Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats
<p>Abstract</p> <p>This study was conducted to investigate the effect of different dietary ratios of <sup>13</sup> C to <sup>12</sup> C or <sup>15</sup> N to <sup>14</sup> N on their relative incorporation into tissues. Eighty male ra...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | http://www.jasbsci.com/content/3/1/14 |
| _version_ | 1811296971956158464 |
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| author | Lv Wentao Ju Tingting Dong Bing Yu Boyang Yin Jingdong |
| author_facet | Lv Wentao Ju Tingting Dong Bing Yu Boyang Yin Jingdong |
| author_sort | Lv Wentao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>This study was conducted to investigate the effect of different dietary ratios of <sup>13</sup> C to <sup>12</sup> C or <sup>15</sup> N to <sup>14</sup> N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ<sup>13</sup>C levels (δ<sup>13</sup>C = −13.89‰ and δ<sup>15</sup>N = 2.37‰ in experiment 1 and δ<sup>13</sup>C = −19.34‰ and δ<sup>15</sup>N = 4.73‰ in experiment 2) or low δ<sup>13</sup>C levels (δ<sup>13</sup>C = −17.90‰ and δ<sup>15</sup>N = 3.08‰ in experiment 1 and δ<sup>13</sup>C = −21.76‰ and δ<sup>15</sup>N = 0.53‰ in experiment 2), meanwhile, the dietary δ<sup>15</sup>N levels were designed to two ranks. Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of <sup>13</sup> C, <sup>12</sup> C, <sup>15</sup> N and <sup>14</sup> N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete <sup>13</sup> C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary <sup>13</sup> C levels decreased tissue δ<sup>13</sup>C values whereas high dietary <sup>13</sup> C levels did not alter tissue δ<sup>13</sup>C values during the 21-d experiment. Blood δ<sup>15</sup>N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary isotopic signatures and their incorporation rates into rat tissues. However, more studies are needed to illustrate the mechanism through which dietary isotopic ratios influence the extent of isotopic incorporation into the tissues.</p> |
| first_indexed | 2024-04-13T05:57:11Z |
| format | Article |
| id | doaj.art-d51b586c5d4e4a119ccda5b3ee38ed37 |
| institution | Directory Open Access Journal |
| issn | 1674-9782 2049-1891 |
| language | English |
| last_indexed | 2024-04-13T05:57:11Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-d51b586c5d4e4a119ccda5b3ee38ed372022-12-22T02:59:36ZengBMCJournal of Animal Science and Biotechnology1674-97822049-18912012-05-01311410.1186/2049-1891-3-14Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of ratsLv WentaoJu TingtingDong BingYu BoyangYin Jingdong<p>Abstract</p> <p>This study was conducted to investigate the effect of different dietary ratios of <sup>13</sup> C to <sup>12</sup> C or <sup>15</sup> N to <sup>14</sup> N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ<sup>13</sup>C levels (δ<sup>13</sup>C = −13.89‰ and δ<sup>15</sup>N = 2.37‰ in experiment 1 and δ<sup>13</sup>C = −19.34‰ and δ<sup>15</sup>N = 4.73‰ in experiment 2) or low δ<sup>13</sup>C levels (δ<sup>13</sup>C = −17.90‰ and δ<sup>15</sup>N = 3.08‰ in experiment 1 and δ<sup>13</sup>C = −21.76‰ and δ<sup>15</sup>N = 0.53‰ in experiment 2), meanwhile, the dietary δ<sup>15</sup>N levels were designed to two ranks. Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of <sup>13</sup> C, <sup>12</sup> C, <sup>15</sup> N and <sup>14</sup> N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete <sup>13</sup> C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary <sup>13</sup> C levels decreased tissue δ<sup>13</sup>C values whereas high dietary <sup>13</sup> C levels did not alter tissue δ<sup>13</sup>C values during the 21-d experiment. Blood δ<sup>15</sup>N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary isotopic signatures and their incorporation rates into rat tissues. However, more studies are needed to illustrate the mechanism through which dietary isotopic ratios influence the extent of isotopic incorporation into the tissues.</p>http://www.jasbsci.com/content/3/1/14CarbonDietNitrogenStable isotopes |
| spellingShingle | Lv Wentao Ju Tingting Dong Bing Yu Boyang Yin Jingdong Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats Journal of Animal Science and Biotechnology Carbon Diet Nitrogen Stable isotopes |
| title | Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| title_full | Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| title_fullStr | Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| title_full_unstemmed | Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| title_short | Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| title_sort | effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats |
| topic | Carbon Diet Nitrogen Stable isotopes |
| url | http://www.jasbsci.com/content/3/1/14 |
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