Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm
Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207523000448 |
| _version_ | 1827578648447680512 |
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| author | Ergun Akturk Luís D.R. Melo Hugo Oliveira Aurélie Crabbé Tom Coenye Joana Azeredo |
| author_facet | Ergun Akturk Luís D.R. Melo Hugo Oliveira Aurélie Crabbé Tom Coenye Joana Azeredo |
| author_sort | Ergun Akturk |
| collection | DOAJ |
| description | Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents. |
| first_indexed | 2024-03-08T21:49:29Z |
| format | Article |
| id | doaj.art-d4fbe6b7984a4e5287951a9a796c6cb2 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-03-08T21:49:29Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-d4fbe6b7984a4e5287951a9a796c6cb22023-12-20T07:36:43ZengElsevierBiofilm2590-20752023-12-016100147Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilmErgun Akturk0Luís D.R. Melo1Hugo Oliveira2Aurélie Crabbé3Tom Coenye4Joana Azeredo5CEB - Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, PortugalCEB - Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, Portugal; ESCMID Study Group for Biofilms (ESGB), SwitzerlandCEB - Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, Portugal; ESCMID Study Group for Biofilms (ESGB), SwitzerlandLaboratory of Pharmaceutical Microbiology (LPM), Ghent University, Ghent, BelgiumLaboratory of Pharmaceutical Microbiology (LPM), Ghent University, Ghent, Belgium; ESCMID Study Group for Biofilms (ESGB), Switzerland; Corresponding author. Laboratory of Pharmaceutical Microbiology (LPM), Ghent University, Ghen, Belgium.CEB - Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, Portugal; ESCMID Study Group for Biofilms (ESGB), Switzerland; Corresponding author. CEB-Centre of Biological Engineering, LIBRO-Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents.http://www.sciencedirect.com/science/article/pii/S2590207523000448Pseudomonas aeruginosaStaphylococcus aureusPhage-antibiotic combinationGentamicinPhage-antibiotic synergy (PAS)Sequential treatment |
| spellingShingle | Ergun Akturk Luís D.R. Melo Hugo Oliveira Aurélie Crabbé Tom Coenye Joana Azeredo Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm Biofilm Pseudomonas aeruginosa Staphylococcus aureus Phage-antibiotic combination Gentamicin Phage-antibiotic synergy (PAS) Sequential treatment |
| title | Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| title_full | Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| title_fullStr | Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| title_full_unstemmed | Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| title_short | Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| title_sort | combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm |
| topic | Pseudomonas aeruginosa Staphylococcus aureus Phage-antibiotic combination Gentamicin Phage-antibiotic synergy (PAS) Sequential treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2590207523000448 |
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