A chorismate mutase from Radopholus similis plays an essential role in pathogenicity
In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study, based on transcriptome data, a chorismate mutase gene of Radoph...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923001107 |
| _version_ | 1797261869311852544 |
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| author | Sihua Yang Junyi Li Shuai Yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie |
| author_facet | Sihua Yang Junyi Li Shuai Yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie |
| author_sort | Sihua Yang |
| collection | DOAJ |
| description | In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study, based on transcriptome data, a chorismate mutase gene of Radopholus similis (RsCM) was identified and cloned, which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity (PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference (RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid (SA) pathway genes (PR1 and PR5) in roots infected by the nematode were significantly down-regulated, which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes. |
| first_indexed | 2024-04-24T23:48:04Z |
| format | Article |
| id | doaj.art-d5118e0c3e344dbd90e04a10294d907e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-24T23:48:04Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d5118e0c3e344dbd90e04a10294d907e2024-03-15T04:43:08ZengElsevierJournal of Integrative Agriculture2095-31192024-03-01233923937A chorismate mutase from Radopholus similis plays an essential role in pathogenicitySihua Yang0Junyi Li1Shuai Yang2Shiqiao Tang3Huizhong Wang4Chunling Xu5Hui Xie6Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaLaboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaLaboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaLaboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaLaboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCorrespondence Chunling Xu; Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCorrespondence Hui Xie; Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaIn the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study, based on transcriptome data, a chorismate mutase gene of Radopholus similis (RsCM) was identified and cloned, which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity (PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference (RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid (SA) pathway genes (PR1 and PR5) in roots infected by the nematode were significantly down-regulated, which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.http://www.sciencedirect.com/science/article/pii/S2095311923001107Radopholus similischorismate mutaseplant defensetransgenic tomato |
| spellingShingle | Sihua Yang Junyi Li Shuai Yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie A chorismate mutase from Radopholus similis plays an essential role in pathogenicity Journal of Integrative Agriculture Radopholus similis chorismate mutase plant defense transgenic tomato |
| title | A chorismate mutase from Radopholus similis plays an essential role in pathogenicity |
| title_full | A chorismate mutase from Radopholus similis plays an essential role in pathogenicity |
| title_fullStr | A chorismate mutase from Radopholus similis plays an essential role in pathogenicity |
| title_full_unstemmed | A chorismate mutase from Radopholus similis plays an essential role in pathogenicity |
| title_short | A chorismate mutase from Radopholus similis plays an essential role in pathogenicity |
| title_sort | chorismate mutase from radopholus similis plays an essential role in pathogenicity |
| topic | Radopholus similis chorismate mutase plant defense transgenic tomato |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923001107 |
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