Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors
In this study, an electrochemical sensor was developed by immobilizing colon cancer and the adjacent tissues (peripheral healthy tissues on both sides of the tumor) and was used to investigate the receptor sensing kinetics of glucose, sodium glutamate, disodium inosinate, and sodium lactate. The res...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1453 |
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| author | Dingqiang Lu Danyang Liu Yujiao Liu Xinqian Wang Yixuan Liu Shuai Yuan Ruijuan Ren Guangchang Pang |
| author_facet | Dingqiang Lu Danyang Liu Yujiao Liu Xinqian Wang Yixuan Liu Shuai Yuan Ruijuan Ren Guangchang Pang |
| author_sort | Dingqiang Lu |
| collection | DOAJ |
| description | In this study, an electrochemical sensor was developed by immobilizing colon cancer and the adjacent tissues (peripheral healthy tissues on both sides of the tumor) and was used to investigate the receptor sensing kinetics of glucose, sodium glutamate, disodium inosinate, and sodium lactate. The results showed that the electrical signal triggered by the ligand–receptor interaction presented hyperbolic kinetic characteristics similar to the interaction of an enzyme with its substrate. The results indicated that the activation constant values of the colon cancer tissue and adjacent tissues differed by two orders of magnitude for glucose and sodium glutamate and around one order of magnitude for disodium inosinate. The cancer tissues did not sense sodium lactate, whereas the adjacent tissues could sense sodium lactate. Compared with normal cells, cancer cells have significantly improved nutritional sensing ability, and the improvement of cancer cells’ sensing ability mainly depends on the cascade amplification of intracellular signals. However, unlike tumor-adjacent tissues, colon cancer cells lose the ability to sense lactate. This provides key evidence for the Warburg effect of cancer cells. The methods and results in this study are expected to provide a new way for cancer research, treatment, the screening of anticancer drugs, and clinical diagnoses. |
| first_indexed | 2024-03-11T09:32:48Z |
| format | Article |
| id | doaj.art-d4f08bb814534846aedd06c88b9111bd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:32:48Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d4f08bb814534846aedd06c88b9111bd2023-11-16T17:32:41ZengMDPI AGMolecules1420-30492023-02-01283145310.3390/molecules28031453Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical BiosensorsDingqiang Lu0Danyang Liu1Yujiao Liu2Xinqian Wang3Yixuan Liu4Shuai Yuan5Ruijuan Ren6Guangchang Pang7College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTianjin Institute for Food Safety Inspection Technology, Tianjin 300134, ChinaCollege of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaIn this study, an electrochemical sensor was developed by immobilizing colon cancer and the adjacent tissues (peripheral healthy tissues on both sides of the tumor) and was used to investigate the receptor sensing kinetics of glucose, sodium glutamate, disodium inosinate, and sodium lactate. The results showed that the electrical signal triggered by the ligand–receptor interaction presented hyperbolic kinetic characteristics similar to the interaction of an enzyme with its substrate. The results indicated that the activation constant values of the colon cancer tissue and adjacent tissues differed by two orders of magnitude for glucose and sodium glutamate and around one order of magnitude for disodium inosinate. The cancer tissues did not sense sodium lactate, whereas the adjacent tissues could sense sodium lactate. Compared with normal cells, cancer cells have significantly improved nutritional sensing ability, and the improvement of cancer cells’ sensing ability mainly depends on the cascade amplification of intracellular signals. However, unlike tumor-adjacent tissues, colon cancer cells lose the ability to sense lactate. This provides key evidence for the Warburg effect of cancer cells. The methods and results in this study are expected to provide a new way for cancer research, treatment, the screening of anticancer drugs, and clinical diagnoses.https://www.mdpi.com/1420-3049/28/3/1453carbon and nitrogen nutrientselectrochemical biosensoractivation constantnutrient sensingsignal amplification factor |
| spellingShingle | Dingqiang Lu Danyang Liu Yujiao Liu Xinqian Wang Yixuan Liu Shuai Yuan Ruijuan Ren Guangchang Pang Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors Molecules carbon and nitrogen nutrients electrochemical biosensor activation constant nutrient sensing signal amplification factor |
| title | Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors |
| title_full | Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors |
| title_fullStr | Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors |
| title_full_unstemmed | Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors |
| title_short | Comparative Study on the Sensing Kinetics of Carbon and Nitrogen Nutrients in Cancer Tissues and Normal Tissues Based Electrochemical Biosensors |
| title_sort | comparative study on the sensing kinetics of carbon and nitrogen nutrients in cancer tissues and normal tissues based electrochemical biosensors |
| topic | carbon and nitrogen nutrients electrochemical biosensor activation constant nutrient sensing signal amplification factor |
| url | https://www.mdpi.com/1420-3049/28/3/1453 |
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