Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators
Bioactive molecules of plant origin play a significant role as defensive agents in different insect species. Chemical compounds in medicinal plants have been an exciting alternative to standard methods of controlling mosquito larvae. The present study evaluates the different solvent extracts of <...
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MDPI AG
2021-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/7/1267 |
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| author | Palanisamy Prakash Ekambaram Gayathiri Rengarajan Manivasagaperumal Patcharin Krutmuang |
| author_facet | Palanisamy Prakash Ekambaram Gayathiri Rengarajan Manivasagaperumal Patcharin Krutmuang |
| author_sort | Palanisamy Prakash |
| collection | DOAJ |
| description | Bioactive molecules of plant origin play a significant role as defensive agents in different insect species. Chemical compounds in medicinal plants have been an exciting alternative to standard methods of controlling mosquito larvae. The present study evaluates the different solvent extracts of <i>D. hamiltonii</i> for toxicity against three different mosquito larvae. Bioassay revealed that the effect of the methanol extracts increased the larval mortality with increasing concentration. The highest larval mortality was observed in Culex quinquefasciatus with 98.33%, followed by 95 and 90% mortality in Aedes aegypti and Anopheles stephensi, at 24 h exposure. GC-MS analysis of methanol extract of <i>D. hamiltonii</i> showed six major peak compounds. They are benzaldehyde, 2-hydroxy-4-methoxy-(10.35%), dodecanoic acid (11.02%), n-hexadecanoic acid (21.05%), linoleic acid methyl ester (14.20%), oleic acid (21.04%), octadecanoic acid (22.21%). The level of α and β Carboxylesterases gets significantly decreased post-treatment with the methanol extract of <i>D. hamiltonii</i> in a dose-dependent manner.In contrast, glutathione S-transferase (GST) and cytochrome-P450 (CYP450) levels get up-lifted steadily when the dosage gets increased. The ratio of GST level has drastically proclaimed to in <i>Ae. aegypti</i> 0.702 mg/m Lin parallel to <i>Cx. quiquefasciatus</i> (0.656 mg/mL) and <i>An. stephensi</i> (0.812 mg/mL). Cytochrome P450 (CYP450) activity was observed to increase significantly post-treatment with the sub-lethal dosage of methanol extract of <i>D. hamiltonii</i>. Correspondingly, the non-target screening against the aquatic predators reveals that the crude root extracts and their derivatives are ecologically safe and less toxic. Overall, the present research highlights the chemical characterization of crude methanol extracts of <i>D. hamiltonii</i>, their insecticidal activity against the medically challenging pests, and their non-target activity delivers an ecologically safe, and target specific bio-active agents and suitable substitute for chemical pesticides. |
| first_indexed | 2024-03-10T10:11:06Z |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T10:11:06Z |
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| spelling | doaj.art-d509893016be4cc6aa942704ee42fb412023-11-22T01:13:16ZengMDPI AGAgronomy2073-43952021-06-01117126710.3390/agronomy11071267Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito PredatorsPalanisamy Prakash0Ekambaram Gayathiri1Rengarajan Manivasagaperumal2Patcharin Krutmuang3Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, IndiaDepartment of Plant Biology and Plant Biotechnology, Guru Nanak College (Autonomous), Chennai 600042, IndiaDepartment of Botany, Thiru Kolanjiyapper Government Arts College—Virudhachalam, Cuddalore 607402, Tamil Nadu, IndiaDepartment of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandBioactive molecules of plant origin play a significant role as defensive agents in different insect species. Chemical compounds in medicinal plants have been an exciting alternative to standard methods of controlling mosquito larvae. The present study evaluates the different solvent extracts of <i>D. hamiltonii</i> for toxicity against three different mosquito larvae. Bioassay revealed that the effect of the methanol extracts increased the larval mortality with increasing concentration. The highest larval mortality was observed in Culex quinquefasciatus with 98.33%, followed by 95 and 90% mortality in Aedes aegypti and Anopheles stephensi, at 24 h exposure. GC-MS analysis of methanol extract of <i>D. hamiltonii</i> showed six major peak compounds. They are benzaldehyde, 2-hydroxy-4-methoxy-(10.35%), dodecanoic acid (11.02%), n-hexadecanoic acid (21.05%), linoleic acid methyl ester (14.20%), oleic acid (21.04%), octadecanoic acid (22.21%). The level of α and β Carboxylesterases gets significantly decreased post-treatment with the methanol extract of <i>D. hamiltonii</i> in a dose-dependent manner.In contrast, glutathione S-transferase (GST) and cytochrome-P450 (CYP450) levels get up-lifted steadily when the dosage gets increased. The ratio of GST level has drastically proclaimed to in <i>Ae. aegypti</i> 0.702 mg/m Lin parallel to <i>Cx. quiquefasciatus</i> (0.656 mg/mL) and <i>An. stephensi</i> (0.812 mg/mL). Cytochrome P450 (CYP450) activity was observed to increase significantly post-treatment with the sub-lethal dosage of methanol extract of <i>D. hamiltonii</i>. Correspondingly, the non-target screening against the aquatic predators reveals that the crude root extracts and their derivatives are ecologically safe and less toxic. Overall, the present research highlights the chemical characterization of crude methanol extracts of <i>D. hamiltonii</i>, their insecticidal activity against the medically challenging pests, and their non-target activity delivers an ecologically safe, and target specific bio-active agents and suitable substitute for chemical pesticides.https://www.mdpi.com/2073-4395/11/7/1267<i>D. hamiltonii</i>methanolCarboxylesterasesCYP450mosquito predators |
| spellingShingle | Palanisamy Prakash Ekambaram Gayathiri Rengarajan Manivasagaperumal Patcharin Krutmuang Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators Agronomy <i>D. hamiltonii</i> methanol Carboxylesterases CYP450 mosquito predators |
| title | Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators |
| title_full | Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators |
| title_fullStr | Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators |
| title_full_unstemmed | Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators |
| title_short | Biological Activity of Root Extract <i>Decalepis hamiltonii</i> (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators |
| title_sort | biological activity of root extract i decalepis hamiltonii i wight arn against three mosquito vectors and their non toxicity against the mosquito predators |
| topic | <i>D. hamiltonii</i> methanol Carboxylesterases CYP450 mosquito predators |
| url | https://www.mdpi.com/2073-4395/11/7/1267 |
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