Li Qiaoyun Wang Qiaoyun Wang Yanlong Zhang Yanlong Zhang Lixin Niu Lixin Niu Jianrang Luo Jianrang Luo The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco Frontiers in Plant Science Paeonia qiui anthocyanin transcription factor MYB positive regulation |
| title | The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco |
| title_full | The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco |
| title_fullStr | The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco |
| title_full_unstemmed | The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco |
| title_short | The Paeonia qiui R2R3-MYB Transcription Factor PqMYB113 Positively Regulates Anthocyanin Accumulation in Arabidopsis thaliana and Tobacco |
| title_sort | paeonia qiui r2r3 myb transcription factor pqmyb113 positively regulates anthocyanin accumulation in arabidopsis thaliana and tobacco |
| topic | Paeonia qiui anthocyanin transcription factor MYB positive regulation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.810990/full |
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