New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater
Antibiotic resistance of the indicator microorganism <i>Escherichia coli</i> was investigated in isolates from samples collected during the course of one year from two wastewater treatment plants treating municipal and animal wastes in Slovakia, respectively. The genes of antibiotic resi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/9/1111 |
| _version_ | 1797520491708153856 |
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| author | Gabriela Gregova Vladimir Kmet Tatiana Szaboova |
| author_facet | Gabriela Gregova Vladimir Kmet Tatiana Szaboova |
| author_sort | Gabriela Gregova |
| collection | DOAJ |
| description | Antibiotic resistance of the indicator microorganism <i>Escherichia coli</i> was investigated in isolates from samples collected during the course of one year from two wastewater treatment plants treating municipal and animal wastes in Slovakia, respectively. The genes of antibiotic resistance and virulence factors in selected resistant <i>E. coli</i> isolates were described. A high percentage of the isolates from municipal and animal wastewater were resistant to ampicillin, streptomycin, tetracycline, ceftiofur, ceftriaxone, and enrofloxacin. In the selected <i>E. coli</i> isolates, we detected the following phenotypes: ESBL (20.4% in animal wastewater; 7.7% in municipal wastewater), multidrug-resistant (17% of animal and 32% of municipal isolates), high resistance to quinolones (25% of animal and 48% of municipal samples), and CTX-M (7.9% of animal and 17.3% of municipal isolates). We confirmed an integro-mediated antibiotic resistance in 13 <i>E. coli</i> strains from municipal and animal wastewater samples, of which the <i>Tn3</i> gene and virulence genes <i>cvaC</i>, <i>iutA</i>, <i>iss</i>, <i>ibeA</i>, <i>kps</i>, and <i>papC</i> were detected in six isolates. One of the strains of pathogenic <i>E. coli</i> from the animal wastewater contained genes <i>ibeA</i> with <i>papC</i>, <i>iss</i>, <i>kpsII</i>, <i>Int1</i>, <i>Tn3</i>, and <i>Cit.</i> In addition, one bla<sub>IMP</sub> gene was found in the municipal wastewater sample. This emphasises the importance of using the appropriate treatment methods to reduce the counts of antibiotic-resistant microorganisms in wastewater effluent. |
| first_indexed | 2024-03-10T07:57:30Z |
| format | Article |
| id | doaj.art-d520e5891bbb43b09890c04e564f2dd3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T07:57:30Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d520e5891bbb43b09890c04e564f2dd32023-11-22T11:46:26ZengMDPI AGAntibiotics2079-63822021-09-01109111110.3390/antibiotics10091111New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal WastewaterGabriela Gregova0Vladimir Kmet1Tatiana Szaboova2The University of Veterinary Medicine and Pharmacy in Košice, Komenského 87, 040 01 Košice, SlovakiaCentre of Biosciences, Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4, 040 01 Košice, SlovakiaThe University of Veterinary Medicine and Pharmacy in Košice, Komenského 87, 040 01 Košice, SlovakiaAntibiotic resistance of the indicator microorganism <i>Escherichia coli</i> was investigated in isolates from samples collected during the course of one year from two wastewater treatment plants treating municipal and animal wastes in Slovakia, respectively. The genes of antibiotic resistance and virulence factors in selected resistant <i>E. coli</i> isolates were described. A high percentage of the isolates from municipal and animal wastewater were resistant to ampicillin, streptomycin, tetracycline, ceftiofur, ceftriaxone, and enrofloxacin. In the selected <i>E. coli</i> isolates, we detected the following phenotypes: ESBL (20.4% in animal wastewater; 7.7% in municipal wastewater), multidrug-resistant (17% of animal and 32% of municipal isolates), high resistance to quinolones (25% of animal and 48% of municipal samples), and CTX-M (7.9% of animal and 17.3% of municipal isolates). We confirmed an integro-mediated antibiotic resistance in 13 <i>E. coli</i> strains from municipal and animal wastewater samples, of which the <i>Tn3</i> gene and virulence genes <i>cvaC</i>, <i>iutA</i>, <i>iss</i>, <i>ibeA</i>, <i>kps</i>, and <i>papC</i> were detected in six isolates. One of the strains of pathogenic <i>E. coli</i> from the animal wastewater contained genes <i>ibeA</i> with <i>papC</i>, <i>iss</i>, <i>kpsII</i>, <i>Int1</i>, <i>Tn3</i>, and <i>Cit.</i> In addition, one bla<sub>IMP</sub> gene was found in the municipal wastewater sample. This emphasises the importance of using the appropriate treatment methods to reduce the counts of antibiotic-resistant microorganisms in wastewater effluent.https://www.mdpi.com/2079-6382/10/9/1111<i>E. coli</i>animal wastewatermunicipal wastewaterresistanceESBL |
| spellingShingle | Gabriela Gregova Vladimir Kmet Tatiana Szaboova New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater Antibiotics <i>E. coli</i> animal wastewater municipal wastewater resistance ESBL |
| title | New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater |
| title_full | New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater |
| title_fullStr | New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater |
| title_full_unstemmed | New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater |
| title_short | New Insight on Antibiotic Resistance and Virulence of <i>Escherichia coli</i> from Municipal and Animal Wastewater |
| title_sort | new insight on antibiotic resistance and virulence of i escherichia coli i from municipal and animal wastewater |
| topic | <i>E. coli</i> animal wastewater municipal wastewater resistance ESBL |
| url | https://www.mdpi.com/2079-6382/10/9/1111 |
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