Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers
Despite the general use of endoxylanases in poultry feed to improve broiler performance, the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo. To gain insight into this functio...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-09-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654521000603 |
| _version_ | 1818361850244366336 |
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| author | An Bautil Johan Buyse Peter Goos Michael R. Bedford Christophe M. Courtin |
| author_facet | An Bautil Johan Buyse Peter Goos Michael R. Bedford Christophe M. Courtin |
| author_sort | An Bautil |
| collection | DOAJ |
| description | Despite the general use of endoxylanases in poultry feed to improve broiler performance, the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo. To gain insight into this functionality, we investigated the impact of endoxylanase type (Belfeed from Bacillus subtilis versus Econase XT from Nonomuraea flexuosa) and dose (10, 100, 1,000 mg/kg) in combination with broiler age on arabinoxylan (AX) hydrolysis and fermentation in broilers (Ross 308) fed a wheat-soy based diet. In a digestibility trial and a performance trial, a total of 1,057 one-day-old chicks received the control diet or 1 of the 6 endoxylanase supplemented wheat-soy based diets with, respectively, 5 replicate cages and 8 replicate pens per dietary treatment per trial. The AX content and structure, the AX digestibility values and the short-chain fatty acids produced were analysed at the level of the ileum, caeca and excreta at d 11 and 36. Endoxylanase supplementation resulted in a more extensive solubilisation of wheat AX and a reduction in the intestinal viscosity compared to the control (P < 0.05). A high endoxylanase dose was, however, required to obtain increased hydrolysis of the dietary AX along the gastrointestinal tract against the control (P < 0.001). Depending on the type of endoxylanase, a pool of AX with distinct physicochemical properties was created. The B. subtilis endoxylanase created a large pool of soluble AX in the ileum, thereby increasing ileal viscosity compared to broilers fed an endoxylanase from N. flexuosa (P < 0.001). The N. flexuosa endoxylanase mainly triggered caecal AX fermentation in young broilers, by delivering easily fermentable AX substrates with a low degree of polymerisation (P = 0.03). The effects were particularly present in young broilers (d 11). From this study, it is clear that the type and dose of endoxylanase added to wheat-soy based diets determine the nature of AX substrates formed. These, in turn, affect the intestinal viscosity and the interplay between the dietary AX compounds and microbiota, hence dictating AX digestion at young broiler ages and performance outcomes towards slaughter age. |
| first_indexed | 2024-12-13T21:23:14Z |
| format | Article |
| id | doaj.art-d511b729e82d4857ae15aeae544fdcc3 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-12-13T21:23:14Z |
| publishDate | 2021-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-d511b729e82d4857ae15aeae544fdcc32022-12-21T23:31:02ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452021-09-0173787800Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilersAn Bautil0Johan Buyse1Peter Goos2Michael R. Bedford3Christophe M. Courtin4Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems (M2S), KU Leuven, 3001, Leuven, BelgiumLaboratory of Livestock Physiology, Department of Biosystems, KU Leuven, 3001, Leuven, BelgiumMeBioS Division, Department of Biosystems, KU Leuven, 3001, Leuven, BelgiumAB Vista, Marlborough, Wiltshire, SN8 4AN, United KingdomLaboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems (M2S), KU Leuven, 3001, Leuven, Belgium; Corresponding author.Despite the general use of endoxylanases in poultry feed to improve broiler performance, the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo. To gain insight into this functionality, we investigated the impact of endoxylanase type (Belfeed from Bacillus subtilis versus Econase XT from Nonomuraea flexuosa) and dose (10, 100, 1,000 mg/kg) in combination with broiler age on arabinoxylan (AX) hydrolysis and fermentation in broilers (Ross 308) fed a wheat-soy based diet. In a digestibility trial and a performance trial, a total of 1,057 one-day-old chicks received the control diet or 1 of the 6 endoxylanase supplemented wheat-soy based diets with, respectively, 5 replicate cages and 8 replicate pens per dietary treatment per trial. The AX content and structure, the AX digestibility values and the short-chain fatty acids produced were analysed at the level of the ileum, caeca and excreta at d 11 and 36. Endoxylanase supplementation resulted in a more extensive solubilisation of wheat AX and a reduction in the intestinal viscosity compared to the control (P < 0.05). A high endoxylanase dose was, however, required to obtain increased hydrolysis of the dietary AX along the gastrointestinal tract against the control (P < 0.001). Depending on the type of endoxylanase, a pool of AX with distinct physicochemical properties was created. The B. subtilis endoxylanase created a large pool of soluble AX in the ileum, thereby increasing ileal viscosity compared to broilers fed an endoxylanase from N. flexuosa (P < 0.001). The N. flexuosa endoxylanase mainly triggered caecal AX fermentation in young broilers, by delivering easily fermentable AX substrates with a low degree of polymerisation (P = 0.03). The effects were particularly present in young broilers (d 11). From this study, it is clear that the type and dose of endoxylanase added to wheat-soy based diets determine the nature of AX substrates formed. These, in turn, affect the intestinal viscosity and the interplay between the dietary AX compounds and microbiota, hence dictating AX digestion at young broiler ages and performance outcomes towards slaughter age.http://www.sciencedirect.com/science/article/pii/S2405654521000603BroilerArabinoxylanFeed enzymeEndoxylanaseDigestion |
| spellingShingle | An Bautil Johan Buyse Peter Goos Michael R. Bedford Christophe M. Courtin Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers Animal Nutrition Broiler Arabinoxylan Feed enzyme Endoxylanase Digestion |
| title | Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| title_full | Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| title_fullStr | Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| title_full_unstemmed | Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| title_short | Feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| title_sort | feed endoxylanase type and dose affect arabinoxylan hydrolysis and fermentation in ageing broilers |
| topic | Broiler Arabinoxylan Feed enzyme Endoxylanase Digestion |
| url | http://www.sciencedirect.com/science/article/pii/S2405654521000603 |
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