Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline
The prevention, prognosis and resolution of decompression sickness (DCS) are not satisfactory. The etiology of DCS has highlighted thrombotic and inflammatory phenomena that could cause severe neurological disorders or even death. Given the immunomodulatory effects described for minocycline, an anti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00933/full |
| _version_ | 1828850564116512768 |
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| author | Anne-Virginie Desruelle Pierre Louge Simone Richard Jean-Eric Blatteau Jean-Eric Blatteau Sandrine Gaillard Sébastien De Maistre Hélène David Jean-Jacques Risso Nicolas Vallée |
| author_facet | Anne-Virginie Desruelle Pierre Louge Simone Richard Jean-Eric Blatteau Jean-Eric Blatteau Sandrine Gaillard Sébastien De Maistre Hélène David Jean-Jacques Risso Nicolas Vallée |
| author_sort | Anne-Virginie Desruelle |
| collection | DOAJ |
| description | The prevention, prognosis and resolution of decompression sickness (DCS) are not satisfactory. The etiology of DCS has highlighted thrombotic and inflammatory phenomena that could cause severe neurological disorders or even death. Given the immunomodulatory effects described for minocycline, an antibiotic in widespread use, we have decided to explore its effects in an experimental model for decompression sickness. 40 control mice (Ctrl) and 40 mice treated orally with 90 mg/kg of minocycline (MINO) were subjected to a protocol in a hyperbaric chamber, compressed with air. The purpose was to mimic a scuba dive to a depth of 90 msw and its pathogenic decompression phase. Clinical examinations and blood counts were conducted after the return to the surface. For the first time they were completed by a simple infrared (IR) imaging technique in order to assess feasibility and its clinical advantage in differentiating the sick mice (DCS) from the healthy mice (NoDCS). In this tudy, exposure to the hyperbaric protocol provoked a reduction in the number of circulating leukocytes. DCS in mice, manifesting itself by paralysis or convulsion for example, is also associated with a fall in platelets count. Cold areas ( < 25°C) were detected by IR in the hind paws and tail with significant differences (p < 0.05) between DCS and NoDCS. Severe hypothermia was also shown in the DCS mice. The ROC analysis of the thermograms has made it possible to determine that an average tail temperature below 27.5°C allows us to consider the animals to be suffering from DCS (OR = 8; AUC = 0.754, p = 0.0018). Minocycline modulates blood analysis and it seems to limit the mobilization of monocytes and granulocytes after the provocative dive. While a higher proportion of mice treated with minocycline experienced DCS symptoms, there is no significant difference. The infrared imaging has made it possible to show severe hypothermia. It suggests an modification of thermregulation in DCS animals. Surveillance by infrared camera is fast and it can aid the prognosis in the case of decompression sickness in mice. |
| first_indexed | 2024-12-12T23:14:34Z |
| format | Article |
| id | doaj.art-d4f2ef0072564fc0bbf5418ff2fd3aef |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T23:14:34Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d4f2ef0072564fc0bbf5418ff2fd3aef2022-12-22T00:08:31ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-07-011010.3389/fphys.2019.00933466375Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing MinocyclineAnne-Virginie Desruelle0Pierre Louge1Simone Richard2Jean-Eric Blatteau3Jean-Eric Blatteau4Sandrine Gaillard5Sébastien De Maistre6Hélène David7Jean-Jacques Risso8Nicolas Vallée9Unité Environnements Extrêmes, Département Environnement Opérationnel, Institut de Recherche Biomédicale des Armées, Equipe Résidante de Recherche Subaquatique Opérationnelle, Toulon, FranceService de Médecine Hyperbare et Expertise Plongée, Hôpital d’Instruction des Armées, Toulon, FranceBiotech Service, Université de Toulon, Toulon, FranceUnité Environnements Extrêmes, Département Environnement Opérationnel, Institut de Recherche Biomédicale des Armées, Equipe Résidante de Recherche Subaquatique Opérationnelle, Toulon, FranceService de Médecine Hyperbare et Expertise Plongée, Hôpital d’Instruction des Armées, Toulon, FranceBiotech Service, Université de Toulon, Toulon, FranceService de Médecine Hyperbare et Expertise Plongée, Hôpital d’Instruction des Armées, Toulon, FranceApricot Inhalotherapeutics, Saint-Laurent-de-l’Île-d’Orléans, QC, CanadaUnité Environnements Extrêmes, Département Environnement Opérationnel, Institut de Recherche Biomédicale des Armées, Equipe Résidante de Recherche Subaquatique Opérationnelle, Toulon, FranceUnité Environnements Extrêmes, Département Environnement Opérationnel, Institut de Recherche Biomédicale des Armées, Equipe Résidante de Recherche Subaquatique Opérationnelle, Toulon, FranceThe prevention, prognosis and resolution of decompression sickness (DCS) are not satisfactory. The etiology of DCS has highlighted thrombotic and inflammatory phenomena that could cause severe neurological disorders or even death. Given the immunomodulatory effects described for minocycline, an antibiotic in widespread use, we have decided to explore its effects in an experimental model for decompression sickness. 40 control mice (Ctrl) and 40 mice treated orally with 90 mg/kg of minocycline (MINO) were subjected to a protocol in a hyperbaric chamber, compressed with air. The purpose was to mimic a scuba dive to a depth of 90 msw and its pathogenic decompression phase. Clinical examinations and blood counts were conducted after the return to the surface. For the first time they were completed by a simple infrared (IR) imaging technique in order to assess feasibility and its clinical advantage in differentiating the sick mice (DCS) from the healthy mice (NoDCS). In this tudy, exposure to the hyperbaric protocol provoked a reduction in the number of circulating leukocytes. DCS in mice, manifesting itself by paralysis or convulsion for example, is also associated with a fall in platelets count. Cold areas ( < 25°C) were detected by IR in the hind paws and tail with significant differences (p < 0.05) between DCS and NoDCS. Severe hypothermia was also shown in the DCS mice. The ROC analysis of the thermograms has made it possible to determine that an average tail temperature below 27.5°C allows us to consider the animals to be suffering from DCS (OR = 8; AUC = 0.754, p = 0.0018). Minocycline modulates blood analysis and it seems to limit the mobilization of monocytes and granulocytes after the provocative dive. While a higher proportion of mice treated with minocycline experienced DCS symptoms, there is no significant difference. The infrared imaging has made it possible to show severe hypothermia. It suggests an modification of thermregulation in DCS animals. Surveillance by infrared camera is fast and it can aid the prognosis in the case of decompression sickness in mice.https://www.frontiersin.org/article/10.3389/fphys.2019.00933/fullischemiabubblediveneuroprotectioncapillary leaktetracycline |
| spellingShingle | Anne-Virginie Desruelle Pierre Louge Simone Richard Jean-Eric Blatteau Jean-Eric Blatteau Sandrine Gaillard Sébastien De Maistre Hélène David Jean-Jacques Risso Nicolas Vallée Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline Frontiers in Physiology ischemia bubble dive neuroprotection capillary leak tetracycline |
| title | Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline |
| title_full | Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline |
| title_fullStr | Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline |
| title_full_unstemmed | Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline |
| title_short | Demonstration by Infra-Red Imaging of a Temperature Control Defect in a Decompression Sickness Model Testing Minocycline |
| title_sort | demonstration by infra red imaging of a temperature control defect in a decompression sickness model testing minocycline |
| topic | ischemia bubble dive neuroprotection capillary leak tetracycline |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00933/full |
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