In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS)
<p>Abstract</p> <p>Background</p> <p>PCV ORF2 capsid protein was predicted to contribute to the control of replication via an interaction between the Cap and Rep proteins in the nucleoplasm. We previously showed that the nuclear localization signal (NLS) on the capsid p...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2013-01-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://www.virologyj.com/content/10/1/16 |
| _version_ | 1818579620913479680 |
|---|---|
| author | Shuai Jiangbing Zhang Xiaofeng Chen Wujian Li Ke Wu Shan He Yongqiang Fang Weihuan |
| author_facet | Shuai Jiangbing Zhang Xiaofeng Chen Wujian Li Ke Wu Shan He Yongqiang Fang Weihuan |
| author_sort | Shuai Jiangbing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>PCV ORF2 capsid protein was predicted to contribute to the control of replication via an interaction between the Cap and Rep proteins in the nucleoplasm. We previously showed that the nuclear localization signal (NLS) on the capsid protein plays an accessory role in the replication of PCV in vitro. To further evaluate the in vivo characteristics of NLS-chimeric PCV DNA clones, BALB/C mice were inoculated intranasally and intraperitoneally with the DNA clones.</p> <p>Results</p> <p>As expected, no gross lesions were detected during the study of the inoculated animals. The chimeric PCV12-, PCV1-NLS2- and PCV2-NLS1-inoculated animals had significantly fewer and less severe histopathological lesions in lymphoid tissues than the PCV2-inoculated animals (P < 0.05). PCV12 induced a specific antibody response against PCV2 ORF2 comparable to that induced by wild-type PCV2 but demonstrated a shorter period of viremia and much lower level of virus loads in sera than those in PCV2-inoculated mice. Remarkably, the PCV2-NLS1 and PCV1-NLS2 chimeras replicated in inoculated mice and induced specific antibody responses but failed to produce viral antigens in the lymph nodes or a detectable viremia.</p> <p>Conclusions</p> <p>The chimeric PCV2-NLS1 and PCV1-NLS2 demonstrated a lower replication level as compared with wild type of PCV2 or PCV1 in vivo, suggesting that ORF2 NLSs played an accessory role in PCV replication. The chimeric PCV12 is a good candidate for vaccination against PCV2 infection.</p> |
| first_indexed | 2024-12-16T07:04:36Z |
| format | Article |
| id | doaj.art-d51a82059106434f8d6f576b68bc60e6 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-16T07:04:36Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-d51a82059106434f8d6f576b68bc60e62022-12-21T22:40:05ZengBMCVirology Journal1743-422X2013-01-011011610.1186/1743-422X-10-16In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS)Shuai JiangbingZhang XiaofengChen WujianLi KeWu ShanHe YongqiangFang Weihuan<p>Abstract</p> <p>Background</p> <p>PCV ORF2 capsid protein was predicted to contribute to the control of replication via an interaction between the Cap and Rep proteins in the nucleoplasm. We previously showed that the nuclear localization signal (NLS) on the capsid protein plays an accessory role in the replication of PCV in vitro. To further evaluate the in vivo characteristics of NLS-chimeric PCV DNA clones, BALB/C mice were inoculated intranasally and intraperitoneally with the DNA clones.</p> <p>Results</p> <p>As expected, no gross lesions were detected during the study of the inoculated animals. The chimeric PCV12-, PCV1-NLS2- and PCV2-NLS1-inoculated animals had significantly fewer and less severe histopathological lesions in lymphoid tissues than the PCV2-inoculated animals (P < 0.05). PCV12 induced a specific antibody response against PCV2 ORF2 comparable to that induced by wild-type PCV2 but demonstrated a shorter period of viremia and much lower level of virus loads in sera than those in PCV2-inoculated mice. Remarkably, the PCV2-NLS1 and PCV1-NLS2 chimeras replicated in inoculated mice and induced specific antibody responses but failed to produce viral antigens in the lymph nodes or a detectable viremia.</p> <p>Conclusions</p> <p>The chimeric PCV2-NLS1 and PCV1-NLS2 demonstrated a lower replication level as compared with wild type of PCV2 or PCV1 in vivo, suggesting that ORF2 NLSs played an accessory role in PCV replication. The chimeric PCV12 is a good candidate for vaccination against PCV2 infection.</p>http://www.virologyj.com/content/10/1/16Porcine circovirusNuclear localization signalChimericReplication |
| spellingShingle | Shuai Jiangbing Zhang Xiaofeng Chen Wujian Li Ke Wu Shan He Yongqiang Fang Weihuan In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) Virology Journal Porcine circovirus Nuclear localization signal Chimeric Replication |
| title | In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) |
| title_full | In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) |
| title_fullStr | In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) |
| title_full_unstemmed | In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) |
| title_short | In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS) |
| title_sort | in vivo characterization of chimeric pcv dna clones containing heterogeneous capsid protein nuclear localization signals nls |
| topic | Porcine circovirus Nuclear localization signal Chimeric Replication |
| url | http://www.virologyj.com/content/10/1/16 |
| work_keys_str_mv | AT shuaijiangbing invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT zhangxiaofeng invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT chenwujian invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT like invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT wushan invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT heyongqiang invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls AT fangweihuan invivocharacterizationofchimericpcvdnaclonescontainingheterogeneouscapsidproteinnuclearlocalizationsignalsnls |