First detection of dengue virus in the saliva of immunocompetent murine model
The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isol...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2018-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400400&lng=en&tlng=en |
| _version_ | 1797758533424381952 |
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| author | Arthur da Costa Rasinhas Marcos Alexandre Nunes da Silva Gabriela Cardoso Caldas Fernanda Cunha Jácome Raphael Leonardo Flávia Barreto dos Santos Priscila Conrado Guerra Nunes Ortrud Monika Barth Debora Ferreira Barreto-Vieira |
| author_facet | Arthur da Costa Rasinhas Marcos Alexandre Nunes da Silva Gabriela Cardoso Caldas Fernanda Cunha Jácome Raphael Leonardo Flávia Barreto dos Santos Priscila Conrado Guerra Nunes Ortrud Monika Barth Debora Ferreira Barreto-Vieira |
| author_sort | Arthur da Costa Rasinhas |
| collection | DOAJ |
| description | The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4. |
| first_indexed | 2024-03-12T18:31:23Z |
| format | Article |
| id | doaj.art-d50cd74e398b4a3b9d1636b9a82046e0 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:31:23Z |
| publishDate | 2018-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d50cd74e398b4a3b9d1636b9a82046e02023-08-02T08:19:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602018-02-01113410.1590/0074-02760170208S0074-02762018000400400First detection of dengue virus in the saliva of immunocompetent murine modelArthur da Costa RasinhasMarcos Alexandre Nunes da SilvaGabriela Cardoso CaldasFernanda Cunha JácomeRaphael LeonardoFlávia Barreto dos SantosPriscila Conrado Guerra NunesOrtrud Monika BarthDebora Ferreira Barreto-VieiraThe lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400400&lng=en&tlng=endengue 4salivaBALB/c miceimmunocompetent murine model |
| spellingShingle | Arthur da Costa Rasinhas Marcos Alexandre Nunes da Silva Gabriela Cardoso Caldas Fernanda Cunha Jácome Raphael Leonardo Flávia Barreto dos Santos Priscila Conrado Guerra Nunes Ortrud Monika Barth Debora Ferreira Barreto-Vieira First detection of dengue virus in the saliva of immunocompetent murine model Memorias do Instituto Oswaldo Cruz dengue 4 saliva BALB/c mice immunocompetent murine model |
| title | First detection of dengue virus in the saliva of immunocompetent murine model |
| title_full | First detection of dengue virus in the saliva of immunocompetent murine model |
| title_fullStr | First detection of dengue virus in the saliva of immunocompetent murine model |
| title_full_unstemmed | First detection of dengue virus in the saliva of immunocompetent murine model |
| title_short | First detection of dengue virus in the saliva of immunocompetent murine model |
| title_sort | first detection of dengue virus in the saliva of immunocompetent murine model |
| topic | dengue 4 saliva BALB/c mice immunocompetent murine model |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400400&lng=en&tlng=en |
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