Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration
Many dairy operations uses a Brix refractometer to assess the quality of first-milking colostrum. This study investigated whether a digital Brix refractometer could be used in a model to predict colostrum IgG concentration and whether more than one %Brix threshold could be used for different colostr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1240227/full |
| _version_ | 1797674093426769920 |
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| author | Donald Sockett Ryan M. Breuer Ryan M. Breuer Lindsey W. Smith Nicholas S. Keuler Thomas Earleywine |
| author_facet | Donald Sockett Ryan M. Breuer Ryan M. Breuer Lindsey W. Smith Nicholas S. Keuler Thomas Earleywine |
| author_sort | Donald Sockett |
| collection | DOAJ |
| description | Many dairy operations uses a Brix refractometer to assess the quality of first-milking colostrum. This study investigated whether a digital Brix refractometer could be used in a model to predict colostrum IgG concentration and whether more than one %Brix threshold could be used for different colostrum IgG concentrations. Colostrum from 182 animals was tested using a digital Brix refractometer and by single radial immunodiffusion. Statistical analysis, using simple linear regression to relate %Brix results with corresponding colostral IgG concentration, and receiver operating characteristic (ROC) analysis were used to identify %Brix cutoffs that had no false positive results. Colostral IgG concentrations from digital Brix refractometry had a R2 value of 0.818 and a S-value of 21.7 g/L. The large S-value shows that a digital Brix refractometer should not be used in a model to predict colostrum IgG concentration. However, %Brix scores of 19.0, 22.0, 25.0 and 30.0 percent can be used to estimate minimum colostral IgG concentrations of 25, 50, 75 and 100 g/L. These four cutoffs can be used to strategically feed smaller volumes of colostrum to newborn calves. Smaller volumes may reduce unwanted side effects and shorten the time interval in which calves refuse to nurse, while still delivering an adequate mass of IgG to have successful transfer of passive immunity. |
| first_indexed | 2024-03-11T21:54:09Z |
| format | Article |
| id | doaj.art-d51dd4c30a9647e2a469f297c44b30d0 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T21:54:09Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-d51dd4c30a9647e2a469f297c44b30d02023-09-26T05:34:19ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-09-011010.3389/fvets.2023.12402271240227Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentrationDonald Sockett0Ryan M. Breuer1Ryan M. Breuer2Lindsey W. Smith3Nicholas S. Keuler4Thomas Earleywine5Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI, United StatesWisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United StatesWisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Statistics, University of Wisconsin-Madison, Madison, WI, United StatesLand O'Lakes, Cottage Grove, WI, United StatesMany dairy operations uses a Brix refractometer to assess the quality of first-milking colostrum. This study investigated whether a digital Brix refractometer could be used in a model to predict colostrum IgG concentration and whether more than one %Brix threshold could be used for different colostrum IgG concentrations. Colostrum from 182 animals was tested using a digital Brix refractometer and by single radial immunodiffusion. Statistical analysis, using simple linear regression to relate %Brix results with corresponding colostral IgG concentration, and receiver operating characteristic (ROC) analysis were used to identify %Brix cutoffs that had no false positive results. Colostral IgG concentrations from digital Brix refractometry had a R2 value of 0.818 and a S-value of 21.7 g/L. The large S-value shows that a digital Brix refractometer should not be used in a model to predict colostrum IgG concentration. However, %Brix scores of 19.0, 22.0, 25.0 and 30.0 percent can be used to estimate minimum colostral IgG concentrations of 25, 50, 75 and 100 g/L. These four cutoffs can be used to strategically feed smaller volumes of colostrum to newborn calves. Smaller volumes may reduce unwanted side effects and shorten the time interval in which calves refuse to nurse, while still delivering an adequate mass of IgG to have successful transfer of passive immunity.https://www.frontiersin.org/articles/10.3389/fvets.2023.1240227/fullcolostrumbrix refractometryimmunoglobulin Gtransfer of passive immunityradial immunodiffusion |
| spellingShingle | Donald Sockett Ryan M. Breuer Ryan M. Breuer Lindsey W. Smith Nicholas S. Keuler Thomas Earleywine Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration Frontiers in Veterinary Science colostrum brix refractometry immunoglobulin G transfer of passive immunity radial immunodiffusion |
| title | Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration |
| title_full | Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration |
| title_fullStr | Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration |
| title_full_unstemmed | Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration |
| title_short | Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration |
| title_sort | investigation of brix refractometry for estimating bovine colostrum immunoglobulin g concentration |
| topic | colostrum brix refractometry immunoglobulin G transfer of passive immunity radial immunodiffusion |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1240227/full |
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