ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma
Abstract Background Glioma is the most common and aggressive tumor in the adult brain. Recent studies have indicated that Zinc finger DHHC-type palmitoyltransferases (ZDHHCs) play vital roles in regulating the progression of glioma. ZDHHC15, a member of the ZDHHCs family, participates in various phy...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-10883-6 |
| _version_ | 1797827536713940992 |
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| author | Zhen-Yuan Liu Tian Lan Feng Tang Yong-Ze He Jin-Sheng Liu Jin-Zhou Yang Xi Chen Ze-Fen Wang Zhi-Qiang Li |
| author_facet | Zhen-Yuan Liu Tian Lan Feng Tang Yong-Ze He Jin-Sheng Liu Jin-Zhou Yang Xi Chen Ze-Fen Wang Zhi-Qiang Li |
| author_sort | Zhen-Yuan Liu |
| collection | DOAJ |
| description | Abstract Background Glioma is the most common and aggressive tumor in the adult brain. Recent studies have indicated that Zinc finger DHHC-type palmitoyltransferases (ZDHHCs) play vital roles in regulating the progression of glioma. ZDHHC15, a member of the ZDHHCs family, participates in various physiological activities in the brain. However, the biological functions and related mechanisms of ZDHHC15 in glioma remain poorly understood. Methods Data from multiple glioma-associated datasets were used to investigate the expression profiles and potential biological functions of ZDHHC15 in glioma. Expression of ZDHHC15 and its association with clinicopathological characteristics in glioma were validated by quantitative reverse transcription PCR (RT-qPCR) and immunohistochemical experiments. GO enrichment analysis, KEGG analysis, GSEA analysis, CCK-8, EdU, transwell, and western blotting assays were performed to confirm the functions and mechanism of ZDHHC15 in glioma. Moreover, we performed Kaplan-Meier analysis and Cox progression analysis to explore the prognostic significance of ZDHHC15 in glioma patients. Results ZDHHC15 expression was significantly up-regulated in glioma and positively associated with malignant phenotypes. Results from the GO and KEGG enrichment analysis revealed that ZDHHC15 was involved in regulating cell cycle and migration. Knockdown of ZDHHC15 inhibited glioma cell proliferation and migration, while overexpression of ZDHHC15 presented opposite effects on glioma cells. Besides, results from GSEA analysis suggested that ZDHHC15 was enriched in STAT3 signaling pathway. Knockdown or overexpression of ZDHHC15 indeed affected the activation of STAT3 signaling pathway. Additionally, we identified ZDHHC15 as an independent prognostic biomarker in glioma, and higher expression of ZDHHC15 predicted a poorer prognosis in glioma patients. Conclusion Our findings suggest that ZDHHC15 promotes glioma malignancy and can serve as a novel prognostic biomarker for glioma patients. Targeting ZDHHC15 may be a promising therapeutic strategy for glioma. |
| first_indexed | 2024-04-09T12:49:54Z |
| format | Article |
| id | doaj.art-d50a8e13ef0140bc8d7431196fc787c7 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-09T12:49:54Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-d50a8e13ef0140bc8d7431196fc787c72023-05-14T11:19:46ZengBMCBMC Cancer1471-24072023-05-0123111410.1186/s12885-023-10883-6ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with gliomaZhen-Yuan Liu0Tian Lan1Feng Tang2Yong-Ze He3Jin-Sheng Liu4Jin-Zhou Yang5Xi Chen6Ze-Fen Wang7Zhi-Qiang Li8Brain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityDepartment of Physiology, Wuhan University School of Basic Medical SciencesBrain Glioma Center, Department of Neurosurgery, Zhongnan Hospital of Wuhan UniversityAbstract Background Glioma is the most common and aggressive tumor in the adult brain. Recent studies have indicated that Zinc finger DHHC-type palmitoyltransferases (ZDHHCs) play vital roles in regulating the progression of glioma. ZDHHC15, a member of the ZDHHCs family, participates in various physiological activities in the brain. However, the biological functions and related mechanisms of ZDHHC15 in glioma remain poorly understood. Methods Data from multiple glioma-associated datasets were used to investigate the expression profiles and potential biological functions of ZDHHC15 in glioma. Expression of ZDHHC15 and its association with clinicopathological characteristics in glioma were validated by quantitative reverse transcription PCR (RT-qPCR) and immunohistochemical experiments. GO enrichment analysis, KEGG analysis, GSEA analysis, CCK-8, EdU, transwell, and western blotting assays were performed to confirm the functions and mechanism of ZDHHC15 in glioma. Moreover, we performed Kaplan-Meier analysis and Cox progression analysis to explore the prognostic significance of ZDHHC15 in glioma patients. Results ZDHHC15 expression was significantly up-regulated in glioma and positively associated with malignant phenotypes. Results from the GO and KEGG enrichment analysis revealed that ZDHHC15 was involved in regulating cell cycle and migration. Knockdown of ZDHHC15 inhibited glioma cell proliferation and migration, while overexpression of ZDHHC15 presented opposite effects on glioma cells. Besides, results from GSEA analysis suggested that ZDHHC15 was enriched in STAT3 signaling pathway. Knockdown or overexpression of ZDHHC15 indeed affected the activation of STAT3 signaling pathway. Additionally, we identified ZDHHC15 as an independent prognostic biomarker in glioma, and higher expression of ZDHHC15 predicted a poorer prognosis in glioma patients. Conclusion Our findings suggest that ZDHHC15 promotes glioma malignancy and can serve as a novel prognostic biomarker for glioma patients. Targeting ZDHHC15 may be a promising therapeutic strategy for glioma.https://doi.org/10.1186/s12885-023-10883-6Zinc finger DHHC-type palmitoyltransferase 15PrognosisProliferationMigrationSTAT3 signaling pathwayGlioma |
| spellingShingle | Zhen-Yuan Liu Tian Lan Feng Tang Yong-Ze He Jin-Sheng Liu Jin-Zhou Yang Xi Chen Ze-Fen Wang Zhi-Qiang Li ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma BMC Cancer Zinc finger DHHC-type palmitoyltransferase 15 Prognosis Proliferation Migration STAT3 signaling pathway Glioma |
| title | ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| title_full | ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| title_fullStr | ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| title_full_unstemmed | ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| title_short | ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| title_sort | zdhhc15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma |
| topic | Zinc finger DHHC-type palmitoyltransferase 15 Prognosis Proliferation Migration STAT3 signaling pathway Glioma |
| url | https://doi.org/10.1186/s12885-023-10883-6 |
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