Proteomic Methods of Detection and Quantification of Protein Toxins
Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to comp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-02-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/3/99 |
| _version_ | 1811187550863228928 |
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| author | Miloslava Duracova Jana Klimentova Alena Fucikova Jiri Dresler |
| author_facet | Miloslava Duracova Jana Klimentova Alena Fucikova Jiri Dresler |
| author_sort | Miloslava Duracova |
| collection | DOAJ |
| description | Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to compare conventional detection methods of protein toxins such as in vitro bioassays with proteomic methods, including immunoassays and mass spectrometry-based techniques and their combination. Special emphasis is given to toxins falling into a group of selected agents, according to the Centers for Disease Control and Prevention, such as Staphylococcal enterotoxins, Bacillus anthracis toxins, Clostridium botulinum toxins, Clostridium perfringens epsilon toxin, ricin from Ricinus communis, Abrin from Abrus precatorius or control of trade in dual-use items in the European Union, including lesser known protein toxins such as Viscumin from Viscum album. The analysis of protein toxins and monitoring for biological threats, i.e., the deliberate spread of infectious microorganisms or toxins through water, food, or the air, requires rapid and reliable methods for the early identification of these agents. |
| first_indexed | 2024-04-11T14:05:15Z |
| format | Article |
| id | doaj.art-d528811891754bdeb69f60a6671ba0d2 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T14:05:15Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d528811891754bdeb69f60a6671ba0d22022-12-22T04:19:56ZengMDPI AGToxins2072-66512018-02-011039910.3390/toxins10030099toxins10030099Proteomic Methods of Detection and Quantification of Protein ToxinsMiloslava Duracova0Jana Klimentova1Alena Fucikova2Jiri Dresler3Faculty of Military Health Sciences, University of Defense in Brno, Třebešská 1575, CZ-500 01 Hradec Králové, Czech RepublicFaculty of Military Health Sciences, University of Defense in Brno, Třebešská 1575, CZ-500 01 Hradec Králové, Czech RepublicFaculty of Military Health Sciences, University of Defense in Brno, Třebešská 1575, CZ-500 01 Hradec Králové, Czech RepublicMilitary Health Institute, Military Medical Agency, Tychonova 1, CZ-160 00 Prague 6, Czech RepublicBiological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to compare conventional detection methods of protein toxins such as in vitro bioassays with proteomic methods, including immunoassays and mass spectrometry-based techniques and their combination. Special emphasis is given to toxins falling into a group of selected agents, according to the Centers for Disease Control and Prevention, such as Staphylococcal enterotoxins, Bacillus anthracis toxins, Clostridium botulinum toxins, Clostridium perfringens epsilon toxin, ricin from Ricinus communis, Abrin from Abrus precatorius or control of trade in dual-use items in the European Union, including lesser known protein toxins such as Viscumin from Viscum album. The analysis of protein toxins and monitoring for biological threats, i.e., the deliberate spread of infectious microorganisms or toxins through water, food, or the air, requires rapid and reliable methods for the early identification of these agents.http://www.mdpi.com/2072-6651/10/3/99protein toxinsanalytical methodsproteomicbio-terrorism |
| spellingShingle | Miloslava Duracova Jana Klimentova Alena Fucikova Jiri Dresler Proteomic Methods of Detection and Quantification of Protein Toxins Toxins protein toxins analytical methods proteomic bio-terrorism |
| title | Proteomic Methods of Detection and Quantification of Protein Toxins |
| title_full | Proteomic Methods of Detection and Quantification of Protein Toxins |
| title_fullStr | Proteomic Methods of Detection and Quantification of Protein Toxins |
| title_full_unstemmed | Proteomic Methods of Detection and Quantification of Protein Toxins |
| title_short | Proteomic Methods of Detection and Quantification of Protein Toxins |
| title_sort | proteomic methods of detection and quantification of protein toxins |
| topic | protein toxins analytical methods proteomic bio-terrorism |
| url | http://www.mdpi.com/2072-6651/10/3/99 |
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