Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers

Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples...

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Bibliographic Details
Main Authors: Qing Yang, Nicholas R Meyerson, Stephen K Clark, Camille L Paige, Will T Fattor, Alison R Gilchrist, Arturo Barbachano-Guerrero, Benjamin G Healy, Emma R Worden-Sapper, Sharon S Wu, Denise Muhlrad, Carolyn J Decker, Tassa K Saldi, Erika Lasda, Patrick Gonzales, Morgan R Fink, Kimngan L Tat, Cole R Hager, Jack C Davis, Christopher D Ozeroff, Gloria R Brisson, Matthew B McQueen, Leslie A Leinwand, Roy Parker, Sara L Sawyer
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-03-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/65113