Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil
The present study aimed to evaluate the safety, antimicrobial and technological properties of lactic acid bacteria from artisanal Colonial-type cheese marketed at streets fair in Pelotas, RS. A total of 73 isolates were characterized as BAL after Gram’s method and catalase test. All strains were gel...
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| Format: | Article |
| Language: | English |
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Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)
2023-04-01
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| Series: | Revista do Instituto de Latícinios Cândido Tostes |
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| Online Access: | https://www.revistadoilct.com.br/rilct/article/view/885 |
| _version_ | 1797839422092214272 |
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| author | Carlos Henrique Gomes de Sousa Lima Nádia Carbonera Elizabete Helbig |
| author_facet | Carlos Henrique Gomes de Sousa Lima Nádia Carbonera Elizabete Helbig |
| author_sort | Carlos Henrique Gomes de Sousa Lima |
| collection | DOAJ |
| description | The present study aimed to evaluate the safety, antimicrobial and technological properties of lactic acid bacteria from artisanal Colonial-type cheese marketed at streets fair in Pelotas, RS. A total of 73 isolates were characterized as BAL after Gram’s method and catalase test. All strains were gelatinase and DNase negative, regarding hemolytic activity, seven were detected as α and two as β-hemolytic, exerting 63 BAL that demonstrated sensitivity to the chloramphenicol and tetracycline antibiotics. The antagonistic activity was verified by the spot-on-the-lawn method and the evaluation of the production of bacteriocins by the diffusion technique in wells. Four BAL (C1BAL10, C2BAL2, C4BAL1, and C4BAL5) were able to produce BLIS anti-Listeria, extracellular proteinases, and of these C2BAL2 and C4BAL1 were characterized as rapid acidifying cultures. After molecular testes were identified as Lactobacillus acidophilus and Lactococcus lactis. The results suggest two strains as potential candidates in the biopreservation of dairy products. |
| first_indexed | 2024-04-09T15:57:44Z |
| format | Article |
| id | doaj.art-d5299dc8e6d647b1a7d047c7455e29a0 |
| institution | Directory Open Access Journal |
| issn | 0100-3674 2238-6416 |
| language | English |
| last_indexed | 2024-04-09T15:57:44Z |
| publishDate | 2023-04-01 |
| publisher | Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
| record_format | Article |
| series | Revista do Instituto de Latícinios Cândido Tostes |
| spelling | doaj.art-d5299dc8e6d647b1a7d047c7455e29a02023-04-25T14:48:11ZengEmpresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)Revista do Instituto de Latícinios Cândido Tostes0100-36742238-64162023-04-01771213110.14295/2238-6416.v77i1.885493Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of BrazilCarlos Henrique Gomes de Sousa LimaNádia CarboneraElizabete HelbigThe present study aimed to evaluate the safety, antimicrobial and technological properties of lactic acid bacteria from artisanal Colonial-type cheese marketed at streets fair in Pelotas, RS. A total of 73 isolates were characterized as BAL after Gram’s method and catalase test. All strains were gelatinase and DNase negative, regarding hemolytic activity, seven were detected as α and two as β-hemolytic, exerting 63 BAL that demonstrated sensitivity to the chloramphenicol and tetracycline antibiotics. The antagonistic activity was verified by the spot-on-the-lawn method and the evaluation of the production of bacteriocins by the diffusion technique in wells. Four BAL (C1BAL10, C2BAL2, C4BAL1, and C4BAL5) were able to produce BLIS anti-Listeria, extracellular proteinases, and of these C2BAL2 and C4BAL1 were characterized as rapid acidifying cultures. After molecular testes were identified as Lactobacillus acidophilus and Lactococcus lactis. The results suggest two strains as potential candidates in the biopreservation of dairy products.https://www.revistadoilct.com.br/rilct/article/view/885antagonismopatogenicidadebacteriocinogênico. |
| spellingShingle | Carlos Henrique Gomes de Sousa Lima Nádia Carbonera Elizabete Helbig Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil Revista do Instituto de Latícinios Cândido Tostes antagonismo patogenicidade bacteriocinogênico. |
| title | Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil |
| title_full | Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil |
| title_fullStr | Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil |
| title_full_unstemmed | Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil |
| title_short | Antimicrobial and technological properties of lactic acid bacteria isolated from traditional Colonial-type cheese from the south of Brazil |
| title_sort | antimicrobial and technological properties of lactic acid bacteria isolated from traditional colonial type cheese from the south of brazil |
| topic | antagonismo patogenicidade bacteriocinogênico. |
| url | https://www.revistadoilct.com.br/rilct/article/view/885 |
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