A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents
Abstract Background Bioplastics are attracting considerable attention, owing to the increase in non-degradable waste. Using microorganisms to degrade bioplastics is a promising strategy for reducing non-degradable plastic waste. However, maintaining bacterial viability and activity during culture an...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02173-4 |
| _version_ | 1797451010300444672 |
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| author | Su Hyun Kim Nara Shin Suk Jin Oh Jeong Hyeon Hwang Hyun Jin Kim Shashi Kant Bhatia Jeonghee Yun Jae-Seok Kim Yung-Hun Yang |
| author_facet | Su Hyun Kim Nara Shin Suk Jin Oh Jeong Hyeon Hwang Hyun Jin Kim Shashi Kant Bhatia Jeonghee Yun Jae-Seok Kim Yung-Hun Yang |
| author_sort | Su Hyun Kim |
| collection | DOAJ |
| description | Abstract Background Bioplastics are attracting considerable attention, owing to the increase in non-degradable waste. Using microorganisms to degrade bioplastics is a promising strategy for reducing non-degradable plastic waste. However, maintaining bacterial viability and activity during culture and storage remains challenging. With the use of conventional methods, cell viability and activity was lost; therefore, these conditions need to be optimized for the practical application of microorganisms in bioplastic degradation. Therefore, we aimed to optimize the feasibility of the lyophilization method for convenient storage and direct use. In addition, we incoporated protective reagents to increase the viability and activity of lyophilized microorganisms. By selecting and applying the best protective reagents for the lyophilization process and the effects of additives on the growth and PHB-degrading activity of strains were analyzed after lyophilization. For developing the lyophilization method for protecting degradation activity, it may promote practical applications of bioplastic-degrading bacteria. Results In this study, the polyhydroxybutyrate (PHB)-degrading strain, Bacillus sp. JY14 was lyophilized with the use of various sugars as protective reagents. Among the carbon sources tested, raffinose was associated with the highest cell survival rate (12.1%). Moreover, 7% of raffionose showed the highest PHB degradation yield (92.1%). Therefore, raffinose was selected as the most effective protective reagent. Also, bacterial activity was successfully maintained, with raffinose, under different storage temperatures and period. Conclusions This study highlights lyophilization as an efficient microorganism storage method to enhance the applicability of bioplastic-degrading bacterial strains. The approach developed herein can be further studied and used to promote the application of microorganisms in bioplastic degradation. |
| first_indexed | 2024-03-09T14:48:45Z |
| format | Article |
| id | doaj.art-d4f7c5a394884171b6a05abddc5752fb |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-09T14:48:45Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-d4f7c5a394884171b6a05abddc5752fb2023-11-26T14:38:14ZengBMCMicrobial Cell Factories1475-28592023-09-0122111010.1186/s12934-023-02173-4A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagentsSu Hyun Kim0Nara Shin1Suk Jin Oh2Jeong Hyeon Hwang3Hyun Jin Kim4Shashi Kant Bhatia5Jeonghee Yun6Jae-Seok Kim7Yung-Hun Yang8Department of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Forest Products and Biotechnology, Kookmin UniversityDepartment of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of MedicineDepartment of Biological Engineering, College of Engineering, Konkuk UniversityAbstract Background Bioplastics are attracting considerable attention, owing to the increase in non-degradable waste. Using microorganisms to degrade bioplastics is a promising strategy for reducing non-degradable plastic waste. However, maintaining bacterial viability and activity during culture and storage remains challenging. With the use of conventional methods, cell viability and activity was lost; therefore, these conditions need to be optimized for the practical application of microorganisms in bioplastic degradation. Therefore, we aimed to optimize the feasibility of the lyophilization method for convenient storage and direct use. In addition, we incoporated protective reagents to increase the viability and activity of lyophilized microorganisms. By selecting and applying the best protective reagents for the lyophilization process and the effects of additives on the growth and PHB-degrading activity of strains were analyzed after lyophilization. For developing the lyophilization method for protecting degradation activity, it may promote practical applications of bioplastic-degrading bacteria. Results In this study, the polyhydroxybutyrate (PHB)-degrading strain, Bacillus sp. JY14 was lyophilized with the use of various sugars as protective reagents. Among the carbon sources tested, raffinose was associated with the highest cell survival rate (12.1%). Moreover, 7% of raffionose showed the highest PHB degradation yield (92.1%). Therefore, raffinose was selected as the most effective protective reagent. Also, bacterial activity was successfully maintained, with raffinose, under different storage temperatures and period. Conclusions This study highlights lyophilization as an efficient microorganism storage method to enhance the applicability of bioplastic-degrading bacterial strains. The approach developed herein can be further studied and used to promote the application of microorganisms in bioplastic degradation.https://doi.org/10.1186/s12934-023-02173-4BioplasticsPolyhydroxybutyrateBiodegradationLyophilizationRaffinose |
| spellingShingle | Su Hyun Kim Nara Shin Suk Jin Oh Jeong Hyeon Hwang Hyun Jin Kim Shashi Kant Bhatia Jeonghee Yun Jae-Seok Kim Yung-Hun Yang A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents Microbial Cell Factories Bioplastics Polyhydroxybutyrate Biodegradation Lyophilization Raffinose |
| title | A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents |
| title_full | A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents |
| title_fullStr | A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents |
| title_full_unstemmed | A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents |
| title_short | A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents |
| title_sort | strategy to promote the convenient storage and direct use of polyhydroxybutyrate degrading bacillus sp jy14 by lyophilization with protective reagents |
| topic | Bioplastics Polyhydroxybutyrate Biodegradation Lyophilization Raffinose |
| url | https://doi.org/10.1186/s12934-023-02173-4 |
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