In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts
We hypothesized that the neonatal rat heart would bring transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to maturity as it grows to adult size. In neonatal rat heart, engrafted hiPSC derivatives developed partially matured myofibrils after 3 months, with increasing...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671116302478 |
| _version_ | 1811310262681075712 |
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| author | Shin Kadota Lil Pabon Hans Reinecke Charles E. Murry |
| author_facet | Shin Kadota Lil Pabon Hans Reinecke Charles E. Murry |
| author_sort | Shin Kadota |
| collection | DOAJ |
| description | We hypothesized that the neonatal rat heart would bring transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to maturity as it grows to adult size. In neonatal rat heart, engrafted hiPSC derivatives developed partially matured myofibrils after 3 months, with increasing cell size and sarcomere length. There was no difference between grafts from hiPSC-CMs or hiPSC-derived cardiac progenitors (hiPSC-CPs) at 3 months, nor was maturation influenced by infarction. Interestingly, the infarcted adult heart induced greater human cardiomyocyte hypertrophy and induction of cardiac troponin I expression than the neonatal heart. Although human cardiomyocytes at all time points were significantly smaller than the host rat cardiomyocytes, transplanted neonatal rat cardiomyocytes reached adult size and structure by 3 months. Thus, the adult rat heart induces faster maturation than the neonatal heart, and human cardiomyocytes mature more slowly than rat cardiomyocytes. The slower maturation of human cardiomyocytes could be related to environmental mismatch or cell-autonomous factors. |
| first_indexed | 2024-04-13T09:56:06Z |
| format | Article |
| id | doaj.art-d4f6075a460d4ab68a2943a6c93abd82 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-13T09:56:06Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-d4f6075a460d4ab68a2943a6c93abd822022-12-22T02:51:26ZengElsevierStem Cell Reports2213-67112017-02-018227828910.1016/j.stemcr.2016.10.009In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat HeartsShin Kadota0Lil Pabon1Hans Reinecke2Charles E. Murry3Department of Pathology, University of Washington, 850 Republican Street, Brotman Building Room 453, Seattle, WA 98109, USADepartment of Pathology, University of Washington, 850 Republican Street, Brotman Building Room 453, Seattle, WA 98109, USADepartment of Pathology, University of Washington, 850 Republican Street, Brotman Building Room 453, Seattle, WA 98109, USADepartment of Pathology, University of Washington, 850 Republican Street, Brotman Building Room 453, Seattle, WA 98109, USAWe hypothesized that the neonatal rat heart would bring transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to maturity as it grows to adult size. In neonatal rat heart, engrafted hiPSC derivatives developed partially matured myofibrils after 3 months, with increasing cell size and sarcomere length. There was no difference between grafts from hiPSC-CMs or hiPSC-derived cardiac progenitors (hiPSC-CPs) at 3 months, nor was maturation influenced by infarction. Interestingly, the infarcted adult heart induced greater human cardiomyocyte hypertrophy and induction of cardiac troponin I expression than the neonatal heart. Although human cardiomyocytes at all time points were significantly smaller than the host rat cardiomyocytes, transplanted neonatal rat cardiomyocytes reached adult size and structure by 3 months. Thus, the adult rat heart induces faster maturation than the neonatal heart, and human cardiomyocytes mature more slowly than rat cardiomyocytes. The slower maturation of human cardiomyocytes could be related to environmental mismatch or cell-autonomous factors.http://www.sciencedirect.com/science/article/pii/S2213671116302478maturationhuman pluripotent stem cell-derived cardiomyocyteneonatal rat |
| spellingShingle | Shin Kadota Lil Pabon Hans Reinecke Charles E. Murry In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts Stem Cell Reports maturation human pluripotent stem cell-derived cardiomyocyte neonatal rat |
| title | In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts |
| title_full | In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts |
| title_fullStr | In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts |
| title_full_unstemmed | In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts |
| title_short | In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts |
| title_sort | in vivo maturation of human induced pluripotent stem cell derived cardiomyocytes in neonatal and adult rat hearts |
| topic | maturation human pluripotent stem cell-derived cardiomyocyte neonatal rat |
| url | http://www.sciencedirect.com/science/article/pii/S2213671116302478 |
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