Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase.

Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase.

The enzyme dihydrodipicolinate reductase (DHDPR) is a component of the lysine biosynthetic pathway in bacteria and higher plants. DHDPR catalyzes the NAD(P)H dependent reduction of 2,3-dihydrodipicolinate to the cyclic imine L-2,3,4,5,-tetrahydropicolinic acid. The dapB gene that encodes dihydrodipi...

Full description

Bibliographic Details
Main Authors:	Yvonne D Trigoso, Russell C Evans, William E Karsten, Lilian Chooback
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2016-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC4729673?pdf=render

Similar Items

Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase and dihydrodipicolinate reductase in lactobacillus plantarum
by: , Muhammad N. Cahyaynto, t Jiroko Kawasaki, mariko Nagashio, et al.
Published: (2007)

Repurposed inhibitor of bacterial dihydrodipicolinate reductase exhibits effective herbicidal activity
by: Emily R. R. Mackie, et al.
Published: (2023-05-01)

Cloning of lactobacillus plantarum IAM 12477 lysine biosynthetic genes encoding functional aspatate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydropicolinate reductase
by: , Muhammad Nur Cahyanto, et al.
Published: (2006)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.
by: Trikkur Madom Seetaraman Amritha, et al.
Published: (2020-01-01)

Expression and Purification of PhoR Sensor-Domain Histidine Kinase of Mycobacterium tuberculosis in Escherichia coli
by: ERNAWATI ARIFIN GIRI-RACHMAN, et al.
Published: (2015-11-01)

Cloning of flaving reductase gene of Citrobacter freundi A1 into Escherichia coli /
by: Narges Janalizadeh, 1983-, et al.
Published: (2010)

Tagging the expressed protein with 6 histidines: rapid cloning of an amplicon with three options.
by: Manika Indrajit Singh, et al.
Published: (2013-01-01)

Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in <i>Escherichia coli</i>, Purification, and Characterization
by: Monika Cserjan-Puschmann, et al.
Published: (2020-11-01)

Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli
by: Meraj Pourhossein, et al.
Published: (2014-01-01)

Cloning, expression and purification of fructosyl amino acid oxidase (FAOX) in Escherichia Coli
by: Ho Ta Giap, et al.
Published: (2021-03-01)

High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG.
by: Dan Luo, et al.
Published: (2016-01-01)

Cloning of flaving reductase gene of Citrobacter freundi A1 into Escherichia coli [electronic resource] /
by: Narges Janalizadeh, 1983-, et al.
Published: (2010)

Purification of histidine-tagged nucleocapsid protein of Nipah virus using immobilized metal affinity chromatography
by: Fui , Chin Chong, et al.
Published: (2009)

Synthesis of Ni2+-functionalized polydopamine magnetic beads for facilitated purification of histidine-tagged proteins
by: Alireza Shariati, et al.
Published: (2023-10-01)

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli
by: L. Avilán, et al.
Published: (1997-12-01)

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: The novel Fh8 system
by: Sofia eCosta, et al.
Published: (2014-02-01)

Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant buforin I expressed in periplasmic space of Escherichia coli
by: Sahar Roshanak, et al.
Published: (2023-04-01)

In silico virtual screening for the identification of novel inhibitors against dihydrodipicolinate reductase (DapB) of Mycobacterium tuberculosis, a key enzyme of diaminopimelate pathway
by: Nupur Angrish, et al.
Published: (2023-12-01)

Tetrahydrodipicolinate N-succinyltransferase and dihydrodipicolinate synthase from Pseudomonas aeruginosa: structure analysis and gene deletion.
by: Robert Schnell, et al.
Published: (2012-01-01)

Purification and Characterization of Endoxylanases Cloned from Fibrobacter Succinogenes S85 and Expressed in Escherichia Coli HB101
by: Soong, Chee Leong
Published: (1996)

Ultrafast purification and reconstitution of His-tagged cysteine-less Escherichia coli F1Fo ATP synthase.
by: Ishmukhametov, R, et al.
Published: (2005)

Ultrafast purification and reconstitution of His-tagged cysteine-less Escherichia coli F1Fo ATP synthase
by: Ishmukhametov, RR, et al.
Published: (2004)

Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli
by: Tyler L. Dangerfield, et al.
Published: (2021-03-01)

Histidine hydrogen-deuterium exchange mass spectrometry for probing the microenvironment of histidine residues in dihydrofolate reductase.
by: Masaru Miyagi, et al.
Published: (2011-02-01)

Expression and Purification of PhoR Sensor-Domain Histidine Kinase of Mycobacterium tuberculosis in Eschericia coli
by: ERNAWATI ARIFIN GIRI-RACHMAN, et al.
Published: (2015-06-01)

Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli
by: Kirsten Lehotsky, et al.
Published: (2024-06-01)

Structural and dynamic requirements for optimal activity of the essential bacterial enzyme dihydrodipicolinate synthase.
by: C F Reboul, et al.
Published: (2012-01-01)

Crystal, solution and in silico structural studies of dihydrodipicolinate synthase from the common grapevine.
by: Sarah C Atkinson, et al.
Published: (2012-01-01)

Cloning and expression of Epstein-Barr viral antigens in Escherichia coli : purification of recombinant EBV antigens for diagnostic application
by: Fones-Tan, Angela.
Published: (2009)

Cloning, optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli
by: Mitra Ashayeri-Panah, et al.
Published: (2017-08-01)

Cloning, Expression, and Purification of α, βa, and βb Subunits of Human Inhibin Protein in Escherichia coli
by: Mohammad Ataei, et al.
Published: (2018-07-01)

Poly-()-histidine immobilized cryogels for lysozyme purification
by: Duygu Çimen, et al.
Published: (2016-10-01)

Modification in Toxicity of l‑Histidine-Incorporated ZnO Nanoparticles toward Escherichia coli
by: Shraddha Mahakal, et al.
Published: (2023-09-01)

The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli cells.
by: Erik Sommer, et al.
Published: (2013-01-01)

Effect of Biochar on the Production of L-Histidine From Glucose Through Escherichia coli Metabolism
by: Yang E, et al.
Published: (2021-01-01)

Expression and purification of recombinant neurotensin in Escherichia coli.
by: Williamson, P, et al.
Published: (2000)

Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase
by: Ando, Nozomi, et al.
Published: (2012)

Proton-coupled electron transfer in the Escherichia coli ribonucleotide reductase
by: Chang, Michelle C. Y. (Michelle Chia-yu), 1977-
Published: (2006)

Biofilm modifies expression of ribonucleotide reductase genes in Escherichia coli.
by: Maria del Mar Cendra, et al.
Published: (2012-01-01)

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
by: D. Molinková
Published: (2001-02-01)