MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function class...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=11;spage=1799;epage=1808;aulast=Min |
| _version_ | 1818848096328613888 |
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| author | Xiao-li Min Ting-yong Wang Yi Cao Jia Liu Jin-tao Li Ting-hua Wang |
| author_facet | Xiao-li Min Ting-yong Wang Yi Cao Jia Liu Jin-tao Li Ting-hua Wang |
| author_sort | Xiao-li Min |
| collection | DOAJ |
| description | To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function classifications, as well as the signaling pathways of genes targeted by these differentially expressed miRNAs. Our results show significantly changed miRNA expression profiles in the reperfusion period after focal cerebral ischemia, with a total of 15 miRNAs up-regulated and 44 miRNAs down-regulated. Target genes of these differentially expressed miRNAs were mainly involved in metabolic and cellular processes, which were identified as hub nodes of a miRNA-GO-network. The most correlated pathways included D-glutamine and D-glutamate metabolism, the renin-angiotensin system, peroxisomes, the PPAR signaling pathway, SNARE interactions in vesicular transport, and the calcium signaling pathway. Our study suggests that miRNAs play an important role in the pathological process of cerebral ischemia/reperfusion injury. Understanding miRNA expression and function may shed light on the molecular mechanism of cerebral ischemia/reperfusion injury. |
| first_indexed | 2024-12-19T06:11:54Z |
| format | Article |
| id | doaj.art-d50c596dee9a42a099d73e84caedd848 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-19T06:11:54Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-d50c596dee9a42a099d73e84caedd8482022-12-21T20:33:00ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110111799180810.4103/1673-5374.170302MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?Xiao-li MinTing-yong WangYi CaoJia LiuJin-tao LiTing-hua WangTo determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function classifications, as well as the signaling pathways of genes targeted by these differentially expressed miRNAs. Our results show significantly changed miRNA expression profiles in the reperfusion period after focal cerebral ischemia, with a total of 15 miRNAs up-regulated and 44 miRNAs down-regulated. Target genes of these differentially expressed miRNAs were mainly involved in metabolic and cellular processes, which were identified as hub nodes of a miRNA-GO-network. The most correlated pathways included D-glutamine and D-glutamate metabolism, the renin-angiotensin system, peroxisomes, the PPAR signaling pathway, SNARE interactions in vesicular transport, and the calcium signaling pathway. Our study suggests that miRNAs play an important role in the pathological process of cerebral ischemia/reperfusion injury. Understanding miRNA expression and function may shed light on the molecular mechanism of cerebral ischemia/reperfusion injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=11;spage=1799;epage=1808;aulast=Minnerve regeneration; microRNA; therapeutic target; cerebral ischemia/reperfusion injury; miRNA expression profiles; bioinformatics analysis; Gene Ontology analysis; molecular mechanism; KEGG pathway; neural regeneration |
| spellingShingle | Xiao-li Min Ting-yong Wang Yi Cao Jia Liu Jin-tao Li Ting-hua Wang MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? Neural Regeneration Research nerve regeneration; microRNA; therapeutic target; cerebral ischemia/reperfusion injury; miRNA expression profiles; bioinformatics analysis; Gene Ontology analysis; molecular mechanism; KEGG pathway; neural regeneration |
| title | MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? |
| title_full | MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? |
| title_fullStr | MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? |
| title_full_unstemmed | MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? |
| title_short | MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? |
| title_sort | micrornas a novel promising therapeutic target for cerebral ischemia reperfusion injury |
| topic | nerve regeneration; microRNA; therapeutic target; cerebral ischemia/reperfusion injury; miRNA expression profiles; bioinformatics analysis; Gene Ontology analysis; molecular mechanism; KEGG pathway; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=11;spage=1799;epage=1808;aulast=Min |
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