IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES
Objective: To evaluate the diagnostic value of Immunohistochemistry (IHC) for finding BRAFV600E mutation in melanoma patients. Study Design: Cross-sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, Jan 2018 to A...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Army Medical College Rawalpindi
2021-10-01
|
| Series: | Pakistan Armed Forces Medical Journal |
| Subjects: | |
| Online Access: | https://www.pafmj.org/index.php/PAFMJ/article/view/5955/3643 |
| _version_ | 1818435017773154304 |
|---|---|
| author | Muhammad Umer Ch Farhan Akhtar Nighat Jamal Rabia Ahmad Sumaira Bukhsh Hamza Mansur |
| author_facet | Muhammad Umer Ch Farhan Akhtar Nighat Jamal Rabia Ahmad Sumaira Bukhsh Hamza Mansur |
| author_sort | Muhammad Umer Ch |
| collection | DOAJ |
| description | Objective: To evaluate the diagnostic value of Immunohistochemistry (IHC) for finding BRAFV600E mutation in melanoma patients.
Study Design: Cross-sectional study.
Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, Jan 2018 to Apr 2019.
Methodology: Hundred histologically confirmed cases of melanoma aged between 18-80 years were included over a period of one year. To create the tissue microarray and to conduct immunohistochemistry with VE1 antibody melanoma lesion samples were selected.
Results: Ninety-four cases out of 100, were melanoma positive for BRAFV600E detected on immunohistochemistry (IHC) using a monoclonal antibody. The skin was the primary site in seventy-nine (79%) patients, unknown (metastatic) in nineteen and uveal was only in two cases. The diagnostic significance of immunohistochemistry (IHC) for detecting the BRAFV600E mutation in melanoma patients was independent of age, site and stage at diagnosis (p-value <0.05).
Conclusion: Testing of BRAV600E mutation by immunohistochemistry is a rapid, reliable and cost-effective tool in melanoma patients which may further help clinicians in initiating targeted therapy for these patients. |
| first_indexed | 2024-12-14T16:46:12Z |
| format | Article |
| id | doaj.art-d5205509b351480e8f9352c9379000b6 |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-12-14T16:46:12Z |
| publishDate | 2021-10-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-d5205509b351480e8f9352c9379000b62022-12-21T22:54:11ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422021-10-0171518851888IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASESMuhammad Umer Ch0Farhan Akhtar1Nighat Jamal2Rabia Ahmad3Sumaira Bukhsh4Hamza Mansur5Pakistan Naval Ship Shifa Hospital, Karachi PakistanPak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanPakistan Naval Ship Shifa Hospital, Karachi PakistanPakistan Naval Ship Shifa Hospital, Karachi PakistanCombined Military Hospital Malir/National University of Medical Sciences (NUMS) PakistanArmed Forces Institute of Pathology/ National University of Medical Sciences (NUMS) Rawalpindi PakistanObjective: To evaluate the diagnostic value of Immunohistochemistry (IHC) for finding BRAFV600E mutation in melanoma patients. Study Design: Cross-sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, Jan 2018 to Apr 2019. Methodology: Hundred histologically confirmed cases of melanoma aged between 18-80 years were included over a period of one year. To create the tissue microarray and to conduct immunohistochemistry with VE1 antibody melanoma lesion samples were selected. Results: Ninety-four cases out of 100, were melanoma positive for BRAFV600E detected on immunohistochemistry (IHC) using a monoclonal antibody. The skin was the primary site in seventy-nine (79%) patients, unknown (metastatic) in nineteen and uveal was only in two cases. The diagnostic significance of immunohistochemistry (IHC) for detecting the BRAFV600E mutation in melanoma patients was independent of age, site and stage at diagnosis (p-value <0.05). Conclusion: Testing of BRAV600E mutation by immunohistochemistry is a rapid, reliable and cost-effective tool in melanoma patients which may further help clinicians in initiating targeted therapy for these patients.https://www.pafmj.org/index.php/PAFMJ/article/view/5955/3643brafv600e mutationimmunohistochemistrymonoclonal antibody |
| spellingShingle | Muhammad Umer Ch Farhan Akhtar Nighat Jamal Rabia Ahmad Sumaira Bukhsh Hamza Mansur IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES Pakistan Armed Forces Medical Journal brafv600e mutation immunohistochemistry monoclonal antibody |
| title | IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES |
| title_full | IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES |
| title_fullStr | IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES |
| title_full_unstemmed | IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES |
| title_short | IMMUNOHISTOCHEMICAL EXPRESSION OF BRAFV600E IN MELANOMA CASES |
| title_sort | immunohistochemical expression of brafv600e in melanoma cases |
| topic | brafv600e mutation immunohistochemistry monoclonal antibody |
| url | https://www.pafmj.org/index.php/PAFMJ/article/view/5955/3643 |
| work_keys_str_mv | AT muhammadumerch immunohistochemicalexpressionofbrafv600einmelanomacases AT farhanakhtar immunohistochemicalexpressionofbrafv600einmelanomacases AT nighatjamal immunohistochemicalexpressionofbrafv600einmelanomacases AT rabiaahmad immunohistochemicalexpressionofbrafv600einmelanomacases AT sumairabukhsh immunohistochemicalexpressionofbrafv600einmelanomacases AT hamzamansur immunohistochemicalexpressionofbrafv600einmelanomacases |