Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells
Natural polyphenol-rich plant resources, such as agricultural waste, were proven to diminish insulin resistance and weight gain in rats on a high-fat diet. To test whether date seed polyphenol pills (DSPPs) might lower adipose tissue accumulation by precisely affecting adipocytes, we explored the im...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/22/12533 |
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| author | Remah Sobhy Xiaobo Zou Osama M. Morsy Ahmed A. Zaky Ibrahim Khalifa |
| author_facet | Remah Sobhy Xiaobo Zou Osama M. Morsy Ahmed A. Zaky Ibrahim Khalifa |
| author_sort | Remah Sobhy |
| collection | DOAJ |
| description | Natural polyphenol-rich plant resources, such as agricultural waste, were proven to diminish insulin resistance and weight gain in rats on a high-fat diet. To test whether date seed polyphenol pills (DSPPs) might lower adipose tissue accumulation by precisely affecting adipocytes, we explored the impacts of DSPPs on cell proliferation, differentiation, and lipolysis in 3T3-L1 cells. We utilized tablets made commercially from date seed polyphenols that were mostly composed of epicatechin (45.9 g/kg). The total polyphenol and antioxidant capacities of the digested and non-digested DSPPs were also evaluated. DSPPs at doses of 25, 50, and 100 µg/mL hindered the proliferation of both pre-confluent preadipocytes and mature post-confluent adipocytes. DSPPs decreased the quantity of viable cells in completely developed adipocytes. Treatment with 100 µg/mL of DSPPs decreased the basal lipolysis of completely differentiated adipocytes but modestly boosted epinephrine-induced lipolysis. A significant transcription factor for the adipogenic gene, the peroxisome proliferator-activated receptor (PPAR), was repressed by DSPPs, which significantly decreased lipid buildup. The total polyphenol and antioxidant capacities were also increased after digestion with a good bubble Pearson correlation between both. DSPPs may have anti-obesity and anti-diabetic characteristics by inhibiting adipocyte development and basal lipolysis, which could be commercially industrialized. |
| first_indexed | 2024-03-09T17:02:55Z |
| format | Article |
| id | doaj.art-d505a9f44014451cb085fee437f02402 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:02:55Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d505a9f44014451cb085fee437f024022023-11-24T14:28:20ZengMDPI AGApplied Sciences2076-34172023-11-0113221253310.3390/app132212533Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 CellsRemah Sobhy0Xiaobo Zou1Osama M. Morsy2Ahmed A. Zaky3Ibrahim Khalifa4Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaAgricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaFaculty of Engineering, Department of Basic and Applied Sciences, Arab Academy of Science, Technology and Maritime Transport, Cairo P.O. Box 2033, EgyptDepartment of Food Technology, Food Industries and Nutrition Research Institute, National Research Centre, Dokki, Cairo 12622, EgyptAgricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaNatural polyphenol-rich plant resources, such as agricultural waste, were proven to diminish insulin resistance and weight gain in rats on a high-fat diet. To test whether date seed polyphenol pills (DSPPs) might lower adipose tissue accumulation by precisely affecting adipocytes, we explored the impacts of DSPPs on cell proliferation, differentiation, and lipolysis in 3T3-L1 cells. We utilized tablets made commercially from date seed polyphenols that were mostly composed of epicatechin (45.9 g/kg). The total polyphenol and antioxidant capacities of the digested and non-digested DSPPs were also evaluated. DSPPs at doses of 25, 50, and 100 µg/mL hindered the proliferation of both pre-confluent preadipocytes and mature post-confluent adipocytes. DSPPs decreased the quantity of viable cells in completely developed adipocytes. Treatment with 100 µg/mL of DSPPs decreased the basal lipolysis of completely differentiated adipocytes but modestly boosted epinephrine-induced lipolysis. A significant transcription factor for the adipogenic gene, the peroxisome proliferator-activated receptor (PPAR), was repressed by DSPPs, which significantly decreased lipid buildup. The total polyphenol and antioxidant capacities were also increased after digestion with a good bubble Pearson correlation between both. DSPPs may have anti-obesity and anti-diabetic characteristics by inhibiting adipocyte development and basal lipolysis, which could be commercially industrialized.https://www.mdpi.com/2076-3417/13/22/12533agricultural wastedate seed polyphenolsindustrial-scale pills3T3-L1 cellsobesity |
| spellingShingle | Remah Sobhy Xiaobo Zou Osama M. Morsy Ahmed A. Zaky Ibrahim Khalifa Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells Applied Sciences agricultural waste date seed polyphenols industrial-scale pills 3T3-L1 cells obesity |
| title | Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells |
| title_full | Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells |
| title_fullStr | Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells |
| title_full_unstemmed | Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells |
| title_short | Date Seed Polyphenol Pills as Renewable Raw Materials Showed Anti-Obesity Effects with High Digestible Antioxidants in 3T3-L1 Cells |
| title_sort | date seed polyphenol pills as renewable raw materials showed anti obesity effects with high digestible antioxidants in 3t3 l1 cells |
| topic | agricultural waste date seed polyphenols industrial-scale pills 3T3-L1 cells obesity |
| url | https://www.mdpi.com/2076-3417/13/22/12533 |
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