Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites
Summary: Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of ba...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222013529 |
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| author | Alejandro Gómez-Mejia Kim Arnold Julian Bär Kapil Dev Singh Thomas C. Scheier Silvio D. Brugger Annelies S. Zinkernagel Pablo Sinues |
| author_facet | Alejandro Gómez-Mejia Kim Arnold Julian Bär Kapil Dev Singh Thomas C. Scheier Silvio D. Brugger Annelies S. Zinkernagel Pablo Sinues |
| author_sort | Alejandro Gómez-Mejia |
| collection | DOAJ |
| description | Summary: Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state-of-the-art diagnostics. Volatile organic compounds (VOCs) produced by growing S. aureus or S. pneumoniae cultures on blood agar plates were detected within minutes and allowed for the distinction of these two bacteria on a species and even strain level within hours. Furthermore, we obtained a fingerprint of clinical patient samples within minutes of measurement and predominantly observed a separation of samples containing live bacteria compared to samples with no bacterial growth. Further development of this technique may reduce the time required for microbiological diagnosis and should help to improve patient’s tailored treatment. |
| first_indexed | 2024-04-12T20:59:35Z |
| format | Article |
| id | doaj.art-d517f166178445c39d2815a0269bb019 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T20:59:35Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d517f166178445c39d2815a0269bb0192022-12-22T03:16:52ZengElsevieriScience2589-00422022-10-012510105080Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolitesAlejandro Gómez-Mejia0Kim Arnold1Julian Bär2Kapil Dev Singh3Thomas C. Scheier4Silvio D. Brugger5Annelies S. Zinkernagel6Pablo Sinues7Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, 8091 Zurich, SwitzerlandUniversity Children’s Hospital Basel (UKBB), 4056 Basel, Switzerland; Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandDepartment of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, 8091 Zurich, SwitzerlandUniversity Children’s Hospital Basel (UKBB), 4056 Basel, Switzerland; Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandDepartment of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, 8091 Zurich, SwitzerlandDepartment of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, 8091 Zurich, SwitzerlandDepartment of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, 8091 Zurich, Switzerland; Corresponding authorUniversity Children’s Hospital Basel (UKBB), 4056 Basel, Switzerland; Department of Biomedical Engineering, University of Basel, 4123 Allschwil, Switzerland; Corresponding authorSummary: Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state-of-the-art diagnostics. Volatile organic compounds (VOCs) produced by growing S. aureus or S. pneumoniae cultures on blood agar plates were detected within minutes and allowed for the distinction of these two bacteria on a species and even strain level within hours. Furthermore, we obtained a fingerprint of clinical patient samples within minutes of measurement and predominantly observed a separation of samples containing live bacteria compared to samples with no bacterial growth. Further development of this technique may reduce the time required for microbiological diagnosis and should help to improve patient’s tailored treatment.http://www.sciencedirect.com/science/article/pii/S2589004222013529Applied microbiologyBiological sciences toolsDiagnosticsMicrobiology |
| spellingShingle | Alejandro Gómez-Mejia Kim Arnold Julian Bär Kapil Dev Singh Thomas C. Scheier Silvio D. Brugger Annelies S. Zinkernagel Pablo Sinues Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites iScience Applied microbiology Biological sciences tools Diagnostics Microbiology |
| title | Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites |
| title_full | Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites |
| title_fullStr | Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites |
| title_full_unstemmed | Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites |
| title_short | Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites |
| title_sort | rapid detection of staphylococcus aureus and streptococcus pneumoniae by real time analysis of volatile metabolites |
| topic | Applied microbiology Biological sciences tools Diagnostics Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222013529 |
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