Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico
The aim of this study was to associate <i>FGFR4</i> rs1966265 and rs351855 variants with colorectal cancer (CRC) in a Mexican population and to perform in silico analysis. Genomic DNA from 412 healthy individuals and 475 CRC patients was analyzed. In silico analysis was performed using t...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/12/3/602 |
| _version_ | 1797241963400921088 |
|---|---|
| author | Irving Alejandro Carrillo-Dávila Asbiel Felipe Garibaldi-Ríos Luis E. Figuera Belinda Claudia Gómez-Meda Guillermo M. Zúñiga-González Ana María Puebla-Pérez Patricia Montserrat García-Verdín Paola Beatriz Castro-García Itzae Adonai Gutiérrez-Hurtado Blanca Miriam Torres-Mendoza Martha Patricia Gallegos-Arreola |
| author_facet | Irving Alejandro Carrillo-Dávila Asbiel Felipe Garibaldi-Ríos Luis E. Figuera Belinda Claudia Gómez-Meda Guillermo M. Zúñiga-González Ana María Puebla-Pérez Patricia Montserrat García-Verdín Paola Beatriz Castro-García Itzae Adonai Gutiérrez-Hurtado Blanca Miriam Torres-Mendoza Martha Patricia Gallegos-Arreola |
| author_sort | Irving Alejandro Carrillo-Dávila |
| collection | DOAJ |
| description | The aim of this study was to associate <i>FGFR4</i> rs1966265 and rs351855 variants with colorectal cancer (CRC) in a Mexican population and to perform in silico analysis. Genomic DNA from 412 healthy individuals and 475 CRC patients was analyzed. In silico analysis was performed using the PolyPhen-V2, GEPIA, GTEx, and Cytoscape platforms. The GA genotype dominant model (GAAA) of rs1966265 and the AA genotype dominant and recessive models of rs351855 were identified as CRC risk factors (<i>p</i> < 0.05). CRC patients aged ≥ 50 years at diagnosis who consumed alcohol had a higher incidence of the rs351855 GA genotype than the control group (<i>p</i> < 0.05). Associations were observed between the rs1966265 GA genotype and patients with rectal cancer and stage III–IV disease. The rs351855 AA genotype was a risk factor for partial chemotherapy response, and the GA + AA genotype for age ≥ 50 years at diagnosis and rectal cancer was associated with a partial response to chemotherapy (<i>p</i> < 0.05). The AA haplotype was associated with increased susceptibility to CRC. In silico analysis indicated that the rs351855 variant is likely pathogenic (score = 0.998). Genotypic expression analysis in blood samples showed statistically significant differences (<i>p</i> < 0.05). <i>EFNA4</i>, <i>SLC3A2</i>, and <i>HNF1A</i> share signaling pathways with <i>FGFR4</i>. Therefore, rs1966265 and rs351855 may be potential CRC risk factors. |
| first_indexed | 2024-04-24T18:31:40Z |
| format | Article |
| id | doaj.art-d504c251d76f40dda8027c3a50db754b |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-04-24T18:31:40Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-d504c251d76f40dda8027c3a50db754b2024-03-27T13:22:55ZengMDPI AGBiomedicines2227-90592024-03-0112360210.3390/biomedicines12030602Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In SilicoIrving Alejandro Carrillo-Dávila0Asbiel Felipe Garibaldi-Ríos1Luis E. Figuera2Belinda Claudia Gómez-Meda3Guillermo M. Zúñiga-González4Ana María Puebla-Pérez5Patricia Montserrat García-Verdín6Paola Beatriz Castro-García7Itzae Adonai Gutiérrez-Hurtado8Blanca Miriam Torres-Mendoza9Martha Patricia Gallegos-Arreola10División de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Guadalajara 44340, Jalisco, MexicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoLaboratorio de Inmunofarmacología, Centro Universitario de Ciencias Exactas e Ingenierías (CUCEI), Universidad de Guadalajara (UdeG), Guadalajara 44430, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoLaboratorio de Inmunofarmacología, Centro Universitario de Ciencias Exactas e Ingenierías (CUCEI), Universidad de Guadalajara (UdeG), Guadalajara 44430, Jalisco, MexicoDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Guadalajara 44340, Jalisco, MexicoLaboratorio de Inmunodeficiencias Humanas y Retrovirus, División de Neurociencias, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoThe aim of this study was to associate <i>FGFR4</i> rs1966265 and rs351855 variants with colorectal cancer (CRC) in a Mexican population and to perform in silico analysis. Genomic DNA from 412 healthy individuals and 475 CRC patients was analyzed. In silico analysis was performed using the PolyPhen-V2, GEPIA, GTEx, and Cytoscape platforms. The GA genotype dominant model (GAAA) of rs1966265 and the AA genotype dominant and recessive models of rs351855 were identified as CRC risk factors (<i>p</i> < 0.05). CRC patients aged ≥ 50 years at diagnosis who consumed alcohol had a higher incidence of the rs351855 GA genotype than the control group (<i>p</i> < 0.05). Associations were observed between the rs1966265 GA genotype and patients with rectal cancer and stage III–IV disease. The rs351855 AA genotype was a risk factor for partial chemotherapy response, and the GA + AA genotype for age ≥ 50 years at diagnosis and rectal cancer was associated with a partial response to chemotherapy (<i>p</i> < 0.05). The AA haplotype was associated with increased susceptibility to CRC. In silico analysis indicated that the rs351855 variant is likely pathogenic (score = 0.998). Genotypic expression analysis in blood samples showed statistically significant differences (<i>p</i> < 0.05). <i>EFNA4</i>, <i>SLC3A2</i>, and <i>HNF1A</i> share signaling pathways with <i>FGFR4</i>. Therefore, rs1966265 and rs351855 may be potential CRC risk factors.https://www.mdpi.com/2227-9059/12/3/602colorectal neoplasms<i>FGFR4</i> genevariantsingle nucleotidein silico analysisgenetic variation |
| spellingShingle | Irving Alejandro Carrillo-Dávila Asbiel Felipe Garibaldi-Ríos Luis E. Figuera Belinda Claudia Gómez-Meda Guillermo M. Zúñiga-González Ana María Puebla-Pérez Patricia Montserrat García-Verdín Paola Beatriz Castro-García Itzae Adonai Gutiérrez-Hurtado Blanca Miriam Torres-Mendoza Martha Patricia Gallegos-Arreola Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico Biomedicines colorectal neoplasms <i>FGFR4</i> gene variant single nucleotide in silico analysis genetic variation |
| title | Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_full | Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_fullStr | Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_full_unstemmed | Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_short | Association of the rs1966265 and rs351855 <i>FGFR4</i> Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_sort | association of the rs1966265 and rs351855 i fgfr4 i variants with colorectal cancer in a mexican population and their analysis in silico |
| topic | colorectal neoplasms <i>FGFR4</i> gene variant single nucleotide in silico analysis genetic variation |
| url | https://www.mdpi.com/2227-9059/12/3/602 |
| work_keys_str_mv | AT irvingalejandrocarrillodavila associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT asbielfelipegaribaldirios associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT luisefiguera associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT belindaclaudiagomezmeda associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT guillermomzunigagonzalez associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT anamariapueblaperez associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT patriciamontserratgarciaverdin associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT paolabeatrizcastrogarcia associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT itzaeadonaigutierrezhurtado associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT blancamiriamtorresmendoza associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico AT marthapatriciagallegosarreola associationofthers1966265andrs351855ifgfr4ivariantswithcolorectalcancerinamexicanpopulationandtheiranalysisinsilico |