KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells
Previous studies have strongly recommended that KISS-1 metastasis suppressor (<i>KISS1</i>) plays an essential gatekeeper of the initiation of reproductive maturation in mammals. However, <i>KISS1</i> has been recently reported to highly express in ovarian granulosa cells (GC...
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MDPI AG
2019-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/2/54 |
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| author | Xiaoping Xin Zhonghui Li Yuyi Zhong Qingqing Li Jiaying Wang Hao Zhang Xiaolong Yuan Jiaqi Li Zhe Zhang |
| author_facet | Xiaoping Xin Zhonghui Li Yuyi Zhong Qingqing Li Jiaying Wang Hao Zhang Xiaolong Yuan Jiaqi Li Zhe Zhang |
| author_sort | Xiaoping Xin |
| collection | DOAJ |
| description | Previous studies have strongly recommended that KISS-1 metastasis suppressor (<i>KISS1</i>) plays an essential gatekeeper of the initiation of reproductive maturation in mammals. However, <i>KISS1</i> has been recently reported to highly express in ovarian granulosa cells (GCs). But the biological functionalities of <i>KISS1</i> on cell apoptosis, cell cycle, and synthesis of estradiol-17β (E2) have not been explored in GCs. In this study, using porcine GCs as a cellular model, the overexpression plasmid of <i>KISS1</i> was built to explore the biological effects of <i>KISS1</i> on the PI3K signaling pathway, estrogen signaling pathway, cell apoptosis, cell cycle, and E2 secretion. We found that mRNA of <i>KISS1</i> highly expressed in the ovary and significantly increased from immature to mature follicles in gilts. Overexpression of <i>KISS1</i> could significantly increase the mRNA expression of <i>PIK3CG, PIK3C1,</i> and <i>PDK1</i>, and significantly decreased the mRNA levels of <i>FOXO3</i>, <i>TSC2</i>, and <i>BAD</i> of PI3K signaling pathway. Furthermore, results of the flow cytometry showed that overexpression of <i>KISS1</i> significantly inhibited the apoptosis of GCs and decreased the percentage of GCs at G0/G1 phase of the cell cycle. Additionally, overexpression of <i>KISS1</i> could increase the mRNA levels of <i>Star</i>, <i>CYP17</i>, <i>3B-HSD</i>, <i>17B-HSD</i> of estrogen synthesis signaling pathway, significantly increase the concentration of E2 in the supernatant of the cultured GCs, and up-regulate the mRNA expression levels of <i>ESR1</i> and <i>ESR2</i>. These results suggested that <i>KISS1</i> might suppress cell apoptosis through activating the PI3K signaling pathway and stimulate synthesis of E2 via boosting the estrogen synthesis signaling pathway. This study would be of great interests for exploring the biological functionalities of <i>KISS1</i> in the folliculogenesis and sex steroid production of the ovaries in mammals. |
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| spelling | doaj.art-d520e4c5d19649c6be52d65f44a9ac172022-12-22T00:45:38ZengMDPI AGAnimals2076-26152019-02-01925410.3390/ani9020054ani9020054KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa CellsXiaoping Xin0Zhonghui Li1Yuyi Zhong2Qingqing Li3Jiaying Wang4Hao Zhang5Xiaolong Yuan6Jiaqi Li7Zhe Zhang8Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaPrevious studies have strongly recommended that KISS-1 metastasis suppressor (<i>KISS1</i>) plays an essential gatekeeper of the initiation of reproductive maturation in mammals. However, <i>KISS1</i> has been recently reported to highly express in ovarian granulosa cells (GCs). But the biological functionalities of <i>KISS1</i> on cell apoptosis, cell cycle, and synthesis of estradiol-17β (E2) have not been explored in GCs. In this study, using porcine GCs as a cellular model, the overexpression plasmid of <i>KISS1</i> was built to explore the biological effects of <i>KISS1</i> on the PI3K signaling pathway, estrogen signaling pathway, cell apoptosis, cell cycle, and E2 secretion. We found that mRNA of <i>KISS1</i> highly expressed in the ovary and significantly increased from immature to mature follicles in gilts. Overexpression of <i>KISS1</i> could significantly increase the mRNA expression of <i>PIK3CG, PIK3C1,</i> and <i>PDK1</i>, and significantly decreased the mRNA levels of <i>FOXO3</i>, <i>TSC2</i>, and <i>BAD</i> of PI3K signaling pathway. Furthermore, results of the flow cytometry showed that overexpression of <i>KISS1</i> significantly inhibited the apoptosis of GCs and decreased the percentage of GCs at G0/G1 phase of the cell cycle. Additionally, overexpression of <i>KISS1</i> could increase the mRNA levels of <i>Star</i>, <i>CYP17</i>, <i>3B-HSD</i>, <i>17B-HSD</i> of estrogen synthesis signaling pathway, significantly increase the concentration of E2 in the supernatant of the cultured GCs, and up-regulate the mRNA expression levels of <i>ESR1</i> and <i>ESR2</i>. These results suggested that <i>KISS1</i> might suppress cell apoptosis through activating the PI3K signaling pathway and stimulate synthesis of E2 via boosting the estrogen synthesis signaling pathway. This study would be of great interests for exploring the biological functionalities of <i>KISS1</i> in the folliculogenesis and sex steroid production of the ovaries in mammals.https://www.mdpi.com/2076-2615/9/2/54<i>KISS1</i>Granulosa cellsCell apoptosisCell cycleSynthesis of E2 |
| spellingShingle | Xiaoping Xin Zhonghui Li Yuyi Zhong Qingqing Li Jiaying Wang Hao Zhang Xiaolong Yuan Jiaqi Li Zhe Zhang KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells Animals <i>KISS1</i> Granulosa cells Cell apoptosis Cell cycle Synthesis of E2 |
| title | KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells |
| title_full | KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells |
| title_fullStr | KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells |
| title_full_unstemmed | KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells |
| title_short | KISS1 Suppresses Apoptosis and Stimulates the Synthesis of E2 in Porcine Ovarian Granulosa Cells |
| title_sort | kiss1 suppresses apoptosis and stimulates the synthesis of e2 in porcine ovarian granulosa cells |
| topic | <i>KISS1</i> Granulosa cells Cell apoptosis Cell cycle Synthesis of E2 |
| url | https://www.mdpi.com/2076-2615/9/2/54 |
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