Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients
Background and Aim: The most common form of primary liver cancer in adults is hepatocellular carcinoma (HCC), and it is the most common cause of death in people with cirrhosis. The mammalian Sir2 family or sirtuins is formed of a cluster of highly conserved proteins triggering nicotinamide adenine d...
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| Format: | Article |
| Language: | English |
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Beni-Suef University, Faculty of Medicine
2022-04-01
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| Series: | Egyptian Journal of Medical Research |
| Subjects: | |
| Online Access: | https://ejmr.journals.ekb.eg/article_239081_f30c13d1c281d323b52773d970935c02.pdf |
| _version_ | 1828003671635394560 |
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| author | Ahmed Ibrahim Laila Rashed Antoun Ghaly Mohamed Ahmed |
| author_facet | Ahmed Ibrahim Laila Rashed Antoun Ghaly Mohamed Ahmed |
| author_sort | Ahmed Ibrahim |
| collection | DOAJ |
| description | Background and Aim: The most common form of primary liver cancer in adults is hepatocellular carcinoma (HCC), and it is the most common cause of death in people with cirrhosis. The mammalian Sir2 family or sirtuins is formed of a cluster of highly conserved proteins triggering nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase activity which target histone and non-histone substrates including enzymes, transcription regulators, tumor suppressors, cell signaling proteins and DNA repair proteins. Seven human sirtuins have been identified and named SIRT1-7. SIRT1 has been regarded as a tumor promoter due to its increased expression in some types of cancers and its role in the inactivation of proteins involved in tumor suppression and DNA damage repair.We aimed to estimate the level of SIRT-1 in the blood of HBV, HCV, and HCC patients, to investigate the possible role of SIRT-1 in the pathogenesis of HCC and to determine the role of SIRT-1 as a tumor marker or pomoter compared to other markers like α-feto protein. Subjects and Methods: This study was performed in Internal Medicine department Beni-Suef Hospital. Our study included 160 individuals; divided into four groups; group A (healthy control), group B (HBV patients), group C (HCV patients) and group D (HCC patients). The serum level of SIRT-1 was assessed and compared in all groups and correlate its level as a tumor marker with the other marker α-fetoprotein. Results: There was significant increase in SIRT-1 levels in HBV, HCV and HCC group as compared to control (p value <0.001). There was significant increase in SIRT-1 in HCV and HCC groups as compared to HBV group (p value <0.001). There was significant increase in SIRT-1 levels in HCC group as compared to HCV group (p value <0.001). There was positive correlation between α - feto protein and SIRT-1in HCC group only but no correlation in others groups. Conclusion: Our study demonstrated that SIRT-1 is overexpressed in HCC patients compared to chronic noncancerous viral liver parenchyma diseases, so it may be included in pathogenesis of HCC. SIRT-1 may have matchable specificity and sensitivity as AFP in HCC patients. |
| first_indexed | 2024-04-10T07:02:08Z |
| format | Article |
| id | doaj.art-d50cab9af1e54d5d802ad9fddfb91120 |
| institution | Directory Open Access Journal |
| issn | 2682-4396 2682-440X |
| language | English |
| last_indexed | 2024-04-10T07:02:08Z |
| publishDate | 2022-04-01 |
| publisher | Beni-Suef University, Faculty of Medicine |
| record_format | Article |
| series | Egyptian Journal of Medical Research |
| spelling | doaj.art-d50cab9af1e54d5d802ad9fddfb911202023-02-27T17:41:48ZengBeni-Suef University, Faculty of MedicineEgyptian Journal of Medical Research2682-43962682-440X2022-04-013217018410.21608/ejmr.2022.239081239081Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma PatientsAhmed Ibrahim0Laila Rashed1Antoun Ghaly2Mohamed Ahmed3Department of Internal medicine, Faculty of Medicine, Beni-Suef University, EgyptDepartment of Biochemistry, Faculty of Medicine, Cairo University, EgyptDepartment of Internal medicine, One-day surgery Hospital, Minia, EgyptDepartment of Internal medicine, Faculty of Medicine, Beni-Suef University, EgyptBackground and Aim: The most common form of primary liver cancer in adults is hepatocellular carcinoma (HCC), and it is the most common cause of death in people with cirrhosis. The mammalian Sir2 family or sirtuins is formed of a cluster of highly conserved proteins triggering nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase activity which target histone and non-histone substrates including enzymes, transcription regulators, tumor suppressors, cell signaling proteins and DNA repair proteins. Seven human sirtuins have been identified and named SIRT1-7. SIRT1 has been regarded as a tumor promoter due to its increased expression in some types of cancers and its role in the inactivation of proteins involved in tumor suppression and DNA damage repair.We aimed to estimate the level of SIRT-1 in the blood of HBV, HCV, and HCC patients, to investigate the possible role of SIRT-1 in the pathogenesis of HCC and to determine the role of SIRT-1 as a tumor marker or pomoter compared to other markers like α-feto protein. Subjects and Methods: This study was performed in Internal Medicine department Beni-Suef Hospital. Our study included 160 individuals; divided into four groups; group A (healthy control), group B (HBV patients), group C (HCV patients) and group D (HCC patients). The serum level of SIRT-1 was assessed and compared in all groups and correlate its level as a tumor marker with the other marker α-fetoprotein. Results: There was significant increase in SIRT-1 levels in HBV, HCV and HCC group as compared to control (p value <0.001). There was significant increase in SIRT-1 in HCV and HCC groups as compared to HBV group (p value <0.001). There was significant increase in SIRT-1 levels in HCC group as compared to HCV group (p value <0.001). There was positive correlation between α - feto protein and SIRT-1in HCC group only but no correlation in others groups. Conclusion: Our study demonstrated that SIRT-1 is overexpressed in HCC patients compared to chronic noncancerous viral liver parenchyma diseases, so it may be included in pathogenesis of HCC. SIRT-1 may have matchable specificity and sensitivity as AFP in HCC patients.https://ejmr.journals.ekb.eg/article_239081_f30c13d1c281d323b52773d970935c02.pdfhcchbvhcvsirt-1 |
| spellingShingle | Ahmed Ibrahim Laila Rashed Antoun Ghaly Mohamed Ahmed Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients Egyptian Journal of Medical Research hcc hbv hcv sirt-1 |
| title | Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients |
| title_full | Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients |
| title_fullStr | Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients |
| title_full_unstemmed | Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients |
| title_short | Comparative Study of SIRT1 Expression in Hepatitis B Virus, Hepatitis C Virus, and Hepatocellular Carcinoma Patients |
| title_sort | comparative study of sirt1 expression in hepatitis b virus hepatitis c virus and hepatocellular carcinoma patients |
| topic | hcc hbv hcv sirt-1 |
| url | https://ejmr.journals.ekb.eg/article_239081_f30c13d1c281d323b52773d970935c02.pdf |
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