Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification
The utilization of herbal extraction residues (HERs) for production of valuable products has gained significant momentum in recent years. In this study, Lianhua Qingwen residue (LQR) was selected as a representative HER for enzymatic saccharification. By subjecting starch-containing raw unpretreated...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Results in Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590123023006576 |
| _version_ | 1797385178113376256 |
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| author | Zhenzhou Zhu Sirong Wu Benkun Qi Caixia Wang Jianquan Luo Yinhua Wan |
| author_facet | Zhenzhou Zhu Sirong Wu Benkun Qi Caixia Wang Jianquan Luo Yinhua Wan |
| author_sort | Zhenzhou Zhu |
| collection | DOAJ |
| description | The utilization of herbal extraction residues (HERs) for production of valuable products has gained significant momentum in recent years. In this study, Lianhua Qingwen residue (LQR) was selected as a representative HER for enzymatic saccharification. By subjecting starch-containing raw unpretreated LQR to direct hydrolysis using a combination of cellulolytic and amylolytic enzyme, a glucan conversion of around 60 % was achieved within 48 h. To further enhance the hydrolysis rate and yield, ultrasound treatment of LQR samples was conducted. Investigation into the effect of ultrasound treatment on subsequent enzymatic saccharification revealed a noteworthy acceleration in the saccharification rate of LQR, exceeding 20 % within 10 h of hydrolysis time. Notably, this enhancement was achieved even when the substrate concentration was high at 10 % under optimized conditions of 5 min of sonication at a power intensity of 1.20 kW/cm2 and a temperature of 50 °C. The improvement in efficiency can be attributed to the disruptive effect of ultrasound treatment on the structure of LQR, leading to a reduction in particle size. Consequently, the LQR particles exhibited better contact with the enzyme, promoting a more efficient saccharification process. These findings suggest that ultrasound technology holds considerable potential for enhancing the enzymatic saccharification of HERs. |
| first_indexed | 2024-03-08T21:50:23Z |
| format | Article |
| id | doaj.art-d526697865064fd9b7304a86183e181b |
| institution | Directory Open Access Journal |
| issn | 2590-1230 |
| language | English |
| last_indexed | 2024-03-08T21:50:23Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Engineering |
| spelling | doaj.art-d526697865064fd9b7304a86183e181b2023-12-20T07:36:05ZengElsevierResults in Engineering2590-12302023-12-0120101530Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharificationZhenzhou Zhu0Sirong Wu1Benkun Qi2Caixia Wang3Jianquan Luo4Yinhua Wan5Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, ChinaNational R&D Center for Se-rich Agricultural Products Processing, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; Corresponding author.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaUniversity of Chinese Academy of Sciences, Beijing, 100049, China; Ganjiang Innovation Academy, Chinese Academy of Sciences, Ganzhou, 341119, ChinaThe utilization of herbal extraction residues (HERs) for production of valuable products has gained significant momentum in recent years. In this study, Lianhua Qingwen residue (LQR) was selected as a representative HER for enzymatic saccharification. By subjecting starch-containing raw unpretreated LQR to direct hydrolysis using a combination of cellulolytic and amylolytic enzyme, a glucan conversion of around 60 % was achieved within 48 h. To further enhance the hydrolysis rate and yield, ultrasound treatment of LQR samples was conducted. Investigation into the effect of ultrasound treatment on subsequent enzymatic saccharification revealed a noteworthy acceleration in the saccharification rate of LQR, exceeding 20 % within 10 h of hydrolysis time. Notably, this enhancement was achieved even when the substrate concentration was high at 10 % under optimized conditions of 5 min of sonication at a power intensity of 1.20 kW/cm2 and a temperature of 50 °C. The improvement in efficiency can be attributed to the disruptive effect of ultrasound treatment on the structure of LQR, leading to a reduction in particle size. Consequently, the LQR particles exhibited better contact with the enzyme, promoting a more efficient saccharification process. These findings suggest that ultrasound technology holds considerable potential for enhancing the enzymatic saccharification of HERs.http://www.sciencedirect.com/science/article/pii/S2590123023006576Herbal residueUltrasoundEnzymatic hydrolysisCellulaseAmylolytic enzymesBioconversion |
| spellingShingle | Zhenzhou Zhu Sirong Wu Benkun Qi Caixia Wang Jianquan Luo Yinhua Wan Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification Results in Engineering Herbal residue Ultrasound Enzymatic hydrolysis Cellulase Amylolytic enzymes Bioconversion |
| title | Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| title_full | Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| title_fullStr | Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| title_full_unstemmed | Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| title_short | Ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| title_sort | ultrasound treatment of herbal extraction residue to enhance enzymatic saccharification |
| topic | Herbal residue Ultrasound Enzymatic hydrolysis Cellulase Amylolytic enzymes Bioconversion |
| url | http://www.sciencedirect.com/science/article/pii/S2590123023006576 |
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