Effective removal of staphylococcal biofilms by the endolysin LysH5.
Staphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4159335?pdf=render |
| _version_ | 1818451215196880896 |
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| author | Diana Gutiérrez Patricia Ruas-Madiedo Beatriz Martínez Ana Rodríguez Pilar García |
| author_facet | Diana Gutiérrez Patricia Ruas-Madiedo Beatriz Martínez Ana Rodríguez Pilar García |
| author_sort | Diana Gutiérrez |
| collection | DOAJ |
| description | Staphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence of persister cells, a small bacterial subpopulation that exhibits multidrug tolerance. Phage lytic enzymes have demonstrated antimicrobial activity against planktonic and sessile bacteria. However, their ability to lyse and/or select persister cells remains largely unexplored so far. In this work, the lytic activity of the endolysin LysH5 against Staphylococcus aureus and Staphylococcus epidermidis biofilms was confirmed. LysH5 reduced staphylococcal sessile cell counts by 1-3 log units, compared with the untreated control, and sub-inhibitory concentrations of this protein did not induce biofilm formation. LysH5-surviving cells were not resistant to the lytic activity of this protein, suggesting that no persister cells were selected. Moreover, to prove the lytic ability of LysH5 against this subpopulation, both S. aureus exponential cultures and persister cells obtained after treatment with rifampicin and ciprofloxacin were subsequently treated with LysH5. The results demonstrated that besides the notable activity of endolysin LysH5 against staphylococcal biofilms, persister cells were also inhibited, which raises new opportunities as an adjuvant for some antibiotics. |
| first_indexed | 2024-12-14T21:03:39Z |
| format | Article |
| id | doaj.art-d4f219bf09a348858ed221393ad31629 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T21:03:39Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d4f219bf09a348858ed221393ad316292022-12-21T22:47:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10730710.1371/journal.pone.0107307Effective removal of staphylococcal biofilms by the endolysin LysH5.Diana GutiérrezPatricia Ruas-MadiedoBeatriz MartínezAna RodríguezPilar GarcíaStaphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence of persister cells, a small bacterial subpopulation that exhibits multidrug tolerance. Phage lytic enzymes have demonstrated antimicrobial activity against planktonic and sessile bacteria. However, their ability to lyse and/or select persister cells remains largely unexplored so far. In this work, the lytic activity of the endolysin LysH5 against Staphylococcus aureus and Staphylococcus epidermidis biofilms was confirmed. LysH5 reduced staphylococcal sessile cell counts by 1-3 log units, compared with the untreated control, and sub-inhibitory concentrations of this protein did not induce biofilm formation. LysH5-surviving cells were not resistant to the lytic activity of this protein, suggesting that no persister cells were selected. Moreover, to prove the lytic ability of LysH5 against this subpopulation, both S. aureus exponential cultures and persister cells obtained after treatment with rifampicin and ciprofloxacin were subsequently treated with LysH5. The results demonstrated that besides the notable activity of endolysin LysH5 against staphylococcal biofilms, persister cells were also inhibited, which raises new opportunities as an adjuvant for some antibiotics.http://europepmc.org/articles/PMC4159335?pdf=render |
| spellingShingle | Diana Gutiérrez Patricia Ruas-Madiedo Beatriz Martínez Ana Rodríguez Pilar García Effective removal of staphylococcal biofilms by the endolysin LysH5. PLoS ONE |
| title | Effective removal of staphylococcal biofilms by the endolysin LysH5. |
| title_full | Effective removal of staphylococcal biofilms by the endolysin LysH5. |
| title_fullStr | Effective removal of staphylococcal biofilms by the endolysin LysH5. |
| title_full_unstemmed | Effective removal of staphylococcal biofilms by the endolysin LysH5. |
| title_short | Effective removal of staphylococcal biofilms by the endolysin LysH5. |
| title_sort | effective removal of staphylococcal biofilms by the endolysin lysh5 |
| url | http://europepmc.org/articles/PMC4159335?pdf=render |
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