Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran
Background: Geothermal waters could be suitable niches for thermophilic free living amoebae including Naegleria and Hartmannella. Ardebil Province, northwest Iran is popular for having many hot springs for recreational and health purposes activity. The present research is the first molecular based i...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-06-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20958.pdf&manuscript_id=20958 |
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| author | E Nazemalhosseini Mojarad A Haghighi M Niyyati R Solgi |
| author_facet | E Nazemalhosseini Mojarad A Haghighi M Niyyati R Solgi |
| author_sort | E Nazemalhosseini Mojarad |
| collection | DOAJ |
| description | Background: Geothermal waters could be suitable niches for thermophilic free living amoebae including Naegleria and Hartmannella. Ardebil Province, northwest Iran is popular for having many hot springs for recreational and health purposes activity. The present research is the first molecular based investigation regarding the presence of Naegleria and Hartmannella in the hot springs of Ardebil Province in Iran.Methods: Overall, 30 water samples were taken from waters of thermal hot springs in Ardebil Province, Iran during 2010-2011. All collected samples were transferred to Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Cultivation of concentrated water samples was performed using culture-enrichment method. Cloning of the target amoebae was obtained and morphological and molecular analysis was done using page key combined with two sets of primers, respectively. Sequence analysis and homology search was used for strains identification.Results: Of 30 water samples, 8 (26.7%) were positive for thermotolerant Vahlkampfiids and Hartman­nella based on morphological characteristics of vegetative form and double walled cysts. Cloning of the target amoebae were done successfully. Sequencing of the positive isolates revealed that the strains belonged to Naegleria (N. carteri and N. spp) and H. vermiformis.Conclusion: The result highlights a need for improved filtration and disinfection and periodic monitoring of recreational thermal waters in order to prevent disease related to free- living amoebae. This is the first comprehensive molecular study of thermophilic Naegleria and Hartmannella in hot springs of Iran. |
| first_indexed | 2024-12-17T07:48:34Z |
| format | Article |
| id | doaj.art-d508295af31b409ca456fbf4a69aca24 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-17T07:48:34Z |
| publishDate | 2012-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-d508295af31b409ca456fbf4a69aca242022-12-21T21:57:55ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2012-06-01724752Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest IranE Nazemalhosseini MojaradA HaghighiM NiyyatiR SolgiBackground: Geothermal waters could be suitable niches for thermophilic free living amoebae including Naegleria and Hartmannella. Ardebil Province, northwest Iran is popular for having many hot springs for recreational and health purposes activity. The present research is the first molecular based investigation regarding the presence of Naegleria and Hartmannella in the hot springs of Ardebil Province in Iran.Methods: Overall, 30 water samples were taken from waters of thermal hot springs in Ardebil Province, Iran during 2010-2011. All collected samples were transferred to Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Cultivation of concentrated water samples was performed using culture-enrichment method. Cloning of the target amoebae was obtained and morphological and molecular analysis was done using page key combined with two sets of primers, respectively. Sequence analysis and homology search was used for strains identification.Results: Of 30 water samples, 8 (26.7%) were positive for thermotolerant Vahlkampfiids and Hartman­nella based on morphological characteristics of vegetative form and double walled cysts. Cloning of the target amoebae were done successfully. Sequencing of the positive isolates revealed that the strains belonged to Naegleria (N. carteri and N. spp) and H. vermiformis.Conclusion: The result highlights a need for improved filtration and disinfection and periodic monitoring of recreational thermal waters in order to prevent disease related to free- living amoebae. This is the first comprehensive molecular study of thermophilic Naegleria and Hartmannella in hot springs of Iran.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20958.pdf&manuscript_id=20958Hot SpringsHartmannellaNaegleriaIran |
| spellingShingle | E Nazemalhosseini Mojarad A Haghighi M Niyyati R Solgi Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran Iranian Journal of Parasitology Hot Springs Hartmannella Naegleria Iran |
| title | Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran |
| title_full | Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran |
| title_fullStr | Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran |
| title_full_unstemmed | Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran |
| title_short | Occurrence of Thermotolerant Hartmannella Vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran |
| title_sort | occurrence of thermotolerant hartmannella vermiformis and naegleria spp in hot springs of ardebil province northwest iran |
| topic | Hot Springs Hartmannella Naegleria Iran |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20958.pdf&manuscript_id=20958 |
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