Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function
The tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2018-11-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200608&lng=en&tlng=en |
| _version_ | 1811219110702350336 |
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| author | Enxu Bi Dengqiang Liu Youxi Li Xuying Mao Aihua Wang Jingtao Wang |
| author_facet | Enxu Bi Dengqiang Liu Youxi Li Xuying Mao Aihua Wang Jingtao Wang |
| author_sort | Enxu Bi |
| collection | DOAJ |
| description | The tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell numbers, cell migration, survival fraction, and cell viability were, respectively, evaluated by trypan blue exclusion assay, wound healing assay, clonogenic assay, and CCK-8 assay. Cell apoptosis was determined by flow cytometry assay and western blot. The expression of p53 was inhibited by transient transfection, and the efficiency was verified by western blot. qRT-PCR was performed to measure the mRNA expression of p53. Western blot was used to evaluate the protein expression of apoptosis, DNA damage and p53 function related factors. We found that oridonin significantly inhibited cell proliferation, migration, and survivability, and enhanced cell apoptosis in SNU-216 cells. However, it had no influence on HEK293 cell viability. Oridonin also remarkably enhanced the anti-tumor effect of cisplatin on SNU-216 cells, as it significantly increased apoptotic cells and decreased cell viability. Moreover, the mRNA and protein expression of p53 was significantly up-regulated in oridonin-treated cells, while Mdm2 expression was down-regulated. Furthermore, oridonin enhanced p53 function and induced DNA damage. Knockdown of p53 or employing the caspase inhibitor, Boc-D-FMK, reversed the effect of oridonin on cell viability and apoptosis-related protein expression. The present study demonstrated that oridonin exhibited an anti-tumor effect on GC SNU-216 cells through regulating p53 expression and function. |
| first_indexed | 2024-04-12T07:19:47Z |
| format | Article |
| id | doaj.art-d5076e79a806481cb70fe69962b43adb |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-12T07:19:47Z |
| publishDate | 2018-11-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-d5076e79a806481cb70fe69962b43adb2022-12-22T03:42:21ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2018-11-01511210.1590/1414-431x20187599S0100-879X2018001200608Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and functionEnxu BiDengqiang LiuYouxi LiXuying MaoAihua WangJingtao WangThe tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell numbers, cell migration, survival fraction, and cell viability were, respectively, evaluated by trypan blue exclusion assay, wound healing assay, clonogenic assay, and CCK-8 assay. Cell apoptosis was determined by flow cytometry assay and western blot. The expression of p53 was inhibited by transient transfection, and the efficiency was verified by western blot. qRT-PCR was performed to measure the mRNA expression of p53. Western blot was used to evaluate the protein expression of apoptosis, DNA damage and p53 function related factors. We found that oridonin significantly inhibited cell proliferation, migration, and survivability, and enhanced cell apoptosis in SNU-216 cells. However, it had no influence on HEK293 cell viability. Oridonin also remarkably enhanced the anti-tumor effect of cisplatin on SNU-216 cells, as it significantly increased apoptotic cells and decreased cell viability. Moreover, the mRNA and protein expression of p53 was significantly up-regulated in oridonin-treated cells, while Mdm2 expression was down-regulated. Furthermore, oridonin enhanced p53 function and induced DNA damage. Knockdown of p53 or employing the caspase inhibitor, Boc-D-FMK, reversed the effect of oridonin on cell viability and apoptosis-related protein expression. The present study demonstrated that oridonin exhibited an anti-tumor effect on GC SNU-216 cells through regulating p53 expression and function.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200608&lng=en&tlng=enOridoninp53Gastric cancerCell apoptosisMdm2 |
| spellingShingle | Enxu Bi Dengqiang Liu Youxi Li Xuying Mao Aihua Wang Jingtao Wang Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function Brazilian Journal of Medical and Biological Research Oridonin p53 Gastric cancer Cell apoptosis Mdm2 |
| title | Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| title_full | Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| title_fullStr | Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| title_full_unstemmed | Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| title_short | Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| title_sort | oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function |
| topic | Oridonin p53 Gastric cancer Cell apoptosis Mdm2 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200608&lng=en&tlng=en |
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