Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran
Isolates were identified by molecular and morphological tests, including coleopteran-specific cry genes in the Iranian native Bacillus thuringiensis collection. Spherical and irregular shapes were observed to be the most frequent shapes using Coomassie brilliant blue staining. PCR analysis with univ...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2022-01-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-59810-en.html |
| _version_ | 1828068361027715072 |
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| author | Pariya Soltani-Nezhad Fariba Mehrkhou Maryam Rashki |
| author_facet | Pariya Soltani-Nezhad Fariba Mehrkhou Maryam Rashki |
| author_sort | Pariya Soltani-Nezhad |
| collection | DOAJ |
| description | Isolates were identified by molecular and morphological tests, including coleopteran-specific cry genes in the Iranian native Bacillus thuringiensis collection. Spherical and irregular shapes were observed to be the most frequent shapes using Coomassie brilliant blue staining. PCR analysis with universal and specific primer pairs was used to detect coleopteran-specific cry genes such as cry1I, cry3, cry7, cry18, and cry26. All the isolates contained at least one active coleopteran-cry gene, while the most abundant isolates had cry26 and cry18 genes. The patterns of protein size were characterized in addition to their insecticidal activity against third-instar larvae of Tribolium castaneum. Protein profiles produced bands that varied from 14-180 kDa. Four native isolates containing coleopteran-active cry genes displayed higher activity against T. castaneum larvae than B. thuringiensis subspecies galleriae as a reference strain. The median lethal concentration (LC50) of the most pathogenic isolate, PS1078, was 2.72 × 106 spores/ml. Its 16S rDNA gene sequence analysis demonstrated similarity to B. thuringiensiss subspecies galleriae. The characterization of isolates provided useful data for selecting new isolates to expand novel bio-insecticidal products. |
| first_indexed | 2024-04-11T00:00:29Z |
| format | Article |
| id | doaj.art-d50ba75f43a44926a7559470df7e7740 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-11T00:00:29Z |
| publishDate | 2022-01-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-d50ba75f43a44926a7559470df7e77402023-01-10T06:00:57ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2022-01-01114457470Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of IranPariya Soltani-Nezhad0Fariba Mehrkhou1Maryam Rashki2 Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Isolates were identified by molecular and morphological tests, including coleopteran-specific cry genes in the Iranian native Bacillus thuringiensis collection. Spherical and irregular shapes were observed to be the most frequent shapes using Coomassie brilliant blue staining. PCR analysis with universal and specific primer pairs was used to detect coleopteran-specific cry genes such as cry1I, cry3, cry7, cry18, and cry26. All the isolates contained at least one active coleopteran-cry gene, while the most abundant isolates had cry26 and cry18 genes. The patterns of protein size were characterized in addition to their insecticidal activity against third-instar larvae of Tribolium castaneum. Protein profiles produced bands that varied from 14-180 kDa. Four native isolates containing coleopteran-active cry genes displayed higher activity against T. castaneum larvae than B. thuringiensis subspecies galleriae as a reference strain. The median lethal concentration (LC50) of the most pathogenic isolate, PS1078, was 2.72 × 106 spores/ml. Its 16S rDNA gene sequence analysis demonstrated similarity to B. thuringiensiss subspecies galleriae. The characterization of isolates provided useful data for selecting new isolates to expand novel bio-insecticidal products.http://jcp.modares.ac.ir/article-3-59810-en.htmlbacillus thuringiensiscoleopteran-specific cry genestribolium castaneumplasmidprotein profiles |
| spellingShingle | Pariya Soltani-Nezhad Fariba Mehrkhou Maryam Rashki Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran Journal of Crop Protection bacillus thuringiensis coleopteran-specific cry genes tribolium castaneum plasmid protein profiles |
| title | Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran |
| title_full | Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran |
| title_fullStr | Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran |
| title_full_unstemmed | Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran |
| title_short | Diversity of coleopteran-specific cry genes of Bacillus thuringiensis strains isolated from soil of some east and south regions of Iran |
| title_sort | diversity of coleopteran specific cry genes of bacillus thuringiensis strains isolated from soil of some east and south regions of iran |
| topic | bacillus thuringiensis coleopteran-specific cry genes tribolium castaneum plasmid protein profiles |
| url | http://jcp.modares.ac.ir/article-3-59810-en.html |
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