Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin
Bovine β-Lactoglobulin (BLG) induces severe cow’s milk allergy. This research investigated the effect of ultrasound-assisted luteolin (LUT) treatment on the structure and allergenicity of BLG. The structure of the treated BLG was characterized by Fourier transform infrared (FTIR) spectroscopy, circ...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-11-007.pdf |
| _version_ | 1797773172873887744 |
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| author | WANG Titi, WANG Yang, ZOU Lin, LIU Jun, SHAO Yanhong, TU Zongcai |
| author_facet | WANG Titi, WANG Yang, ZOU Lin, LIU Jun, SHAO Yanhong, TU Zongcai |
| author_sort | WANG Titi, WANG Yang, ZOU Lin, LIU Jun, SHAO Yanhong, TU Zongcai |
| collection | DOAJ |
| description | Bovine β-Lactoglobulin (BLG) induces severe cow’s milk allergy. This research investigated the effect of ultrasound-assisted luteolin (LUT) treatment on the structure and allergenicity of BLG. The structure of the treated BLG was characterized by Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy and fluorescence spectroscopy, and its allergenicity was evaluated by enzyme-linked immunosorbent assay (ELISA) and cell experiments. It was found that the major interaction force between BLG and LUT was hydrophobic interaction both before and after ultrasonic treatment. Ultrasound-assisted LUT treatment significantly altered the secondary and tertiary structures of BLG, and reduced the IgE-binding ability and the release of interleukin-6, interleukin-4 and histamine from KU812 cells. Therefore, ultrasound-assisted LUT treatment changes the conformational epitope of BLG, reducing its allergenicity, and the best effect can be achieved by secondary ultrasound-assisted LUT treatment. |
| first_indexed | 2024-03-12T22:02:31Z |
| format | Article |
| id | doaj.art-d5298afe96004ce0b191e4ea7b238102 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T22:02:31Z |
| publishDate | 2023-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-d5298afe96004ce0b191e4ea7b2381022023-07-25T06:04:40ZengChina Food Publishing CompanyShipin Kexue1002-66302023-06-014411485610.7506/spkx1002-6630-20220722-261Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-LactoglobulinWANG Titi, WANG Yang, ZOU Lin, LIU Jun, SHAO Yanhong, TU Zongcai0(1. National R&D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)Bovine β-Lactoglobulin (BLG) induces severe cow’s milk allergy. This research investigated the effect of ultrasound-assisted luteolin (LUT) treatment on the structure and allergenicity of BLG. The structure of the treated BLG was characterized by Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy and fluorescence spectroscopy, and its allergenicity was evaluated by enzyme-linked immunosorbent assay (ELISA) and cell experiments. It was found that the major interaction force between BLG and LUT was hydrophobic interaction both before and after ultrasonic treatment. Ultrasound-assisted LUT treatment significantly altered the secondary and tertiary structures of BLG, and reduced the IgE-binding ability and the release of interleukin-6, interleukin-4 and histamine from KU812 cells. Therefore, ultrasound-assisted LUT treatment changes the conformational epitope of BLG, reducing its allergenicity, and the best effect can be achieved by secondary ultrasound-assisted LUT treatment.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-11-007.pdfultrasound; luteolin, β-lactoglobulin; structure; allergenicity |
| spellingShingle | WANG Titi, WANG Yang, ZOU Lin, LIU Jun, SHAO Yanhong, TU Zongcai Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin Shipin Kexue ultrasound; luteolin, β-lactoglobulin; structure; allergenicity |
| title | Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin |
| title_full | Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin |
| title_fullStr | Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin |
| title_full_unstemmed | Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin |
| title_short | Effect of Ultrasound-Assisted Luteolin Treatment on the Structure and Allergenicity of β-Lactoglobulin |
| title_sort | effect of ultrasound assisted luteolin treatment on the structure and allergenicity of β lactoglobulin |
| topic | ultrasound; luteolin, β-lactoglobulin; structure; allergenicity |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-11-007.pdf |
| work_keys_str_mv | AT wangtitiwangyangzoulinliujunshaoyanhongtuzongcai effectofultrasoundassistedluteolintreatmentonthestructureandallergenicityofblactoglobulin |