Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model
Long term high-fat diet (HFD) will lead to obesity and its complications, such as lipid metabolism disorder, insulin resistance and inflammation. The purpose of this study is to demonstrate the mechanism of Lactobacillus paracasei Jlus66 to improve dietary fatty liver in obese mice model. C57BL/6J m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002201 |
| _version_ | 1818502311610155008 |
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| author | Yaoyao Ji Qiang Xie Xiangren Meng Wei Wang Shengnan Li Xinsong Lang Changhui Zhao Yuan Yuan Haiqing Ye |
| author_facet | Yaoyao Ji Qiang Xie Xiangren Meng Wei Wang Shengnan Li Xinsong Lang Changhui Zhao Yuan Yuan Haiqing Ye |
| author_sort | Yaoyao Ji |
| collection | DOAJ |
| description | Long term high-fat diet (HFD) will lead to obesity and its complications, such as lipid metabolism disorder, insulin resistance and inflammation. The purpose of this study is to demonstrate the mechanism of Lactobacillus paracasei Jlus66 to improve dietary fatty liver in obese mice model. C57BL/6J male mice were randomly divided into three groups (Control, HFD and HFD + Jlus66). Proteins of many pathways related to insulin resistance (IRS-1, AKT, GSK3β, GLUT4 and PTP1B), to jejunum permeability (Zo-1, Claudin-1 and Occludin) were detected by using western blotting. Correspondingly, genes of inflammation (Inos, Tnf-α and Il-1β), mitochondrial dysfunction (Cox5b, Cox7a1, CoxⅡ, Cox8b, Cycs and Sirt1) and oxidative stress (yGcs, Mnsod, Nqo-1, Gpx1 and Ho-1) were detected by using RT-qPCR. As results, Jlus66 effectively improved lipid metabolism disorder and mitochondrial dysfunction, ameliorated insulin resistance and inflammatory response in the liver, jejunum and adipose tissue of HFD-induced obese mice model. Moreover, Jlus66 treated also protected jejunum barrier integrity and alleviated oxidative stress. In conclusion, Jlus66 might be a nutritional strategy for obesity and its complications. |
| first_indexed | 2024-12-10T21:08:33Z |
| format | Article |
| id | doaj.art-d522d0cd15314842a497c9e705d5ab2b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-10T21:08:33Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d522d0cd15314842a497c9e705d5ab2b2022-12-22T01:33:33ZengElsevierJournal of Functional Foods1756-46462022-08-0195105150Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice modelYaoyao Ji0Qiang Xie1Xiangren Meng2Wei Wang3Shengnan Li4Xinsong Lang5Changhui Zhao6Yuan Yuan7Haiqing Ye8College of Food Science and Engineering, Jilin University, Changchun, ChinaWuxi Vocational Institute of Commerce, Wu Xi, ChinaFood Science and Engineering, Yangzhou University, Yangzhou, ChinaJilin Provincial People's Hospital, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, ChinaCollege of Food Science and Engineering, Jilin University, Changchun, China; Corresponding author.Long term high-fat diet (HFD) will lead to obesity and its complications, such as lipid metabolism disorder, insulin resistance and inflammation. The purpose of this study is to demonstrate the mechanism of Lactobacillus paracasei Jlus66 to improve dietary fatty liver in obese mice model. C57BL/6J male mice were randomly divided into three groups (Control, HFD and HFD + Jlus66). Proteins of many pathways related to insulin resistance (IRS-1, AKT, GSK3β, GLUT4 and PTP1B), to jejunum permeability (Zo-1, Claudin-1 and Occludin) were detected by using western blotting. Correspondingly, genes of inflammation (Inos, Tnf-α and Il-1β), mitochondrial dysfunction (Cox5b, Cox7a1, CoxⅡ, Cox8b, Cycs and Sirt1) and oxidative stress (yGcs, Mnsod, Nqo-1, Gpx1 and Ho-1) were detected by using RT-qPCR. As results, Jlus66 effectively improved lipid metabolism disorder and mitochondrial dysfunction, ameliorated insulin resistance and inflammatory response in the liver, jejunum and adipose tissue of HFD-induced obese mice model. Moreover, Jlus66 treated also protected jejunum barrier integrity and alleviated oxidative stress. In conclusion, Jlus66 might be a nutritional strategy for obesity and its complications.http://www.sciencedirect.com/science/article/pii/S1756464622002201High fat dietFatty liverInsulin resistanceInflammation respondsMetabolic disorder |
| spellingShingle | Yaoyao Ji Qiang Xie Xiangren Meng Wei Wang Shengnan Li Xinsong Lang Changhui Zhao Yuan Yuan Haiqing Ye Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model Journal of Functional Foods High fat diet Fatty liver Insulin resistance Inflammation responds Metabolic disorder |
| title | Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| title_full | Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| title_fullStr | Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| title_full_unstemmed | Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| title_short | Lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| title_sort | lactobacillus paracasei improves dietary fatty liver by reducing insulin resistance and inflammation in obese mice model |
| topic | High fat diet Fatty liver Insulin resistance Inflammation responds Metabolic disorder |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002201 |
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