Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe
We reported on uric acid (UA) detection using a new fluorescence-based assay: poly-(vinylpyrrolidone) templated copper nanoclusters (PVP-CuNCs) with uricase in an aqueous medium, such as human urine with uricase. These nanoclusters were synthesized in a simple wet chemical method and their morpholog...
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MDPI AG
2023-05-01
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| Series: | Chemosensors |
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| author | Ramar Rajamanikandan Malaichamy Ilanchelian Heongkyu Ju |
| author_facet | Ramar Rajamanikandan Malaichamy Ilanchelian Heongkyu Ju |
| author_sort | Ramar Rajamanikandan |
| collection | DOAJ |
| description | We reported on uric acid (UA) detection using a new fluorescence-based assay: poly-(vinylpyrrolidone) templated copper nanoclusters (PVP-CuNCs) with uricase in an aqueous medium, such as human urine with uricase. These nanoclusters were synthesized in a simple wet chemical method and their morphological and optical properties were examined with the aid of high-resolution transmission electron microscopy and optical absorbance/emission spectroscopy. The PVP-CuNCs acted as the fluorescence indicators that used the enzyme-catalyzed oxidation of UA with uricase. Adding UA into the hybrid PVP-CuNCs/uricase solution caused enzyme-catalyzed oxidation to occur, producing hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), allantoin, and carbon dioxide. The fluorescence intensity of PVP-CuNCs is decreased by this biocatalytically generated H<sub>2</sub>O<sub>2</sub>, and this decrease is proportional to the UA level. A calibration plot showed the linear relationship with the negative slope between fluorescence intensity and UA in the range of 5–100 × 10<sup>−7</sup> mol/L. The limit of detection (LOD) of UA was estimated as 113 × 10<sup>−9</sup> mol/L. This fluorescent probe turned out to be highly specific for UA over other biologically relevant molecules. The demonstrated capability of the PVP-CuNCs as the nanoprobes for quantification of the UA levels in human urine samples could potentially pave the way toward medical applications where a super-sensitive, cost-effective, and UA-specific diagnosis was required. |
| first_indexed | 2024-03-11T03:51:09Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T03:51:09Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Chemosensors |
| spelling | doaj.art-d4f5c871aa7b4c4cbd68884750ca04b72023-11-18T00:53:52ZengMDPI AGChemosensors2227-90402023-05-0111526810.3390/chemosensors11050268Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence ProbeRamar Rajamanikandan0Malaichamy Ilanchelian1Heongkyu Ju2Department of Physics, Gachon University, Seongnam-si 13120, Republic of KoreaDepartment of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, IndiaDepartment of Physics, Gachon University, Seongnam-si 13120, Republic of KoreaWe reported on uric acid (UA) detection using a new fluorescence-based assay: poly-(vinylpyrrolidone) templated copper nanoclusters (PVP-CuNCs) with uricase in an aqueous medium, such as human urine with uricase. These nanoclusters were synthesized in a simple wet chemical method and their morphological and optical properties were examined with the aid of high-resolution transmission electron microscopy and optical absorbance/emission spectroscopy. The PVP-CuNCs acted as the fluorescence indicators that used the enzyme-catalyzed oxidation of UA with uricase. Adding UA into the hybrid PVP-CuNCs/uricase solution caused enzyme-catalyzed oxidation to occur, producing hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), allantoin, and carbon dioxide. The fluorescence intensity of PVP-CuNCs is decreased by this biocatalytically generated H<sub>2</sub>O<sub>2</sub>, and this decrease is proportional to the UA level. A calibration plot showed the linear relationship with the negative slope between fluorescence intensity and UA in the range of 5–100 × 10<sup>−7</sup> mol/L. The limit of detection (LOD) of UA was estimated as 113 × 10<sup>−9</sup> mol/L. This fluorescent probe turned out to be highly specific for UA over other biologically relevant molecules. The demonstrated capability of the PVP-CuNCs as the nanoprobes for quantification of the UA levels in human urine samples could potentially pave the way toward medical applications where a super-sensitive, cost-effective, and UA-specific diagnosis was required.https://www.mdpi.com/2227-9040/11/5/268copper nanoclustershuman urinecatalytic oxidationuric acid detectionuricasefluorescence |
| spellingShingle | Ramar Rajamanikandan Malaichamy Ilanchelian Heongkyu Ju Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe Chemosensors copper nanoclusters human urine catalytic oxidation uric acid detection uricase fluorescence |
| title | Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe |
| title_full | Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe |
| title_fullStr | Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe |
| title_full_unstemmed | Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe |
| title_short | Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe |
| title_sort | highly selective uricase based quantification of uric acid using hydrogen peroxide sensitive poly vinylpyrrolidone templated copper nanoclusters as a fluorescence probe |
| topic | copper nanoclusters human urine catalytic oxidation uric acid detection uricase fluorescence |
| url | https://www.mdpi.com/2227-9040/11/5/268 |
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