MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles
The procoagulant effect of microparticles (MPs) contributes to hypercoagulability-induced thrombosis. We provide preliminary findings of the MPs-Activated Clotting Time (MPs-ACT) assay to determine the procoagulant activity of MPs. MPs-rich plasma was obtained and recalcified. Changes in plasma visc...
Main Authors: | , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
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SAGE Publishing
2023-02-01
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Series: | Clinical and Applied Thrombosis/Hemostasis |
Online Access: | https://doi.org/10.1177/10760296231159374 |
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author | Yalong Gao PhD Xiaotian Li PhD Yafei Qin MD Jianlong Men PhD Jing Ren PhD Xiaochun Li MM Chunlei Xu MD Qifeng Li PhD Ying Li PhD Weiyun Cui MM Shu Zhang PhD Lei Li MM Yaohua Li MD Jianning Zhang MD Li Liu PhD |
author_facet | Yalong Gao PhD Xiaotian Li PhD Yafei Qin MD Jianlong Men PhD Jing Ren PhD Xiaochun Li MM Chunlei Xu MD Qifeng Li PhD Ying Li PhD Weiyun Cui MM Shu Zhang PhD Lei Li MM Yaohua Li MD Jianning Zhang MD Li Liu PhD |
author_sort | Yalong Gao PhD |
collection | DOAJ |
description | The procoagulant effect of microparticles (MPs) contributes to hypercoagulability-induced thrombosis. We provide preliminary findings of the MPs-Activated Clotting Time (MPs-ACT) assay to determine the procoagulant activity of MPs. MPs-rich plasma was obtained and recalcified. Changes in plasma viscoelasticity were evaluated and the time to the peak viscoelastic changes was defined as the MPs-ACT. MPs concentration was measured by flow cytometry. Coagulation products produced during plasma clotting were identified by fibrin and fibrinopeptide A. MPs were prepared in vitro and added to standard plasma to simulate pathological samples. In addition, reproducibility and sensitivity were evaluated. We confirmed the linear relationship between MPs-ACT and MP concentrations. Dynamic changes in fibrin production were depicted. We simulated the correlation between MPs-ACT and standard plasma containing MPs prepared in vitro. The reproducibility of high-value and low-value samples was 6.0% and 10.8%, respectively. MPs-ACT sensitively detected hypercoagulable samples from patients with pre-eclampsia, hip fractures, and lung tumors. MPs-ACT largely reflects the procoagulant effect of MPs. MPs-ACT sensitively and rapidly detects hypercoagulability with MPs-rich plasma. It may be promising for the diagnosis of hypercoagulable states induced by MPs. |
first_indexed | 2024-04-10T07:04:20Z |
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id | doaj.art-d4f2c8f806704547893212677b89e76d |
institution | Directory Open Access Journal |
issn | 1938-2723 |
language | English |
last_indexed | 2024-04-10T07:04:20Z |
publishDate | 2023-02-01 |
publisher | SAGE Publishing |
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series | Clinical and Applied Thrombosis/Hemostasis |
spelling | doaj.art-d4f2c8f806704547893212677b89e76d2023-02-27T10:35:01ZengSAGE PublishingClinical and Applied Thrombosis/Hemostasis1938-27232023-02-012910.1177/10760296231159374MPs-ACT, an Assay to Evaluate the Procoagulant Activity of MicroparticlesYalong Gao PhD0Xiaotian Li PhD1Yafei Qin MD2Jianlong Men PhD3Jing Ren PhD4Xiaochun Li MM5Chunlei Xu MD6Qifeng Li PhD7Ying Li PhD8Weiyun Cui MM9Shu Zhang PhD10Lei Li MM11Yaohua Li MD12Jianning Zhang MD13Li Liu PhD14 Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Department of General Surgery, , Tianjin, China Precision Medicine Center, , Tianjin, China Precision Medicine Center, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, China Key Laboratory of Post-Neurotrauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education and Tianjin Neurological Institute, , Tianjin, ChinaThe procoagulant effect of microparticles (MPs) contributes to hypercoagulability-induced thrombosis. We provide preliminary findings of the MPs-Activated Clotting Time (MPs-ACT) assay to determine the procoagulant activity of MPs. MPs-rich plasma was obtained and recalcified. Changes in plasma viscoelasticity were evaluated and the time to the peak viscoelastic changes was defined as the MPs-ACT. MPs concentration was measured by flow cytometry. Coagulation products produced during plasma clotting were identified by fibrin and fibrinopeptide A. MPs were prepared in vitro and added to standard plasma to simulate pathological samples. In addition, reproducibility and sensitivity were evaluated. We confirmed the linear relationship between MPs-ACT and MP concentrations. Dynamic changes in fibrin production were depicted. We simulated the correlation between MPs-ACT and standard plasma containing MPs prepared in vitro. The reproducibility of high-value and low-value samples was 6.0% and 10.8%, respectively. MPs-ACT sensitively detected hypercoagulable samples from patients with pre-eclampsia, hip fractures, and lung tumors. MPs-ACT largely reflects the procoagulant effect of MPs. MPs-ACT sensitively and rapidly detects hypercoagulability with MPs-rich plasma. It may be promising for the diagnosis of hypercoagulable states induced by MPs.https://doi.org/10.1177/10760296231159374 |
spellingShingle | Yalong Gao PhD Xiaotian Li PhD Yafei Qin MD Jianlong Men PhD Jing Ren PhD Xiaochun Li MM Chunlei Xu MD Qifeng Li PhD Ying Li PhD Weiyun Cui MM Shu Zhang PhD Lei Li MM Yaohua Li MD Jianning Zhang MD Li Liu PhD MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles Clinical and Applied Thrombosis/Hemostasis |
title | MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles |
title_full | MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles |
title_fullStr | MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles |
title_full_unstemmed | MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles |
title_short | MPs-ACT, an Assay to Evaluate the Procoagulant Activity of Microparticles |
title_sort | mps act an assay to evaluate the procoagulant activity of microparticles |
url | https://doi.org/10.1177/10760296231159374 |
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