Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression
In this study, we showed that PI3K/Akt signaling mediates fucoidan’s anticancer effects on prostate cancer cells, including suppression of proliferation. Fucoidan significantly decreased viability of DU-145 cancer cells in a concentration-dependent manner as shown by MTT [3-(4,5-dimethylthiazol-2-yl...
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MDPI AG
2016-07-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/14/7/126 |
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| author | Gang-Sik Choo Hae-Nim Lee Seong-Ah Shin Hyeong-Jin Kim Ji-Youn Jung |
| author_facet | Gang-Sik Choo Hae-Nim Lee Seong-Ah Shin Hyeong-Jin Kim Ji-Youn Jung |
| author_sort | Gang-Sik Choo |
| collection | DOAJ |
| description | In this study, we showed that PI3K/Akt signaling mediates fucoidan’s anticancer effects on prostate cancer cells, including suppression of proliferation. Fucoidan significantly decreased viability of DU-145 cancer cells in a concentration-dependent manner as shown by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The drug also significantly increased chromatin condensation, which indicates apoptosis, in a concentration-dependent manner as shown by DAPI (4′,6-diamidino-2-phenylindole) staining. Fucoidan increased expression of Bax, cleaved poly-ADP ribose polymerase and cleaved caspase-9, and decreased of the Bcl-2, p-Akt, p-PI3K, p-P38, and p-ERK in a concentration-dependent manner. In vivo, fucoidan (at 5 and 10 mg/kg) significantly decreased tumor volume, and increased apoptosis as assessed by the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay, confirming the tumor inhibitory effect. The drug also increased expression of p-Akt and p-ERK as shown by immunohistochemistry staining. Therefore, fucoidan may be a promising cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human prostate cancer cells. |
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| id | doaj.art-d50ba1b8e0e64ce6a246d819c2b85301 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:26:01Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-d50ba1b8e0e64ce6a246d819c2b853012022-12-22T03:59:43ZengMDPI AGMarine Drugs1660-33972016-07-0114712610.3390/md14070126md14070126Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway ExpressionGang-Sik Choo0Hae-Nim Lee1Seong-Ah Shin2Hyeong-Jin Kim3Ji-Youn Jung4Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, KoreaIn this study, we showed that PI3K/Akt signaling mediates fucoidan’s anticancer effects on prostate cancer cells, including suppression of proliferation. Fucoidan significantly decreased viability of DU-145 cancer cells in a concentration-dependent manner as shown by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The drug also significantly increased chromatin condensation, which indicates apoptosis, in a concentration-dependent manner as shown by DAPI (4′,6-diamidino-2-phenylindole) staining. Fucoidan increased expression of Bax, cleaved poly-ADP ribose polymerase and cleaved caspase-9, and decreased of the Bcl-2, p-Akt, p-PI3K, p-P38, and p-ERK in a concentration-dependent manner. In vivo, fucoidan (at 5 and 10 mg/kg) significantly decreased tumor volume, and increased apoptosis as assessed by the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay, confirming the tumor inhibitory effect. The drug also increased expression of p-Akt and p-ERK as shown by immunohistochemistry staining. Therefore, fucoidan may be a promising cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human prostate cancer cells.http://www.mdpi.com/1660-3397/14/7/126apoptosisfucoidanhuman prostate cancerphosphoinositide 3-kinaseAktmitogen-activated protein kinases |
| spellingShingle | Gang-Sik Choo Hae-Nim Lee Seong-Ah Shin Hyeong-Jin Kim Ji-Youn Jung Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression Marine Drugs apoptosis fucoidan human prostate cancer phosphoinositide 3-kinase Akt mitogen-activated protein kinases |
| title | Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression |
| title_full | Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression |
| title_fullStr | Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression |
| title_full_unstemmed | Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression |
| title_short | Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression |
| title_sort | anticancer effect of fucoidan on du 145 prostate cancer cells through inhibition of pi3k akt and mapk pathway expression |
| topic | apoptosis fucoidan human prostate cancer phosphoinositide 3-kinase Akt mitogen-activated protein kinases |
| url | http://www.mdpi.com/1660-3397/14/7/126 |
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