Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins.
Nascent high density lipoprotein (HDL) and nascent very low density lipoprotein (VLDL) were isolated from rat livers that had been perfused with [3H]glycerol to label the triglyceride. When injected into intact rats, the labeled HDL-triglyceride disappeared as rapidly as the VLDL-triglyceride, with...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1989-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520383012 |
| _version_ | 1818383756670533632 |
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| author | K E Winkler J B Marsh |
| author_facet | K E Winkler J B Marsh |
| author_sort | K E Winkler |
| collection | DOAJ |
| description | Nascent high density lipoprotein (HDL) and nascent very low density lipoprotein (VLDL) were isolated from rat livers that had been perfused with [3H]glycerol to label the triglyceride. When injected into intact rats, the labeled HDL-triglyceride disappeared as rapidly as the VLDL-triglyceride, with only 10% of the injected label remaining in the plasma after 30 min. The protein moiety of nascent HDL was labeled with [35S]methionine in a similar fashion and the labeled nascent HDL was separated into nonretained (NR) and retained (R) fractions by heparin-Sepharose affinity chromatography. When injected into rats, 55% of the injected label in nascent fraction NR and 72% of that in nascent fraction R was recovered from plasma at 30 min, compared to only 10% of the triglyceride label from unfractionated nascent HDL, indicating dissociation of triglyceride and apolipoprotein clearance. The plasma decay curves for both triglyceride and protein were biexponential. By 5 min, 15% of the 35S label remaining in plasma represented apoE and apoC that had been transferred from nascent HDL fractions NR and R to the d less than 1.063 g/ml fraction of plasma. Plasma HDL was labeled in vivo with [35S]methionine, separated into fractions NR and R, and the clearance of the two plasma HDL fractions was compared with that of the corresponding nascent HDL fractions. Except for a faster rate of removal of the nascent HDL fractions during the first 5 min, the serum decay curves were very similar.(ABSTRACT TRUNCATED AT 250 WORDS) |
| first_indexed | 2024-12-14T03:11:26Z |
| format | Article |
| id | doaj.art-d5155caf24804194b0e7de89d8d19206 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:11:26Z |
| publishDate | 1989-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-d5155caf24804194b0e7de89d8d192062022-12-21T23:19:16ZengElsevierJournal of Lipid Research0022-22751989-07-01307989996Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins.K E Winkler0J B Marsh1Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.Nascent high density lipoprotein (HDL) and nascent very low density lipoprotein (VLDL) were isolated from rat livers that had been perfused with [3H]glycerol to label the triglyceride. When injected into intact rats, the labeled HDL-triglyceride disappeared as rapidly as the VLDL-triglyceride, with only 10% of the injected label remaining in the plasma after 30 min. The protein moiety of nascent HDL was labeled with [35S]methionine in a similar fashion and the labeled nascent HDL was separated into nonretained (NR) and retained (R) fractions by heparin-Sepharose affinity chromatography. When injected into rats, 55% of the injected label in nascent fraction NR and 72% of that in nascent fraction R was recovered from plasma at 30 min, compared to only 10% of the triglyceride label from unfractionated nascent HDL, indicating dissociation of triglyceride and apolipoprotein clearance. The plasma decay curves for both triglyceride and protein were biexponential. By 5 min, 15% of the 35S label remaining in plasma represented apoE and apoC that had been transferred from nascent HDL fractions NR and R to the d less than 1.063 g/ml fraction of plasma. Plasma HDL was labeled in vivo with [35S]methionine, separated into fractions NR and R, and the clearance of the two plasma HDL fractions was compared with that of the corresponding nascent HDL fractions. Except for a faster rate of removal of the nascent HDL fractions during the first 5 min, the serum decay curves were very similar.(ABSTRACT TRUNCATED AT 250 WORDS)http://www.sciencedirect.com/science/article/pii/S0022227520383012 |
| spellingShingle | K E Winkler J B Marsh Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. Journal of Lipid Research |
| title | Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. |
| title_full | Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. |
| title_fullStr | Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. |
| title_full_unstemmed | Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. |
| title_short | Metabolism of triglyceride-rich nascent rat hepatic high density lipoproteins. |
| title_sort | metabolism of triglyceride rich nascent rat hepatic high density lipoproteins |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520383012 |
| work_keys_str_mv | AT kewinkler metabolismoftriglyceriderichnascentrathepatichighdensitylipoproteins AT jbmarsh metabolismoftriglyceriderichnascentrathepatichighdensitylipoproteins |