Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>)
As a common influencing factor in the environment, temperature greatly influences the fish that live in the water all their life. The essential economic fish Chinese tongue sole (<i>Cynoglossus semilaevis</i>), a benthic fish, will experience both physiological and behavioral changes due...
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MDPI AG
2023-12-01
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| Online Access: | https://www.mdpi.com/2076-2615/14/1/84 |
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| author | Yue Wang Chengcheng Su Qian Liu Xiancai Hao Shenglei Han Lucas B. Doretto Ivana F. Rosa Yanjing Yang Changwei Shao Qian Wang |
| author_facet | Yue Wang Chengcheng Su Qian Liu Xiancai Hao Shenglei Han Lucas B. Doretto Ivana F. Rosa Yanjing Yang Changwei Shao Qian Wang |
| author_sort | Yue Wang |
| collection | DOAJ |
| description | As a common influencing factor in the environment, temperature greatly influences the fish that live in the water all their life. The essential economic fish Chinese tongue sole (<i>Cynoglossus semilaevis</i>), a benthic fish, will experience both physiological and behavioral changes due to increases in temperature. The brain, as the central hub of fish and a crucial regulatory organ, is particularly sensitive to temperature changes and will be affected. However, previous research has mainly concentrated on the impact of temperature on the gonads of <i>C. semilaevis</i>. Instead, our study examines the brain using transcriptomics to investigate specific genes and pathways that can quickly respond to temperature changes. The fish were subjected to various periods of heat stress (1 h, 2 h, 3 h, and 5 h) before extracting the brain for transcriptome analysis. After conducting transcriptomic analyses, we identified distinct genes and pathways in males and females. The pathways were mainly related to cortisol synthesis and secretion, neuroactive ligand–receptor interactions, TGF beta signaling pathway, and JAK/STAT signaling pathway, while the genes included the HSP family, <i>tshr</i>, <i>c-fos</i>, <i>c-jun</i>, <i>cxcr4</i>, <i>camk2b</i>, and <i>igf2</i>. Our study offers valuable insights into the regulation mechanisms of the brain’s response to temperature stress. |
| first_indexed | 2024-03-08T15:12:46Z |
| format | Article |
| id | doaj.art-d502003b97e945ea9e30b2c24fb7dfa8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T15:12:46Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d502003b97e945ea9e30b2c24fb7dfa82024-01-10T14:50:15ZengMDPI AGAnimals2076-26152023-12-011418410.3390/ani14010084Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>)Yue Wang0Chengcheng Su1Qian Liu2Xiancai Hao3Shenglei Han4Lucas B. Doretto5Ivana F. Rosa6Yanjing Yang7Changwei Shao8Qian Wang9Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaDepartment of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 01049-010, BrazilTianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaAs a common influencing factor in the environment, temperature greatly influences the fish that live in the water all their life. The essential economic fish Chinese tongue sole (<i>Cynoglossus semilaevis</i>), a benthic fish, will experience both physiological and behavioral changes due to increases in temperature. The brain, as the central hub of fish and a crucial regulatory organ, is particularly sensitive to temperature changes and will be affected. However, previous research has mainly concentrated on the impact of temperature on the gonads of <i>C. semilaevis</i>. Instead, our study examines the brain using transcriptomics to investigate specific genes and pathways that can quickly respond to temperature changes. The fish were subjected to various periods of heat stress (1 h, 2 h, 3 h, and 5 h) before extracting the brain for transcriptome analysis. After conducting transcriptomic analyses, we identified distinct genes and pathways in males and females. The pathways were mainly related to cortisol synthesis and secretion, neuroactive ligand–receptor interactions, TGF beta signaling pathway, and JAK/STAT signaling pathway, while the genes included the HSP family, <i>tshr</i>, <i>c-fos</i>, <i>c-jun</i>, <i>cxcr4</i>, <i>camk2b</i>, and <i>igf2</i>. Our study offers valuable insights into the regulation mechanisms of the brain’s response to temperature stress.https://www.mdpi.com/2076-2615/14/1/84<i>Cynoglossus semilaevis</i>transcriptomeheat shockgene |
| spellingShingle | Yue Wang Chengcheng Su Qian Liu Xiancai Hao Shenglei Han Lucas B. Doretto Ivana F. Rosa Yanjing Yang Changwei Shao Qian Wang Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) Animals <i>Cynoglossus semilaevis</i> transcriptome heat shock gene |
| title | Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_full | Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_fullStr | Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_full_unstemmed | Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_short | Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_sort | transcriptome analysis revealed the early heat stress response in the brain of chinese tongue sole i cynoglossus semilaevis i |
| topic | <i>Cynoglossus semilaevis</i> transcriptome heat shock gene |
| url | https://www.mdpi.com/2076-2615/14/1/84 |
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