CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation
The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121003378 |
| _version_ | 1828918815091589120 |
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| author | Janik Boehnke Salim Atakhanov Marcelo A.S. Toledo Herdit M. Schüler Stephanie Sontag Nicolas Chatain Steffen Koschmieder Tim H. Brümmendorf Rafael Kramann Martin Zenke |
| author_facet | Janik Boehnke Salim Atakhanov Marcelo A.S. Toledo Herdit M. Schüler Stephanie Sontag Nicolas Chatain Steffen Koschmieder Tim H. Brümmendorf Rafael Kramann Martin Zenke |
| author_sort | Janik Boehnke |
| collection | DOAJ |
| description | The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4KO iPS cells showed deletion of exon 1 and complete loss of CXCL4 protein. Pluripotency of iPS cells was confirmed by expression of pluripotency markers and trilineage differentiation. CXCL4KO iPS cells are expected to provide a valuable tool for investigating the role of CXCL4 in human diseases. |
| first_indexed | 2024-12-13T21:17:06Z |
| format | Article |
| id | doaj.art-d5201e98c76548fb80312315e5bbd47d |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-13T21:17:06Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-d5201e98c76548fb80312315e5bbd47d2022-12-21T23:31:13ZengElsevierStem Cell Research1873-50612021-08-0155102490CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutationJanik Boehnke0Salim Atakhanov1Marcelo A.S. Toledo2Herdit M. Schüler3Stephanie Sontag4Nicolas Chatain5Steffen Koschmieder6Tim H. Brümmendorf7Rafael Kramann8Martin Zenke9Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, GermanyInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, GermanyInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany; Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, RWTH Aachen University Hospital, Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), Aachen, GermanyInstitute for Human Genetics, RWTH Aachen University Hospital, Aachen, GermanyInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, GermanyDepartment of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, RWTH Aachen University Hospital, Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), Aachen, GermanyDepartment of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, RWTH Aachen University Hospital, Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), Aachen, GermanyDepartment of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, RWTH Aachen University Hospital, Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), Aachen, GermanyInstitute of Experimental Medicine and Systems Biology, RWTH Aachen University Medical School, Aachen, GermanyInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany; Corresponding author at: Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Hospital, Pauwelsstrasse 30, Aachen 52074, Germany.The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4KO iPS cells showed deletion of exon 1 and complete loss of CXCL4 protein. Pluripotency of iPS cells was confirmed by expression of pluripotency markers and trilineage differentiation. CXCL4KO iPS cells are expected to provide a valuable tool for investigating the role of CXCL4 in human diseases.http://www.sciencedirect.com/science/article/pii/S1873506121003378Induced pluripotent stem celliPS cellsHematopoiesisCXCL4PF4JAK2 V617F |
| spellingShingle | Janik Boehnke Salim Atakhanov Marcelo A.S. Toledo Herdit M. Schüler Stephanie Sontag Nicolas Chatain Steffen Koschmieder Tim H. Brümmendorf Rafael Kramann Martin Zenke CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation Stem Cell Research Induced pluripotent stem cell iPS cells Hematopoiesis CXCL4 PF4 JAK2 V617F |
| title | CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation |
| title_full | CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation |
| title_fullStr | CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation |
| title_full_unstemmed | CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation |
| title_short | CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation |
| title_sort | crispr cas9 mediated cxcl4 knockout in human ips cells of polycythemia vera patient with jak2 v617f mutation |
| topic | Induced pluripotent stem cell iPS cells Hematopoiesis CXCL4 PF4 JAK2 V617F |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121003378 |
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