Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato
The starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quan...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00914/full |
| _version_ | 1811278945402748928 |
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| author | Kai Zhang Kai Zhang Kai Zhang Zhengdan Wu Zhengdan Wu Daobin Tang Daobin Tang Daobin Tang Kai Luo Kai Luo Huixiang Lu Huixiang Lu Yingying Liu Yingying Liu Jie Dong Jie Dong Xin Wang Xin Wang Changwen Lv Changwen Lv Changwen Lv Jichun Wang Jichun Wang Jichun Wang Kun Lu Kun Lu |
| author_facet | Kai Zhang Kai Zhang Kai Zhang Zhengdan Wu Zhengdan Wu Daobin Tang Daobin Tang Daobin Tang Kai Luo Kai Luo Huixiang Lu Huixiang Lu Yingying Liu Yingying Liu Jie Dong Jie Dong Xin Wang Xin Wang Changwen Lv Changwen Lv Changwen Lv Jichun Wang Jichun Wang Jichun Wang Kun Lu Kun Lu |
| author_sort | Kai Zhang |
| collection | DOAJ |
| description | The starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quantified. In this study, we measured the starch and sugar contents and analyzed the transcriptome profiles of SRs harvested from sweet potatoes with high, medium, and extremely low starch contents, at five developmental stages [65, 80, 95, 110, and 125 days after transplanting (DAP)]. We found that differences in both water content and starch accumulation in the dry matter affect the starch content of SRs in different sweet potato genotypes. Based on transcriptome sequencing data, we assembled 112336 unigenes, and identified several differentially expressed genes (DEGs) involved in starch and sucrose metabolism, and revealed the transcriptional regulatory network controlling starch and sucrose metabolism in sweet potato SRs. Correlation analysis between expression patterns and starch and sugar contents suggested that the sugar–starch conversion steps catalyzed by sucrose synthase (SuSy) and UDP-glucose pyrophosphorylase (UGPase) may be essential for starch accumulation in the dry matter of SRs, and IbβFRUCT2, a vacuolar acid invertase, might also be a key regulator of starch content in the SRs. Our results provide valuable resources for future investigations aimed at deciphering the molecular mechanisms determining the starch properties of sweet potato SRs. |
| first_indexed | 2024-04-13T00:44:51Z |
| format | Article |
| id | doaj.art-d51381718fc845388797e52ceb1b460e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T00:44:51Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d51381718fc845388797e52ceb1b460e2022-12-22T03:10:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.00914235917Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet PotatoKai Zhang0Kai Zhang1Kai Zhang2Zhengdan Wu3Zhengdan Wu4Daobin Tang5Daobin Tang6Daobin Tang7Kai Luo8Kai Luo9Huixiang Lu10Huixiang Lu11Yingying Liu12Yingying Liu13Jie Dong14Jie Dong15Xin Wang16Xin Wang17Changwen Lv18Changwen Lv19Changwen Lv20Jichun Wang21Jichun Wang22Jichun Wang23Kun Lu24Kun Lu25College of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaEngineering Research Center of South Upland Agriculture, Ministry of Education, Southwest UniversityBeibei, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaEngineering Research Center of South Upland Agriculture, Ministry of Education, Southwest UniversityBeibei, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaEngineering Research Center of South Upland Agriculture, Ministry of Education, Southwest UniversityBeibei, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaEngineering Research Center of South Upland Agriculture, Ministry of Education, Southwest UniversityBeibei, ChinaSweet Potato Engineering and Technology Research CenterChongqing, ChinaCollege of Agronomy and Biotechnology, Southwest UniversityChongqing, ChinaEngineering Research Center of South Upland Agriculture, Ministry of Education, Southwest UniversityBeibei, ChinaThe starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quantified. In this study, we measured the starch and sugar contents and analyzed the transcriptome profiles of SRs harvested from sweet potatoes with high, medium, and extremely low starch contents, at five developmental stages [65, 80, 95, 110, and 125 days after transplanting (DAP)]. We found that differences in both water content and starch accumulation in the dry matter affect the starch content of SRs in different sweet potato genotypes. Based on transcriptome sequencing data, we assembled 112336 unigenes, and identified several differentially expressed genes (DEGs) involved in starch and sucrose metabolism, and revealed the transcriptional regulatory network controlling starch and sucrose metabolism in sweet potato SRs. Correlation analysis between expression patterns and starch and sugar contents suggested that the sugar–starch conversion steps catalyzed by sucrose synthase (SuSy) and UDP-glucose pyrophosphorylase (UGPase) may be essential for starch accumulation in the dry matter of SRs, and IbβFRUCT2, a vacuolar acid invertase, might also be a key regulator of starch content in the SRs. Our results provide valuable resources for future investigations aimed at deciphering the molecular mechanisms determining the starch properties of sweet potato SRs.http://journal.frontiersin.org/article/10.3389/fpls.2017.00914/fullexpression profileRNA-seqstarchstorage rootsucrosesweet potato |
| spellingShingle | Kai Zhang Kai Zhang Kai Zhang Zhengdan Wu Zhengdan Wu Daobin Tang Daobin Tang Daobin Tang Kai Luo Kai Luo Huixiang Lu Huixiang Lu Yingying Liu Yingying Liu Jie Dong Jie Dong Xin Wang Xin Wang Changwen Lv Changwen Lv Changwen Lv Jichun Wang Jichun Wang Jichun Wang Kun Lu Kun Lu Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato Frontiers in Plant Science expression profile RNA-seq starch storage root sucrose sweet potato |
| title | Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato |
| title_full | Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato |
| title_fullStr | Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato |
| title_short | Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato |
| title_sort | comparative transcriptome analysis reveals critical function of sucrose metabolism related enzymes in starch accumulation in the storage root of sweet potato |
| topic | expression profile RNA-seq starch storage root sucrose sweet potato |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00914/full |
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